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In vitro intracellular trafficking of biodegradable nanoparticles dextran spermine in cancer cell lines.

The objective of the present study is to evaluate the effect of cationic dextran on the proliferation rate and biosynthetic activities of HT29, a human colonic adenocarcinoma, and MCF7, a human breast cancer cell line. Cationic dextran was prepared by means of reductive-amination between oxidised de...

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Main Authors: Abedini, Fatemeh, Hosseinkhani, Hossein, Ismail , Maznah, Chen, Yi Ru, Omar, Abdul Rahman, Chong, Pei Pei, Domb, Abraham J.
Format: Article
Language:English
English
Published: Inderscience Publisher 2011
Online Access:http://psasir.upm.edu.my/id/eprint/25341/1/In%20vitro%20intracellular%20trafficking%20of%20biodegradable%20nanoparticles%20dextran%20spermine%20in%20cancer%20cell%20lines.pdf
http://psasir.upm.edu.my/id/eprint/25341/
http://www.inderscience.com/
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spelling my.upm.eprints.253412015-10-09T07:08:03Z http://psasir.upm.edu.my/id/eprint/25341/ In vitro intracellular trafficking of biodegradable nanoparticles dextran spermine in cancer cell lines. Abedini, Fatemeh Hosseinkhani, Hossein Ismail , Maznah Chen, Yi Ru Omar, Abdul Rahman Chong, Pei Pei Domb, Abraham J. The objective of the present study is to evaluate the effect of cationic dextran on the proliferation rate and biosynthetic activities of HT29, a human colonic adenocarcinoma, and MCF7, a human breast cancer cell line. Cationic dextran was prepared by means of reductive-amination between oxidised dextran and the natural oligoamine, spermine. Biological evaluations including cell proliferation assay, and cell cycle were studied. Flow cytometery was performed in order to determine the biological behaviour of cationic dextran after internalised into the cells. Our results clearly indicated that the cationic dextran was not toxic to the cells when the concentration was 5 μg/ml or lower. The results of the cell cycle flow cytometery indicated that the means of R2 in HT29, MCF7 and HeLa cells were less than 5 three days after treatment with 5 μg/ml of cationic dextran. We conclude that the toxicity of cationic dextran is dose dependent and it is not toxic at concentration lower than 5 μg/ml, and tolerable by the cells, and it can be used as a tool for gene delivery. Inderscience Publisher 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25341/1/In%20vitro%20intracellular%20trafficking%20of%20biodegradable%20nanoparticles%20dextran%20spermine%20in%20cancer%20cell%20lines.pdf Abedini, Fatemeh and Hosseinkhani, Hossein and Ismail , Maznah and Chen, Yi Ru and Omar, Abdul Rahman and Chong, Pei Pei and Domb, Abraham J. (2011) In vitro intracellular trafficking of biodegradable nanoparticles dextran spermine in cancer cell lines. International Journal of Nanotechnology, 8 (8-9). pp. 712-723. ISSN 1475-7435 http://www.inderscience.com/ 10.1504/IJNT.2011.041440 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The objective of the present study is to evaluate the effect of cationic dextran on the proliferation rate and biosynthetic activities of HT29, a human colonic adenocarcinoma, and MCF7, a human breast cancer cell line. Cationic dextran was prepared by means of reductive-amination between oxidised dextran and the natural oligoamine, spermine. Biological evaluations including cell proliferation assay, and cell cycle were studied. Flow cytometery was performed in order to determine the biological behaviour of cationic dextran after internalised into the cells. Our results clearly indicated that the cationic dextran was not toxic to the cells when the concentration was 5 μg/ml or lower. The results of the cell cycle flow cytometery indicated that the means of R2 in HT29, MCF7 and HeLa cells were less than 5 three days after treatment with 5 μg/ml of cationic dextran. We conclude that the toxicity of cationic dextran is dose dependent and it is not toxic at concentration lower than 5 μg/ml, and tolerable by the cells, and it can be used as a tool for gene delivery.
format Article
author Abedini, Fatemeh
Hosseinkhani, Hossein
Ismail , Maznah
Chen, Yi Ru
Omar, Abdul Rahman
Chong, Pei Pei
Domb, Abraham J.
spellingShingle Abedini, Fatemeh
Hosseinkhani, Hossein
Ismail , Maznah
Chen, Yi Ru
Omar, Abdul Rahman
Chong, Pei Pei
Domb, Abraham J.
In vitro intracellular trafficking of biodegradable nanoparticles dextran spermine in cancer cell lines.
author_facet Abedini, Fatemeh
Hosseinkhani, Hossein
Ismail , Maznah
Chen, Yi Ru
Omar, Abdul Rahman
Chong, Pei Pei
Domb, Abraham J.
author_sort Abedini, Fatemeh
title In vitro intracellular trafficking of biodegradable nanoparticles dextran spermine in cancer cell lines.
title_short In vitro intracellular trafficking of biodegradable nanoparticles dextran spermine in cancer cell lines.
title_full In vitro intracellular trafficking of biodegradable nanoparticles dextran spermine in cancer cell lines.
title_fullStr In vitro intracellular trafficking of biodegradable nanoparticles dextran spermine in cancer cell lines.
title_full_unstemmed In vitro intracellular trafficking of biodegradable nanoparticles dextran spermine in cancer cell lines.
title_sort in vitro intracellular trafficking of biodegradable nanoparticles dextran spermine in cancer cell lines.
publisher Inderscience Publisher
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/25341/1/In%20vitro%20intracellular%20trafficking%20of%20biodegradable%20nanoparticles%20dextran%20spermine%20in%20cancer%20cell%20lines.pdf
http://psasir.upm.edu.my/id/eprint/25341/
http://www.inderscience.com/
_version_ 1643828633022758912
score 13.211869

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