Staff View: Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein.

Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein.

Studies have shown that the VP22 gene of Marek’s Disease Virus type-1 (MDV-1) has the property of movement between cells from the original cell of expression into the neighboring cells. The ability to facilitate the spreading of the linked proteins was used to improve the potency of the constructed...

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Main Authors:	Moeini, Hassan, Omar, Abdul Rahman, Abdul Rahim, Raha, Yusoff, Khatijah
Format:	Article
Language:	English English
Published:	BioMed Central 2011
Online Access:	http://psasir.upm.edu.my/id/eprint/25332/1/Improving%20the%20potency%20of%20DNA%20vaccine%20against%20Chicken%20Anemia%20Virus.pdf http://psasir.upm.edu.my/id/eprint/25332/
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spelling	my.upm.eprints.253322015-10-16T07:27:19Z http://psasir.upm.edu.my/id/eprint/25332/ Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein. Moeini, Hassan Omar, Abdul Rahman Abdul Rahim, Raha Yusoff, Khatijah Studies have shown that the VP22 gene of Marek’s Disease Virus type-1 (MDV-1) has the property of movement between cells from the original cell of expression into the neighboring cells. The ability to facilitate the spreading of the linked proteins was used to improve the potency of the constructed DNA vaccines against chicken anemia virus (CAV). The VP1 and VP2 genes of CAV isolate SMSC-1 were amplified and inserted into eukaryotic co-expression vector, pBudCE4.1 to construct pBudVP2-VP1. We also constructed pBudVP2-VP1/VP22 encoding CAV VP2 and the VP22 of MDV-1 linked to the CAV VP1. In vitro expression of the genes was confirmed by using RT- PCR, Western blot and indirect immunofluorescence. The vaccines were then tested in 2-week-old SPF chickens which were inoculated with the DNA plasmid constructs by the intramuscular route. After in vivo expression studies, immune responses of the immunized chickens were evaluated pre- and post-immunization. Chickens vaccinated with pBudVP2-VP1/VP22 exhibited a significant increase in antibody titers to CAV and also proliferation induction of splenocytes in comparison to the chickens vaccinated with pBudVP2-VP1. Furthermore, the pBudVP2-VP1/VP22-vaccinated group showed higher level of the Th1 cytokines IL-2 and IFN-g. This study showed that MDV-1 VP22 gene is capable of enhancing the potency of DNA vaccine against CAV when fused with the CAV VP1 gene. BioMed Central 2011-03-14 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25332/1/Improving%20the%20potency%20of%20DNA%20vaccine%20against%20Chicken%20Anemia%20Virus.pdf Moeini, Hassan and Omar, Abdul Rahman and Abdul Rahim, Raha and Yusoff, Khatijah (2011) Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein. Virology Journal, 8 (119). pp. 1-9. ISSN 1743-422X 10.1186/1743-422X-8-119 English
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English English
description	Studies have shown that the VP22 gene of Marek’s Disease Virus type-1 (MDV-1) has the property of movement between cells from the original cell of expression into the neighboring cells. The ability to facilitate the spreading of the linked proteins was used to improve the potency of the constructed DNA vaccines against chicken anemia virus (CAV). The VP1 and VP2 genes of CAV isolate SMSC-1 were amplified and inserted into eukaryotic co-expression vector, pBudCE4.1 to construct pBudVP2-VP1. We also constructed pBudVP2-VP1/VP22 encoding CAV VP2 and the VP22 of MDV-1 linked to the CAV VP1. In vitro expression of the genes was confirmed by using RT- PCR, Western blot and indirect immunofluorescence. The vaccines were then tested in 2-week-old SPF chickens which were inoculated with the DNA plasmid constructs by the intramuscular route. After in vivo expression studies, immune responses of the immunized chickens were evaluated pre- and post-immunization. Chickens vaccinated with pBudVP2-VP1/VP22 exhibited a significant increase in antibody titers to CAV and also proliferation induction of splenocytes in comparison to the chickens vaccinated with pBudVP2-VP1. Furthermore, the pBudVP2-VP1/VP22-vaccinated group showed higher level of the Th1 cytokines IL-2 and IFN-g. This study showed that MDV-1 VP22 gene is capable of enhancing the potency of DNA vaccine against CAV when fused with the CAV VP1 gene.
format	Article
author	Moeini, Hassan Omar, Abdul Rahman Abdul Rahim, Raha Yusoff, Khatijah
spellingShingle	Moeini, Hassan Omar, Abdul Rahman Abdul Rahim, Raha Yusoff, Khatijah Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein.
author_facet	Moeini, Hassan Omar, Abdul Rahman Abdul Rahim, Raha Yusoff, Khatijah
author_sort	Moeini, Hassan
title	Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein.
title_short	Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein.
title_full	Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein.
title_fullStr	Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein.
title_full_unstemmed	Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein.
title_sort	improving the potency of dna vaccine against chicken anemia virus (cav) by fusing vp1 protein of cav to marek's disease virus (mdv) type-1 vp22 protein.
publisher	BioMed Central
publishDate	2011
url	http://psasir.upm.edu.my/id/eprint/25332/1/Improving%20the%20potency%20of%20DNA%20vaccine%20against%20Chicken%20Anemia%20Virus.pdf http://psasir.upm.edu.my/id/eprint/25332/
_version_	1643828630357278720
score	13.211869

Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein.

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