Cover Image

Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly.

Elucidating the mechanism of genetic exchange is fundamental for understanding how genes for such traits as virulence, disease phenotype, and drug resistance are transferred between pathogen strains. Genetic exchange occurs in the parasitic protists Trypanosoma brucei, T. cruzi, and Leishmania major...

Full description

Saved in:
Bibliographic Details
Main Authors: Peacock, L., Ferris, V., Sharma, Reuben Sunil Kumar, Sunter, J., Bailey, M., Carrington, M., Gibson, W.
Format: Article
Language:English
English
Published: National Academy of Sciences 2011
Online Access:http://psasir.upm.edu.my/id/eprint/25359/1/Identification%20of%20the%20meiotic%20life%20cycle%20stage%20of%20Trypanosoma%20brucei%20in%20the%20tsetse%20fly.pdf
http://psasir.upm.edu.my/id/eprint/25359/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.25359
record_format eprints
spelling my.upm.eprints.253592015-10-28T07:12:17Z http://psasir.upm.edu.my/id/eprint/25359/ Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly. Peacock, L. Ferris, V. Sharma, Reuben Sunil Kumar Sunter, J. Bailey, M. Carrington, M. Gibson, W. Elucidating the mechanism of genetic exchange is fundamental for understanding how genes for such traits as virulence, disease phenotype, and drug resistance are transferred between pathogen strains. Genetic exchange occurs in the parasitic protists Trypanosoma brucei, T. cruzi, and Leishmania major, but the precise cellular mechanisms are unknown, because the process has not been observed directly. Here we exploit the identification of homologs of meiotic genes in the T. brucei genome and demonstrate that three functionally distinct, meiosis-specific proteins are expressed in the nucleus of a single specific cell type, defining a previously undescribed developmental stage occurring within the tsetse fly salivary gland. Expression occurs in clonal and mixed infections, indicating that the meiotic program is an intrinsic but hitherto cryptic part of the developmental cycle of trypanosomes. In experimental crosses, expression of meiosis-specific proteins usually occurred before cell fusion. This is evidence of conventional meiotic division in an excavate protist, and the functional conservation of the meiotic machinery in these divergent organisms underlines the ubiquity and basal evolution of meiosis in eukaryotes. National Academy of Sciences 2011-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25359/1/Identification%20of%20the%20meiotic%20life%20cycle%20stage%20of%20Trypanosoma%20brucei%20in%20the%20tsetse%20fly.pdf Peacock, L. and Ferris, V. and Sharma, Reuben Sunil Kumar and Sunter, J. and Bailey, M. and Carrington, M. and Gibson, W. (2011) Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 108 (9). pp. 3671-3676. ISSN 0027-8424 10.1073/pnas.1019423108 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Elucidating the mechanism of genetic exchange is fundamental for understanding how genes for such traits as virulence, disease phenotype, and drug resistance are transferred between pathogen strains. Genetic exchange occurs in the parasitic protists Trypanosoma brucei, T. cruzi, and Leishmania major, but the precise cellular mechanisms are unknown, because the process has not been observed directly. Here we exploit the identification of homologs of meiotic genes in the T. brucei genome and demonstrate that three functionally distinct, meiosis-specific proteins are expressed in the nucleus of a single specific cell type, defining a previously undescribed developmental stage occurring within the tsetse fly salivary gland. Expression occurs in clonal and mixed infections, indicating that the meiotic program is an intrinsic but hitherto cryptic part of the developmental cycle of trypanosomes. In experimental crosses, expression of meiosis-specific proteins usually occurred before cell fusion. This is evidence of conventional meiotic division in an excavate protist, and the functional conservation of the meiotic machinery in these divergent organisms underlines the ubiquity and basal evolution of meiosis in eukaryotes.
format Article
author Peacock, L.
Ferris, V.
Sharma, Reuben Sunil Kumar
Sunter, J.
Bailey, M.
Carrington, M.
Gibson, W.
spellingShingle Peacock, L.
Ferris, V.
Sharma, Reuben Sunil Kumar
Sunter, J.
Bailey, M.
Carrington, M.
Gibson, W.
Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly.
author_facet Peacock, L.
Ferris, V.
Sharma, Reuben Sunil Kumar
Sunter, J.
Bailey, M.
Carrington, M.
Gibson, W.
author_sort Peacock, L.
title Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly.
title_short Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly.
title_full Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly.
title_fullStr Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly.
title_full_unstemmed Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly.
title_sort identification of the meiotic life cycle stage of trypanosoma brucei in the tsetse fly.
publisher National Academy of Sciences
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/25359/1/Identification%20of%20the%20meiotic%20life%20cycle%20stage%20of%20Trypanosoma%20brucei%20in%20the%20tsetse%20fly.pdf
http://psasir.upm.edu.my/id/eprint/25359/
_version_ 1643828638353719296
score 13.211869

Similar Items