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Fecal progestin extraction and analysis for non-invasive monitoring of ovarian cycle in beef cows

The aims of the present study were to determine presence of immunoreactive progestins in feces, correlate fecal progestins with plasma progesterone (P 4) concentrations and subsequently assess the role of fecal progestins in monitoring estrous cycle in Kedah Kelantan (KK) beef cows. A total of 12 cy...

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Main Authors: Yimer, Nurhusien, Yusoff, Rosnina, Haron, Abd Wahid, Bukar, Muhammad Modu, Malik, Abdul, Yap, Keng Chee, Fahmi, M., Ganesamurthi, Perumal, Saharee, Abdul Aziz
Format: Article
Language:English
Published: Faculty of Veterinary Science, University of Agriculture, Faisalabad 2012
Online Access:http://psasir.upm.edu.my/id/eprint/25945/1/Fecal%20progestin%20extraction%20and%20analysis%20for%20non.pdf
http://psasir.upm.edu.my/id/eprint/25945/
http://www.pvj.com.pk/archive/Volume_32_Issue_4_2012.htm
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spelling my.upm.eprints.259452016-12-01T09:08:34Z http://psasir.upm.edu.my/id/eprint/25945/ Fecal progestin extraction and analysis for non-invasive monitoring of ovarian cycle in beef cows Yimer, Nurhusien Yusoff, Rosnina Haron, Abd Wahid Bukar, Muhammad Modu Malik, Abdul Yap, Keng Chee Fahmi, M. Ganesamurthi, Perumal Saharee, Abdul Aziz The aims of the present study were to determine presence of immunoreactive progestins in feces, correlate fecal progestins with plasma progesterone (P 4) concentrations and subsequently assess the role of fecal progestins in monitoring estrous cycle in Kedah Kelantan (KK) beef cows. A total of 12 cycling cows were subjected to blood and matched fecal sampling twice a week for 9 weeks. The concentrations of plasma P 4 and fecal progestins extracted using a modified technique, were determined by a P 4 radioimmunoassay (RIA) kit. There was a significant positive correlation between the concentrations of fecal progestins and plasma P 4 (r = 0.6, P<0.01), as tested for the whole group except one animal. High performance liquid chromatographic separation of fecal extracts and subsequent radioimmunoassay revealed presence of four immunoreactive progestins against the P 4 antibodies. These results imply that the non-invasive measure of fecal progestins using a DSL-3900 RIA kit can be used to monitor the ovarian activity in beef cows. Faculty of Veterinary Science, University of Agriculture, Faisalabad 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25945/1/Fecal%20progestin%20extraction%20and%20analysis%20for%20non.pdf Yimer, Nurhusien and Yusoff, Rosnina and Haron, Abd Wahid and Bukar, Muhammad Modu and Malik, Abdul and Yap, Keng Chee and Fahmi, M. and Ganesamurthi, Perumal and Saharee, Abdul Aziz (2012) Fecal progestin extraction and analysis for non-invasive monitoring of ovarian cycle in beef cows. Pakistan Veterinary Journal, 32 (4). pp. 584-588. ISSN 0253-8318; ESSN: 2074-7764 http://www.pvj.com.pk/archive/Volume_32_Issue_4_2012.htm
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The aims of the present study were to determine presence of immunoreactive progestins in feces, correlate fecal progestins with plasma progesterone (P 4) concentrations and subsequently assess the role of fecal progestins in monitoring estrous cycle in Kedah Kelantan (KK) beef cows. A total of 12 cycling cows were subjected to blood and matched fecal sampling twice a week for 9 weeks. The concentrations of plasma P 4 and fecal progestins extracted using a modified technique, were determined by a P 4 radioimmunoassay (RIA) kit. There was a significant positive correlation between the concentrations of fecal progestins and plasma P 4 (r = 0.6, P<0.01), as tested for the whole group except one animal. High performance liquid chromatographic separation of fecal extracts and subsequent radioimmunoassay revealed presence of four immunoreactive progestins against the P 4 antibodies. These results imply that the non-invasive measure of fecal progestins using a DSL-3900 RIA kit can be used to monitor the ovarian activity in beef cows.
format Article
author Yimer, Nurhusien
Yusoff, Rosnina
Haron, Abd Wahid
Bukar, Muhammad Modu
Malik, Abdul
Yap, Keng Chee
Fahmi, M.
Ganesamurthi, Perumal
Saharee, Abdul Aziz
spellingShingle Yimer, Nurhusien
Yusoff, Rosnina
Haron, Abd Wahid
Bukar, Muhammad Modu
Malik, Abdul
Yap, Keng Chee
Fahmi, M.
Ganesamurthi, Perumal
Saharee, Abdul Aziz
Fecal progestin extraction and analysis for non-invasive monitoring of ovarian cycle in beef cows
author_facet Yimer, Nurhusien
Yusoff, Rosnina
Haron, Abd Wahid
Bukar, Muhammad Modu
Malik, Abdul
Yap, Keng Chee
Fahmi, M.
Ganesamurthi, Perumal
Saharee, Abdul Aziz
author_sort Yimer, Nurhusien
title Fecal progestin extraction and analysis for non-invasive monitoring of ovarian cycle in beef cows
title_short Fecal progestin extraction and analysis for non-invasive monitoring of ovarian cycle in beef cows
title_full Fecal progestin extraction and analysis for non-invasive monitoring of ovarian cycle in beef cows
title_fullStr Fecal progestin extraction and analysis for non-invasive monitoring of ovarian cycle in beef cows
title_full_unstemmed Fecal progestin extraction and analysis for non-invasive monitoring of ovarian cycle in beef cows
title_sort fecal progestin extraction and analysis for non-invasive monitoring of ovarian cycle in beef cows
publisher Faculty of Veterinary Science, University of Agriculture, Faisalabad
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/25945/1/Fecal%20progestin%20extraction%20and%20analysis%20for%20non.pdf
http://psasir.upm.edu.my/id/eprint/25945/
http://www.pvj.com.pk/archive/Volume_32_Issue_4_2012.htm
_version_ 1643828789461909504
score 13.211869

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