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Identification of vibrio species isolated from marine fish using polymerase chain reaction

Vibrio species are found in marine and estuarine environments. Vibriosis can cause more than 50% mortality in fish culture facilities once an outbreak is in progress. The objectives of this study were to subculture and identify Vibrio spp. that were isolated previously from marine fish; to develop a...

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Main Authors: Bahari, Muhamad Faiz, Mohd Yusoff, Sabri
Format: Conference or Workshop Item
Language:English
Published: 2011
Online Access:http://psasir.upm.edu.my/id/eprint/27306/1/Proceedings%2038.pdf
http://psasir.upm.edu.my/id/eprint/27306/
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spelling my.upm.eprints.273062015-01-07T02:38:57Z http://psasir.upm.edu.my/id/eprint/27306/ Identification of vibrio species isolated from marine fish using polymerase chain reaction Bahari, Muhamad Faiz Mohd Yusoff, Sabri Vibrio species are found in marine and estuarine environments. Vibriosis can cause more than 50% mortality in fish culture facilities once an outbreak is in progress. The objectives of this study were to subculture and identify Vibrio spp. that were isolated previously from marine fish; to develop a technique for simultaneous identification of several Vibrio spp. (V. alginolyticus, V. parahaemolyticus, V. fluvialis and V. vulnificus) using polymerase chain reaction (PCR); and to compare the rapid identification kit and PCRtechniques commonly used for the identification of the Vibrio spp. In this study, 20 isolates from four Vibrio species, which consisted of five eachof V. alginolyticus, V. parahaemolyticus, V. fluvialis and V. vulnificus isolates were provided by National Fish Health Research Centre (NaFisH). The species of Vibrio were identified using an identification kit, API 20E system. These organisms were isolated from various marine fish such as Asian Seabass (Latescalcarifer), Grouper (Epinepheluscoioides), Silver Pomfret (Pampusargenteus) and Red Snapper (Lutjanuscampechanus). The isolates were previously stored at -80°C and subcultured onto TSA+. The pure cultures were then transferred to TSB+. These isolates were subjected to DNA extraction. Once the DNA is ready, PCR was used to optimise the products with the designated primers. All the PCR products were electrophoresed through 1% agarose gel for 1 h. The designated primers in this study were found suitable for the detection of V. alginolyticus,V. parahaemolyticus,V. fluvialis and V. vulnificus.Using the API 20E system, 15% (3/20) isolatesof Vibrio spp. were negative, indicating the the PCR technique is still required to confirm the result obtained by the use of the API 20E system. 2011-01-11 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/27306/1/Proceedings%2038.pdf Bahari, Muhamad Faiz and Mohd Yusoff, Sabri (2011) Identification of vibrio species isolated from marine fish using polymerase chain reaction. In: 6th Seminar on Veterinary Sciences, 11-14 Jan. 2011, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (p. 129).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Vibrio species are found in marine and estuarine environments. Vibriosis can cause more than 50% mortality in fish culture facilities once an outbreak is in progress. The objectives of this study were to subculture and identify Vibrio spp. that were isolated previously from marine fish; to develop a technique for simultaneous identification of several Vibrio spp. (V. alginolyticus, V. parahaemolyticus, V. fluvialis and V. vulnificus) using polymerase chain reaction (PCR); and to compare the rapid identification kit and PCRtechniques commonly used for the identification of the Vibrio spp. In this study, 20 isolates from four Vibrio species, which consisted of five eachof V. alginolyticus, V. parahaemolyticus, V. fluvialis and V. vulnificus isolates were provided by National Fish Health Research Centre (NaFisH). The species of Vibrio were identified using an identification kit, API 20E system. These organisms were isolated from various marine fish such as Asian Seabass (Latescalcarifer), Grouper (Epinepheluscoioides), Silver Pomfret (Pampusargenteus) and Red Snapper (Lutjanuscampechanus). The isolates were previously stored at -80°C and subcultured onto TSA+. The pure cultures were then transferred to TSB+. These isolates were subjected to DNA extraction. Once the DNA is ready, PCR was used to optimise the products with the designated primers. All the PCR products were electrophoresed through 1% agarose gel for 1 h. The designated primers in this study were found suitable for the detection of V. alginolyticus,V. parahaemolyticus,V. fluvialis and V. vulnificus.Using the API 20E system, 15% (3/20) isolatesof Vibrio spp. were negative, indicating the the PCR technique is still required to confirm the result obtained by the use of the API 20E system.
format Conference or Workshop Item
author Bahari, Muhamad Faiz
Mohd Yusoff, Sabri
spellingShingle Bahari, Muhamad Faiz
Mohd Yusoff, Sabri
Identification of vibrio species isolated from marine fish using polymerase chain reaction
author_facet Bahari, Muhamad Faiz
Mohd Yusoff, Sabri
author_sort Bahari, Muhamad Faiz
title Identification of vibrio species isolated from marine fish using polymerase chain reaction
title_short Identification of vibrio species isolated from marine fish using polymerase chain reaction
title_full Identification of vibrio species isolated from marine fish using polymerase chain reaction
title_fullStr Identification of vibrio species isolated from marine fish using polymerase chain reaction
title_full_unstemmed Identification of vibrio species isolated from marine fish using polymerase chain reaction
title_sort identification of vibrio species isolated from marine fish using polymerase chain reaction
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/27306/1/Proceedings%2038.pdf
http://psasir.upm.edu.my/id/eprint/27306/
_version_ 1643829145677856768
score 13.211869

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