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Molecular characterization of Pasteurella multocida isolates from rabbits

Forty isolates of Pasteurella multocida from healthy (17 isolates) and diseased (23 isolates) rabbits were assayed for the presence of plasmids in seeking to determine whether any correlation exists between the presence of plasmids and health status, sensitivity to antimicrobial agents, capsular and...

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Main Authors: Al-Haddawi, Muthafar Hussain, Jasni, Sabri, Radu, Son, Mutalib, Abdul Rahim, Bahaman, Abdul Rani, Saad, Mohd Zamri, Sheikh Omar, Abdul Rahman
Format: Article
Language:English
English
Published: Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 1999
Online Access:http://psasir.upm.edu.my/id/eprint/25446/1/Molecular%20characterization%20of%20Pasteurella%20multocida%20isolates%20from%20rabbits.pdf
http://psasir.upm.edu.my/id/eprint/25446/7/45_269.pdf
http://psasir.upm.edu.my/id/eprint/25446/
https://www.jstage.jst.go.jp/article/jgam/45/6/45_6_269/_article
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spelling my.upm.eprints.254462024-08-07T07:29:48Z http://psasir.upm.edu.my/id/eprint/25446/ Molecular characterization of Pasteurella multocida isolates from rabbits Al-Haddawi, Muthafar Hussain Jasni, Sabri Radu, Son Mutalib, Abdul Rahim Bahaman, Abdul Rani Saad, Mohd Zamri Sheikh Omar, Abdul Rahman Forty isolates of Pasteurella multocida from healthy (17 isolates) and diseased (23 isolates) rabbits were assayed for the presence of plasmids in seeking to determine whether any correlation exists between the presence of plasmids and health status, sensitivity to antimicrobial agents, capsular and somatic type, and the anatomic site of isolation. Six isolates were found harboring plasmids. A similar ladder pattern ranging from 18 to 3 megadalton (Mda) were found in three isolates recovered from diseased rabbits. One band of molecular weight 6.6 Mda was shared by four of five (4/5) isolates from the diseased rabbits. No correlation was found between the presence of the common plasmids and serotype, resistance to antimicrobial agents, and anatomic sites from which the bacteria were cultured. Random amplification polymorphic DNA was applied to subtype all the isolates of P. multocida. Two single primers were tested for their abilities to generate individual fingerprints by using PCR. Primer 1 grouped the isolates into 7 profiles, and primer 2 grouped them into 15. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) results show the presence of a wide heterogeneity within P. multocida isolates. Therefore RAPD-PCR is an efficient technique to detect the DNA polymorphism and could be used to discriminate P. multocida of rabbit isolates together with serologic typing. Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/25446/1/Molecular%20characterization%20of%20Pasteurella%20multocida%20isolates%20from%20rabbits.pdf text en http://psasir.upm.edu.my/id/eprint/25446/7/45_269.pdf Al-Haddawi, Muthafar Hussain and Jasni, Sabri and Radu, Son and Mutalib, Abdul Rahim and Bahaman, Abdul Rani and Saad, Mohd Zamri and Sheikh Omar, Abdul Rahman (1999) Molecular characterization of Pasteurella multocida isolates from rabbits. Journal of General and Applied Microbiology, 45 (6). pp. 269-275. ISSN 0022-1260; ESSN: 1349-8037 https://www.jstage.jst.go.jp/article/jgam/45/6/45_6_269/_article 10.2323/jgam.45.269
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Forty isolates of Pasteurella multocida from healthy (17 isolates) and diseased (23 isolates) rabbits were assayed for the presence of plasmids in seeking to determine whether any correlation exists between the presence of plasmids and health status, sensitivity to antimicrobial agents, capsular and somatic type, and the anatomic site of isolation. Six isolates were found harboring plasmids. A similar ladder pattern ranging from 18 to 3 megadalton (Mda) were found in three isolates recovered from diseased rabbits. One band of molecular weight 6.6 Mda was shared by four of five (4/5) isolates from the diseased rabbits. No correlation was found between the presence of the common plasmids and serotype, resistance to antimicrobial agents, and anatomic sites from which the bacteria were cultured. Random amplification polymorphic DNA was applied to subtype all the isolates of P. multocida. Two single primers were tested for their abilities to generate individual fingerprints by using PCR. Primer 1 grouped the isolates into 7 profiles, and primer 2 grouped them into 15. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) results show the presence of a wide heterogeneity within P. multocida isolates. Therefore RAPD-PCR is an efficient technique to detect the DNA polymorphism and could be used to discriminate P. multocida of rabbit isolates together with serologic typing.
format Article
author Al-Haddawi, Muthafar Hussain
Jasni, Sabri
Radu, Son
Mutalib, Abdul Rahim
Bahaman, Abdul Rani
Saad, Mohd Zamri
Sheikh Omar, Abdul Rahman
spellingShingle Al-Haddawi, Muthafar Hussain
Jasni, Sabri
Radu, Son
Mutalib, Abdul Rahim
Bahaman, Abdul Rani
Saad, Mohd Zamri
Sheikh Omar, Abdul Rahman
Molecular characterization of Pasteurella multocida isolates from rabbits
author_facet Al-Haddawi, Muthafar Hussain
Jasni, Sabri
Radu, Son
Mutalib, Abdul Rahim
Bahaman, Abdul Rani
Saad, Mohd Zamri
Sheikh Omar, Abdul Rahman
author_sort Al-Haddawi, Muthafar Hussain
title Molecular characterization of Pasteurella multocida isolates from rabbits
title_short Molecular characterization of Pasteurella multocida isolates from rabbits
title_full Molecular characterization of Pasteurella multocida isolates from rabbits
title_fullStr Molecular characterization of Pasteurella multocida isolates from rabbits
title_full_unstemmed Molecular characterization of Pasteurella multocida isolates from rabbits
title_sort molecular characterization of pasteurella multocida isolates from rabbits
publisher Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
publishDate 1999
url http://psasir.upm.edu.my/id/eprint/25446/1/Molecular%20characterization%20of%20Pasteurella%20multocida%20isolates%20from%20rabbits.pdf
http://psasir.upm.edu.my/id/eprint/25446/7/45_269.pdf
http://psasir.upm.edu.my/id/eprint/25446/
https://www.jstage.jst.go.jp/article/jgam/45/6/45_6_269/_article
_version_ 1807051104033177600
score 13.211869

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