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Neonatal feed restriction modulates circulating levels of corticosterone and expression of glucocorticoid receptor and heat shock protein 70 in aged Japanese quail exposed to acute heat stress.

This study aimed to determine the effect of neonatal feed restriction on plasma corticosterone concentration (CORT), hippocampal glucocorticoid receptor (GR) expression, and heat shock protein (Hsp) 70 expression in aged male Japanese quail subjected to acute heat stress. Equal numbers of chicks wer...

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Main Authors: A. F., Soleimani, Idrus, Zulkifli, Omar, Abdul Rahman, Abdul Rahim, Raha
Format: Article
Language:English
English
Published: Poultry Science Association Inc. 2011
Online Access:http://psasir.upm.edu.my/id/eprint/25337/1/Neonatal%20feed%20restriction%20modulates%20circulating%20levels%20of%20corticosterone%20and%20expression%20of%20glucocorticoid%20receptor%20and%20heat%20shock%20protein%2070%20in%20aged%20Japanese%20quail%20exposed%20to%20acute%20heat%20stress.pdf
http://psasir.upm.edu.my/id/eprint/25337/
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spelling my.upm.eprints.253372015-09-30T07:10:41Z http://psasir.upm.edu.my/id/eprint/25337/ Neonatal feed restriction modulates circulating levels of corticosterone and expression of glucocorticoid receptor and heat shock protein 70 in aged Japanese quail exposed to acute heat stress. A. F., Soleimani Idrus, Zulkifli Omar, Abdul Rahman Abdul Rahim, Raha This study aimed to determine the effect of neonatal feed restriction on plasma corticosterone concentration (CORT), hippocampal glucocorticoid receptor (GR) expression, and heat shock protein (Hsp) 70 expression in aged male Japanese quail subjected to acute heat stress. Equal numbers of chicks were subjected to either ad libitum feeding (AL) or 60% feed restriction on d 4, 5, and 6 (FR). At 21 (young) and 270 (aged) d of age, birds were exposed to 43 ± 1°C for 1 h. Blood and hippocampus samples were collected to determine CORT and Hsp 70 and GR expressions before heat stress and following 1 h of heat stress, 1 h of post-heat stress recovery, and 2 h of post-heat stress recovery. With the use of real-time PCR and enzyme immunoassay, we examined the hippocampal expression of GR and Hsp 70 and CORT. The GR expression of the young birds increased following heat stress and remained consistent throughout the period of recovery. Conversely, no significant changes were noted on GR expression of aged birds. Although both young and aged birds had similar CORT before and during heat stress, the latter exhibited greater values following 1 and 2 h of recovery. Within the young group, feeding regimens had no significant effect on Hsp 70 expression. However, neonatal feed restriction improved Hsp 70 expression in aged birds. Neonatal feed restriction, compared with the AL group, resulted in higher CORT on d 21 but the converse was noted on d 270. Neonatal feed restriction appears to set a robust reactive hypothalamo-pituitary-adrenal response allowing the development of adaptive, healthy, and resilient phenotypes in aged quail as measured by a higher hippocampal Hsp 70 expression along with lower CORT. Poultry Science Association Inc. 2011-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25337/1/Neonatal%20feed%20restriction%20modulates%20circulating%20levels%20of%20corticosterone%20and%20expression%20of%20glucocorticoid%20receptor%20and%20heat%20shock%20protein%2070%20in%20aged%20Japanese%20quail%20exposed%20to%20acute%20heat%20stress.pdf A. F., Soleimani and Idrus, Zulkifli and Omar, Abdul Rahman and Abdul Rahim, Raha (2011) Neonatal feed restriction modulates circulating levels of corticosterone and expression of glucocorticoid receptor and heat shock protein 70 in aged Japanese quail exposed to acute heat stress. Poultry Science, 90 (7). pp. 1427-1434. ISSN 0032-5791 10.3382/ps.2011-01403 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description This study aimed to determine the effect of neonatal feed restriction on plasma corticosterone concentration (CORT), hippocampal glucocorticoid receptor (GR) expression, and heat shock protein (Hsp) 70 expression in aged male Japanese quail subjected to acute heat stress. Equal numbers of chicks were subjected to either ad libitum feeding (AL) or 60% feed restriction on d 4, 5, and 6 (FR). At 21 (young) and 270 (aged) d of age, birds were exposed to 43 ± 1°C for 1 h. Blood and hippocampus samples were collected to determine CORT and Hsp 70 and GR expressions before heat stress and following 1 h of heat stress, 1 h of post-heat stress recovery, and 2 h of post-heat stress recovery. With the use of real-time PCR and enzyme immunoassay, we examined the hippocampal expression of GR and Hsp 70 and CORT. The GR expression of the young birds increased following heat stress and remained consistent throughout the period of recovery. Conversely, no significant changes were noted on GR expression of aged birds. Although both young and aged birds had similar CORT before and during heat stress, the latter exhibited greater values following 1 and 2 h of recovery. Within the young group, feeding regimens had no significant effect on Hsp 70 expression. However, neonatal feed restriction improved Hsp 70 expression in aged birds. Neonatal feed restriction, compared with the AL group, resulted in higher CORT on d 21 but the converse was noted on d 270. Neonatal feed restriction appears to set a robust reactive hypothalamo-pituitary-adrenal response allowing the development of adaptive, healthy, and resilient phenotypes in aged quail as measured by a higher hippocampal Hsp 70 expression along with lower CORT.
format Article
author A. F., Soleimani
Idrus, Zulkifli
Omar, Abdul Rahman
Abdul Rahim, Raha
spellingShingle A. F., Soleimani
Idrus, Zulkifli
Omar, Abdul Rahman
Abdul Rahim, Raha
Neonatal feed restriction modulates circulating levels of corticosterone and expression of glucocorticoid receptor and heat shock protein 70 in aged Japanese quail exposed to acute heat stress.
author_facet A. F., Soleimani
Idrus, Zulkifli
Omar, Abdul Rahman
Abdul Rahim, Raha
author_sort A. F., Soleimani
title Neonatal feed restriction modulates circulating levels of corticosterone and expression of glucocorticoid receptor and heat shock protein 70 in aged Japanese quail exposed to acute heat stress.
title_short Neonatal feed restriction modulates circulating levels of corticosterone and expression of glucocorticoid receptor and heat shock protein 70 in aged Japanese quail exposed to acute heat stress.
title_full Neonatal feed restriction modulates circulating levels of corticosterone and expression of glucocorticoid receptor and heat shock protein 70 in aged Japanese quail exposed to acute heat stress.
title_fullStr Neonatal feed restriction modulates circulating levels of corticosterone and expression of glucocorticoid receptor and heat shock protein 70 in aged Japanese quail exposed to acute heat stress.
title_full_unstemmed Neonatal feed restriction modulates circulating levels of corticosterone and expression of glucocorticoid receptor and heat shock protein 70 in aged Japanese quail exposed to acute heat stress.
title_sort neonatal feed restriction modulates circulating levels of corticosterone and expression of glucocorticoid receptor and heat shock protein 70 in aged japanese quail exposed to acute heat stress.
publisher Poultry Science Association Inc.
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/25337/1/Neonatal%20feed%20restriction%20modulates%20circulating%20levels%20of%20corticosterone%20and%20expression%20of%20glucocorticoid%20receptor%20and%20heat%20shock%20protein%2070%20in%20aged%20Japanese%20quail%20exposed%20to%20acute%20heat%20stress.pdf
http://psasir.upm.edu.my/id/eprint/25337/
_version_ 1643828631834722304
score 13.211869

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