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Phylogenetic and phenotypic analyses of streptomycetes isolated from local soil.

This study was to characterize the genetic and phenotypic features of soil isolates of Streptomyces. 39 isolates were harvested from various sites mainly around the University Agricultural Park, Universiti Putra Malaysia. Streptomyces griseus ATCC 10137 strain was also included for analysis as a ref...

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Main Authors: Tasrip, Nor Asmara, Mohd Desa, Mohd Nasir, Cheah, Yoke Kqueen, Chong, Pei Pei, Shamsudin, Mariana Nor
Format: Conference or Workshop Item
Language:English
English
Published: 2012
Online Access:http://psasir.upm.edu.my/id/eprint/27705/1/ID%2027705.pdf
http://psasir.upm.edu.my/id/eprint/27705/
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spelling my.upm.eprints.277052014-06-18T04:43:43Z http://psasir.upm.edu.my/id/eprint/27705/ Phylogenetic and phenotypic analyses of streptomycetes isolated from local soil. Tasrip, Nor Asmara Mohd Desa, Mohd Nasir Cheah, Yoke Kqueen Chong, Pei Pei Shamsudin, Mariana Nor This study was to characterize the genetic and phenotypic features of soil isolates of Streptomyces. 39 isolates were harvested from various sites mainly around the University Agricultural Park, Universiti Putra Malaysia. Streptomyces griseus ATCC 10137 strain was also included for analysis as a reference strain (n = 40). Basic morphological characteristics and 16S rDNA-sequence homology confirmed the isolates as Streptomyces. The later was used to construct a phylogenetic tree which showed the diverse evolutionary pedigree of the isolates in 6 major clusters. Based on ISP-2 media, the observed aerial mass-color of the isolates were categorized as yellow (n = 15), grey (n = 9), brown (n = 7), white (n = 6) and others (n = 3). Antibacterial activities were assessed on MHA and TSA by perpendicular streak method against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Salmonella sp. and Enterococcus sp. MHA demonstrated 33 isolates with antibacterial activity against at least one of the test bacteria while that on TSA accounted for only 24. Chi-square analysis in 2x2 contingency tables identified a potential correlation between aerial mass of non-white color and antibacterial activities. This study supports Streptomyces as the diverse organism with potential rich source of natural antimicrobial compounds. Nevertheless, the observed association between the aerial mass-color and antibacterial property, as well as the effect of different test media on the antibacterial activities needs to be further investigated. 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/27705/1/ID%2027705.pdf Tasrip, Nor Asmara and Mohd Desa, Mohd Nasir and Cheah, Yoke Kqueen and Chong, Pei Pei and Shamsudin, Mariana Nor (2012) Phylogenetic and phenotypic analyses of streptomycetes isolated from local soil. In: 3rd International Biotechnology and Biodiversity Conference & Exhibition (BIOJOHOR 2012), 9-11 June 2012, Johor Bahru. . English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description This study was to characterize the genetic and phenotypic features of soil isolates of Streptomyces. 39 isolates were harvested from various sites mainly around the University Agricultural Park, Universiti Putra Malaysia. Streptomyces griseus ATCC 10137 strain was also included for analysis as a reference strain (n = 40). Basic morphological characteristics and 16S rDNA-sequence homology confirmed the isolates as Streptomyces. The later was used to construct a phylogenetic tree which showed the diverse evolutionary pedigree of the isolates in 6 major clusters. Based on ISP-2 media, the observed aerial mass-color of the isolates were categorized as yellow (n = 15), grey (n = 9), brown (n = 7), white (n = 6) and others (n = 3). Antibacterial activities were assessed on MHA and TSA by perpendicular streak method against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Salmonella sp. and Enterococcus sp. MHA demonstrated 33 isolates with antibacterial activity against at least one of the test bacteria while that on TSA accounted for only 24. Chi-square analysis in 2x2 contingency tables identified a potential correlation between aerial mass of non-white color and antibacterial activities. This study supports Streptomyces as the diverse organism with potential rich source of natural antimicrobial compounds. Nevertheless, the observed association between the aerial mass-color and antibacterial property, as well as the effect of different test media on the antibacterial activities needs to be further investigated.
format Conference or Workshop Item
author Tasrip, Nor Asmara
Mohd Desa, Mohd Nasir
Cheah, Yoke Kqueen
Chong, Pei Pei
Shamsudin, Mariana Nor
spellingShingle Tasrip, Nor Asmara
Mohd Desa, Mohd Nasir
Cheah, Yoke Kqueen
Chong, Pei Pei
Shamsudin, Mariana Nor
Phylogenetic and phenotypic analyses of streptomycetes isolated from local soil.
author_facet Tasrip, Nor Asmara
Mohd Desa, Mohd Nasir
Cheah, Yoke Kqueen
Chong, Pei Pei
Shamsudin, Mariana Nor
author_sort Tasrip, Nor Asmara
title Phylogenetic and phenotypic analyses of streptomycetes isolated from local soil.
title_short Phylogenetic and phenotypic analyses of streptomycetes isolated from local soil.
title_full Phylogenetic and phenotypic analyses of streptomycetes isolated from local soil.
title_fullStr Phylogenetic and phenotypic analyses of streptomycetes isolated from local soil.
title_full_unstemmed Phylogenetic and phenotypic analyses of streptomycetes isolated from local soil.
title_sort phylogenetic and phenotypic analyses of streptomycetes isolated from local soil.
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/27705/1/ID%2027705.pdf
http://psasir.upm.edu.my/id/eprint/27705/
_version_ 1643829256174698496
score 13.211869

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