Leptospira kmetyi sp. nov., isolated from an environmental source in Malaysia.
A single Leptospira strain (designated Bejo-Iso9T) was isolated from a soil sample taken in Johor, Malaysia. The isolate showed motility and morphology typical of the genus Leptospira under darkfield microscopy. Cells were found to be 10–13 mm in length and 0.2 mm in diameter, with a wavelength o...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Society for General Microbiology
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25683/ |
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| Summary: | A single Leptospira strain (designated Bejo-Iso9T) was isolated from a soil sample taken in Johor,
Malaysia. The isolate showed motility and morphology typical of the genus Leptospira under darkfield
microscopy. Cells were found to be 10–13 mm in length and 0.2 mm in diameter, with a
wavelength of 0.5 mm and an amplitude of approximately 0.2 mm. Phenotypically, strain Bejo-
Iso9T grew in Ellinghausen–McCullough–Johnson–Harris medium at 13, 30 and 37 6C, and also
in the presence of 8-azaguanine. Serologically, strain Bejo-Iso9T produced titres towards several
members of the Tarassovi serogroup, but was found to be serologically unique by cross-agglutinin
absorption test and thus represented a novel serovar. The proposed name for this serovar is
Malaysia. Phylogenetic analysis of 16S rRNA gene sequences placed this novel strain within the
radiation of the genus Leptospira, with sequence similarities within the range 90.4–99.5% with
respect to recognized Leptospira species. DNA–DNA hybridization against the three most closely
related Leptospira species was used to confirm the results of the 16S rRNA gene sequence
analysis. The G+C content of the genome of strain Bejo-Iso9T was 36.2 mol%. On the basis of
phenotypic, serological and phylogenetic data, strain Bejo-Iso9T represents a novel species of the
genus Leptospira, for which the name Leptospira kmetyi sp. nov. is proposed. The type strain is
Bejo-Iso9T (5WHO LT1101T5KIT Bejo-Iso9T). |
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