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Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV.

In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP...

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Main Authors: Moeini, Hassan, Abdul Rahim, Raha, Omar, Abdul Rahman, Yusoff, Khatijah
Format: Article
Language:English
English
Published: Elsevier 2011
Online Access:http://psasir.upm.edu.my/id/eprint/25331/1/Development%20of%20a%20DNA%20vaccine%20against%20chicken%20anemia%20virus%20by%20using%20a%20bicistronic%20vector%20expressing%20VP1%20and%20VP2%20proteins%20of%20CAV.pdf
http://psasir.upm.edu.my/id/eprint/25331/
http://www.elsevier.com/
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spelling my.upm.eprints.253312015-10-16T07:13:23Z http://psasir.upm.edu.my/id/eprint/25331/ Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV. Moeini, Hassan Abdul Rahim, Raha Omar, Abdul Rahman Yusoff, Khatijah In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines were tested in 2-week-old SPF chickens. Chickens immunized with the DNA-plasmid pBudVP2-VP1 showed positive neutralizing antibody titer against CAV. Furthermore, VP1-specific proliferation induction of splenocytes and also high serum levels of Th1 cytokines, IL-2 and IFN-γ were detected in the pBudVP2-VP1-vaccinated chickens. These results suggest that the recombinant DNA plasmid co-expressing VP1 and VP2 can be used as a potential DNA vaccine against CAV. Elsevier 2011-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25331/1/Development%20of%20a%20DNA%20vaccine%20against%20chicken%20anemia%20virus%20by%20using%20a%20bicistronic%20vector%20expressing%20VP1%20and%20VP2%20proteins%20of%20CAV.pdf Moeini, Hassan and Abdul Rahim, Raha and Omar, Abdul Rahman and Yusoff, Khatijah (2011) Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV. Comparative Immunology, Microbiology and Infectious Diseases, 34 (3). pp. 227-236. ISSN 0147-9571 http://www.elsevier.com/ 10.1016/j.cimid.2010.11.006 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines were tested in 2-week-old SPF chickens. Chickens immunized with the DNA-plasmid pBudVP2-VP1 showed positive neutralizing antibody titer against CAV. Furthermore, VP1-specific proliferation induction of splenocytes and also high serum levels of Th1 cytokines, IL-2 and IFN-γ were detected in the pBudVP2-VP1-vaccinated chickens. These results suggest that the recombinant DNA plasmid co-expressing VP1 and VP2 can be used as a potential DNA vaccine against CAV.
format Article
author Moeini, Hassan
Abdul Rahim, Raha
Omar, Abdul Rahman
Yusoff, Khatijah
spellingShingle Moeini, Hassan
Abdul Rahim, Raha
Omar, Abdul Rahman
Yusoff, Khatijah
Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV.
author_facet Moeini, Hassan
Abdul Rahim, Raha
Omar, Abdul Rahman
Yusoff, Khatijah
author_sort Moeini, Hassan
title Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV.
title_short Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV.
title_full Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV.
title_fullStr Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV.
title_full_unstemmed Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV.
title_sort development of a dna vaccine against chicken anemia virus by using a bicistronic vector expressing vp1 and vp2 proteins of cav.
publisher Elsevier
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/25331/1/Development%20of%20a%20DNA%20vaccine%20against%20chicken%20anemia%20virus%20by%20using%20a%20bicistronic%20vector%20expressing%20VP1%20and%20VP2%20proteins%20of%20CAV.pdf
http://psasir.upm.edu.my/id/eprint/25331/
http://www.elsevier.com/
_version_ 1643828630059483136
score 13.211869

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