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Modeling and optimization of lipase-catalyzed production of succinic acid ester using central composite design analysis.

Esterification of succinic acid with oleyl alcohol catalyzed by immobilized Candida antarctica lipase B (Novozym 435) was investigated in this study. Response surface methodology (RSM) based on a five-level, four-variable central composite design (CCD) was used to model and analyze the reaction. A t...

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Main Authors: Abdul Rahman, Mohd Basyaruddin, Jarmi, Nor Izuan, Chaibakhsh, Naz, Basri, Mahiran
Format: Article
Language:English
English
Published: Springer Link 2011
Online Access:http://psasir.upm.edu.my/id/eprint/25058/1/Modeling%20and%20optimization%20of%20lipase.pdf
http://psasir.upm.edu.my/id/eprint/25058/
http://link.springer.com/
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spelling my.upm.eprints.250582015-10-02T08:03:51Z http://psasir.upm.edu.my/id/eprint/25058/ Modeling and optimization of lipase-catalyzed production of succinic acid ester using central composite design analysis. Abdul Rahman, Mohd Basyaruddin Jarmi, Nor Izuan Chaibakhsh, Naz Basri, Mahiran Esterification of succinic acid with oleyl alcohol catalyzed by immobilized Candida antarctica lipase B (Novozym 435) was investigated in this study. Response surface methodology (RSM) based on a five-level, four-variable central composite design (CCD) was used to model and analyze the reaction. A total of 21 experiments representing different combinations of the four parameters including temperature (35–65°C), time (30–450 min), enzyme amount (20-400 mg), and alcohol:acid molar ratio (1:1-8:1) were generated. A partial cubic equation could accurately model the response surface with a R2 of 0.9853. The effect and interactions of the variables on the ester synthesis were also studied. Temperature was found to be the most significant parameter that influenced the succinate ester synthesis. At the optimal conditions of 41.1°C, 272.8 min, 20 mg enzyme amount and 7.8:1 alcohol:acid molar ratio, the esterification percentage was 85.0%. The model can present a rapid means for estimating the conversion yield of succinate ester within the selected ranges. Springer Link 2011-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25058/1/Modeling%20and%20optimization%20of%20lipase.pdf Abdul Rahman, Mohd Basyaruddin and Jarmi, Nor Izuan and Chaibakhsh, Naz and Basri, Mahiran (2011) Modeling and optimization of lipase-catalyzed production of succinic acid ester using central composite design analysis. Journal of Industrial Microbiology and Technology, 38 (1). pp. 229-234. ISSN 1367-5435 http://link.springer.com/ 10.1007/s10295-010-0817-3 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Esterification of succinic acid with oleyl alcohol catalyzed by immobilized Candida antarctica lipase B (Novozym 435) was investigated in this study. Response surface methodology (RSM) based on a five-level, four-variable central composite design (CCD) was used to model and analyze the reaction. A total of 21 experiments representing different combinations of the four parameters including temperature (35–65°C), time (30–450 min), enzyme amount (20-400 mg), and alcohol:acid molar ratio (1:1-8:1) were generated. A partial cubic equation could accurately model the response surface with a R2 of 0.9853. The effect and interactions of the variables on the ester synthesis were also studied. Temperature was found to be the most significant parameter that influenced the succinate ester synthesis. At the optimal conditions of 41.1°C, 272.8 min, 20 mg enzyme amount and 7.8:1 alcohol:acid molar ratio, the esterification percentage was 85.0%. The model can present a rapid means for estimating the conversion yield of succinate ester within the selected ranges.
format Article
author Abdul Rahman, Mohd Basyaruddin
Jarmi, Nor Izuan
Chaibakhsh, Naz
Basri, Mahiran
spellingShingle Abdul Rahman, Mohd Basyaruddin
Jarmi, Nor Izuan
Chaibakhsh, Naz
Basri, Mahiran
Modeling and optimization of lipase-catalyzed production of succinic acid ester using central composite design analysis.
author_facet Abdul Rahman, Mohd Basyaruddin
Jarmi, Nor Izuan
Chaibakhsh, Naz
Basri, Mahiran
author_sort Abdul Rahman, Mohd Basyaruddin
title Modeling and optimization of lipase-catalyzed production of succinic acid ester using central composite design analysis.
title_short Modeling and optimization of lipase-catalyzed production of succinic acid ester using central composite design analysis.
title_full Modeling and optimization of lipase-catalyzed production of succinic acid ester using central composite design analysis.
title_fullStr Modeling and optimization of lipase-catalyzed production of succinic acid ester using central composite design analysis.
title_full_unstemmed Modeling and optimization of lipase-catalyzed production of succinic acid ester using central composite design analysis.
title_sort modeling and optimization of lipase-catalyzed production of succinic acid ester using central composite design analysis.
publisher Springer Link
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/25058/1/Modeling%20and%20optimization%20of%20lipase.pdf
http://psasir.upm.edu.my/id/eprint/25058/
http://link.springer.com/
_version_ 1643828545864073216
score 13.211869

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