Method for isolating DNA
The present invention provides a method for the isolation of nucleic acid from microbial cells in an environmental sample. The method includes preparing a suspension of the environmental sample, lysing the suspended sample with a buffered solution, adding sodium dodecylsufate solution to the lysed s...
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| Format: | Patent |
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2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/26962/ |
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my.upm.eprints.269622016-09-08T02:37:25Z http://psasir.upm.edu.my/id/eprint/26962/ Method for isolating DNA Hassan, Mohd Ali Tabatabaei, Meisam Zakaria @ Mamat, Mohd Rafein Abdul Rahim, Raha Wright, Andre-Denis G. Shirai, Yoshihito Abdullah, Norhani Shamsara, Mehdi Sakai, Kenji The present invention provides a method for the isolation of nucleic acid from microbial cells in an environmental sample. The method includes preparing a suspension of the environmental sample, lysing the suspended sample with a buffered solution, adding sodium dodecylsufate solution to the lysed suspended sample, carrying out solvent extraction and separation to obtain an aqueous phase, reacting the aqueous phase with solvents to generate an insoluble precipitate containing nucleic acid, and isolating the nucleic acid therefrom, thereby releasing high molecular weight nucleic acid pellets from the cells. 2008-07-30 Patent NonPeerReviewed Mohd Ali Hassan (2008) Method for isolating DNA. UNSPECIFIED. |
| institution |
Universiti Putra Malaysia |
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| continent |
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| country |
Malaysia |
| content_provider |
Universiti Putra Malaysia |
| content_source |
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http://psasir.upm.edu.my/ |
| description |
The present invention provides a method for the isolation of nucleic acid from microbial cells in an environmental sample. The method includes preparing a suspension of the environmental sample, lysing the suspended sample with a buffered solution, adding sodium dodecylsufate solution to the lysed suspended sample, carrying out solvent extraction and separation to obtain an aqueous phase, reacting the aqueous phase with solvents to generate an insoluble precipitate containing nucleic acid, and isolating the nucleic acid therefrom, thereby releasing high molecular weight nucleic acid pellets from the cells. |
| format |
Patent |
| author |
Hassan, Mohd Ali Tabatabaei, Meisam Zakaria @ Mamat, Mohd Rafein Abdul Rahim, Raha Wright, Andre-Denis G. Shirai, Yoshihito Abdullah, Norhani Shamsara, Mehdi Sakai, Kenji |
| spellingShingle |
Hassan, Mohd Ali Tabatabaei, Meisam Zakaria @ Mamat, Mohd Rafein Abdul Rahim, Raha Wright, Andre-Denis G. Shirai, Yoshihito Abdullah, Norhani Shamsara, Mehdi Sakai, Kenji Method for isolating DNA |
| author_facet |
Hassan, Mohd Ali Tabatabaei, Meisam Zakaria @ Mamat, Mohd Rafein Abdul Rahim, Raha Wright, Andre-Denis G. Shirai, Yoshihito Abdullah, Norhani Shamsara, Mehdi Sakai, Kenji |
| author_sort |
Hassan, Mohd Ali |
| title |
Method for isolating DNA |
| title_short |
Method for isolating DNA |
| title_full |
Method for isolating DNA |
| title_fullStr |
Method for isolating DNA |
| title_full_unstemmed |
Method for isolating DNA |
| title_sort |
method for isolating dna |
| publishDate |
2008 |
| url |
http://psasir.upm.edu.my/id/eprint/26962/ |
| _version_ |
1643829056774340608 |
| score |
13.211869 |