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Prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus.

Clinical information about genotypically different clones of biofilm-producing Staphylococcus aureus is largely unknown. We examined whether different clones of methicillin-sensitive and methicillin-resistant S. aureus (MSSA and MRSA) differ with respect to staphylococcal microbial surface component...

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Main Authors: Atshan, Salman Sahab, Nor Shamsudin, Mariana, Sekawi, Zamberi, Than, Leslie Thian Lung, Hamat, Rukman Awang, Karunanidhi, A., Ali, AlreshidiMateg, Ghaznavi-Rad, Ehsanollah, Ghasemzadeh-Moghaddam, Hamed, Chong, Pei Pei, Nathan , J.J., Chong , Seng J.S.
Format: Article
Language:English
English
Published: Hindawi Publishing Corporation 2012
Online Access:http://psasir.upm.edu.my/id/eprint/24739/1/Prevalence%20of%20adhesion%20and%20regulation%20of%20biofilm.pdf
http://psasir.upm.edu.my/id/eprint/24739/
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spelling my.upm.eprints.247392015-09-29T01:23:32Z http://psasir.upm.edu.my/id/eprint/24739/ Prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus. Atshan, Salman Sahab Nor Shamsudin, Mariana Sekawi, Zamberi Than, Leslie Thian Lung Hamat, Rukman Awang Karunanidhi, A. Ali, AlreshidiMateg Ghaznavi-Rad, Ehsanollah Ghasemzadeh-Moghaddam, Hamed Chong, Pei Pei Nathan , J.J. Chong , Seng J.S. Clinical information about genotypically different clones of biofilm-producing Staphylococcus aureus is largely unknown. We examined whether different clones of methicillin-sensitive and methicillin-resistant S. aureus (MSSA and MRSA) differ with respect to staphylococcal microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) in biofilm formation. The study used 60 different types of spa and determined the phenotypes, the prevalence of the 13 MSCRAMM, and biofilm genes for each clone. The current investigation was carried out using a modified Congo red agar (MCRA), a microtiter plate assay (MPA), polymerase chain reaction (PCR), and reverse transcriptase polymerase chain reaction (RT-PCR). Clones belonging to the same spa type were found to have similar properties in adheringto thepolystyrene microtiter plate surface. However, their ability to produce slime on MCRA medium was different. PCR experiments showed that 60 clones of MSSA and MRSA were positive for 5 genes (out of 9 MSCRAMM genes). icaADBC genes were found to be present in all the 60 clones tested indicating a high prevalence, and these genes were equally distributed among the clones associated with MSSA and those with MRSA. The prevalence of other MSCRAMM genes among MSSA and MRSA clones was found to be variable. MRSA and MSSA gene expression (MSCRAMM and icaADBC) was confirmed by RT-PCR. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24739/1/Prevalence%20of%20adhesion%20and%20regulation%20of%20biofilm.pdf Atshan, Salman Sahab and Nor Shamsudin, Mariana and Sekawi, Zamberi and Than, Leslie Thian Lung and Hamat, Rukman Awang and Karunanidhi, A. and Ali, AlreshidiMateg and Ghaznavi-Rad, Ehsanollah and Ghasemzadeh-Moghaddam, Hamed and Chong, Pei Pei and Nathan , J.J. and Chong , Seng J.S. (2012) Prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus. Journal of Biomedicine and Biotechnology, 2012 (976972). pp. 1-10. ISSN 1110-7243; ESSN:1110-7251 http://www.hindawi.com/ 10.1155/2012/976972 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Clinical information about genotypically different clones of biofilm-producing Staphylococcus aureus is largely unknown. We examined whether different clones of methicillin-sensitive and methicillin-resistant S. aureus (MSSA and MRSA) differ with respect to staphylococcal microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) in biofilm formation. The study used 60 different types of spa and determined the phenotypes, the prevalence of the 13 MSCRAMM, and biofilm genes for each clone. The current investigation was carried out using a modified Congo red agar (MCRA), a microtiter plate assay (MPA), polymerase chain reaction (PCR), and reverse transcriptase polymerase chain reaction (RT-PCR). Clones belonging to the same spa type were found to have similar properties in adheringto thepolystyrene microtiter plate surface. However, their ability to produce slime on MCRA medium was different. PCR experiments showed that 60 clones of MSSA and MRSA were positive for 5 genes (out of 9 MSCRAMM genes). icaADBC genes were found to be present in all the 60 clones tested indicating a high prevalence, and these genes were equally distributed among the clones associated with MSSA and those with MRSA. The prevalence of other MSCRAMM genes among MSSA and MRSA clones was found to be variable. MRSA and MSSA gene expression (MSCRAMM and icaADBC) was confirmed by RT-PCR.
format Article
author Atshan, Salman Sahab
Nor Shamsudin, Mariana
Sekawi, Zamberi
Than, Leslie Thian Lung
Hamat, Rukman Awang
Karunanidhi, A.
Ali, AlreshidiMateg
Ghaznavi-Rad, Ehsanollah
Ghasemzadeh-Moghaddam, Hamed
Chong, Pei Pei
Nathan , J.J.
Chong , Seng J.S.
spellingShingle Atshan, Salman Sahab
Nor Shamsudin, Mariana
Sekawi, Zamberi
Than, Leslie Thian Lung
Hamat, Rukman Awang
Karunanidhi, A.
Ali, AlreshidiMateg
Ghaznavi-Rad, Ehsanollah
Ghasemzadeh-Moghaddam, Hamed
Chong, Pei Pei
Nathan , J.J.
Chong , Seng J.S.
Prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus.
author_facet Atshan, Salman Sahab
Nor Shamsudin, Mariana
Sekawi, Zamberi
Than, Leslie Thian Lung
Hamat, Rukman Awang
Karunanidhi, A.
Ali, AlreshidiMateg
Ghaznavi-Rad, Ehsanollah
Ghasemzadeh-Moghaddam, Hamed
Chong, Pei Pei
Nathan , J.J.
Chong , Seng J.S.
author_sort Atshan, Salman Sahab
title Prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus.
title_short Prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus.
title_full Prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus.
title_fullStr Prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus.
title_full_unstemmed Prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus.
title_sort prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus.
publisher Hindawi Publishing Corporation
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/24739/1/Prevalence%20of%20adhesion%20and%20regulation%20of%20biofilm.pdf
http://psasir.upm.edu.my/id/eprint/24739/
http://www.hindawi.com/
_version_ 1643828451359064064
score 13.211869

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