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Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis

40 Streptomyces isolates were recovered from soils taken from various sites around Universiti Putra Malaysia (UPM) and nearby peripheral areas, including 4 from a far-off location. Identification was done by conventional and molecular approaches (16S rRNA and rpoB genes sequence homology). All isola...

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Main Authors: Tasrip, Nor Asmara, Mohd Desa, Mohd Nasir, Cheah, Yoke Kqueen, Chong, Pei Pei, Shamsudin, Mariana Nor
Format: Conference or Workshop Item
Language:English
Published: 2012
Online Access:http://psasir.upm.edu.my/id/eprint/27407/1/ID%2027407.pdf
http://psasir.upm.edu.my/id/eprint/27407/
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spelling my.upm.eprints.274072018-04-24T04:04:22Z http://psasir.upm.edu.my/id/eprint/27407/ Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis Tasrip, Nor Asmara Mohd Desa, Mohd Nasir Cheah, Yoke Kqueen Chong, Pei Pei Shamsudin, Mariana Nor 40 Streptomyces isolates were recovered from soils taken from various sites around Universiti Putra Malaysia (UPM) and nearby peripheral areas, including 4 from a far-off location. Identification was done by conventional and molecular approaches (16S rRNA and rpoB genes sequence homology). All isolates were subjected to antibacterial assay against 2 pathogenic bacteria of gram positive and gram negative (Escherichia coli ATCC 25922 & Staphylococcus aureus ATCC 25923), by perpendicular streak method using MHA and TSA as the test media. With MHA as the test media in the antibacterial assay, > 50 % of the isolates showed antibacterial activity to at least one of the test bacteria as compared by using TSA as the test media. Color series of aerial mycelium was described based on growth on ISP-2 medium; there was a significant association between grey and non-grey color series of the aerial mycelium in association with the antibacterial activity of the isolates on MHA (89% vs. 42% respectively, P = 0.013) and TSA (78% vs. 35% respectively, P = 0.025). As a conclusion, different chemical composition of the test media could have an effect on the outcome of the antibacterial assay (MHA vs. TSA). The observed relationship between aerial mass-color (grey vs. nongrey) and antibacterial activity warrant further investigation in a larger sample of isolates. 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/27407/1/ID%2027407.pdf Tasrip, Nor Asmara and Mohd Desa, Mohd Nasir and Cheah, Yoke Kqueen and Chong, Pei Pei and Shamsudin, Mariana Nor (2012) Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis. In: 4th International Conference on the Development of Biomedical Engineering, 8-12 Jan. 2012, Ho Chi Minh, Vietnam. (pp. 135-136). 10.1007/978-3-642-32183-2_35
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description 40 Streptomyces isolates were recovered from soils taken from various sites around Universiti Putra Malaysia (UPM) and nearby peripheral areas, including 4 from a far-off location. Identification was done by conventional and molecular approaches (16S rRNA and rpoB genes sequence homology). All isolates were subjected to antibacterial assay against 2 pathogenic bacteria of gram positive and gram negative (Escherichia coli ATCC 25922 & Staphylococcus aureus ATCC 25923), by perpendicular streak method using MHA and TSA as the test media. With MHA as the test media in the antibacterial assay, > 50 % of the isolates showed antibacterial activity to at least one of the test bacteria as compared by using TSA as the test media. Color series of aerial mycelium was described based on growth on ISP-2 medium; there was a significant association between grey and non-grey color series of the aerial mycelium in association with the antibacterial activity of the isolates on MHA (89% vs. 42% respectively, P = 0.013) and TSA (78% vs. 35% respectively, P = 0.025). As a conclusion, different chemical composition of the test media could have an effect on the outcome of the antibacterial assay (MHA vs. TSA). The observed relationship between aerial mass-color (grey vs. nongrey) and antibacterial activity warrant further investigation in a larger sample of isolates.
format Conference or Workshop Item
author Tasrip, Nor Asmara
Mohd Desa, Mohd Nasir
Cheah, Yoke Kqueen
Chong, Pei Pei
Shamsudin, Mariana Nor
spellingShingle Tasrip, Nor Asmara
Mohd Desa, Mohd Nasir
Cheah, Yoke Kqueen
Chong, Pei Pei
Shamsudin, Mariana Nor
Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis
author_facet Tasrip, Nor Asmara
Mohd Desa, Mohd Nasir
Cheah, Yoke Kqueen
Chong, Pei Pei
Shamsudin, Mariana Nor
author_sort Tasrip, Nor Asmara
title Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis
title_short Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis
title_full Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis
title_fullStr Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis
title_full_unstemmed Associations between cultural characteristics and antibacterial activities of Streptomyces isolates: a preliminary analysis
title_sort associations between cultural characteristics and antibacterial activities of streptomyces isolates: a preliminary analysis
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/27407/1/ID%2027407.pdf
http://psasir.upm.edu.my/id/eprint/27407/
_version_ 1643829174011428864
score 13.211869

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