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Bauhinia purpurea leaves' extracts exhibited in vitro antiproliferative and antioxidant activities

The antiproliferative and antioxidant activities of various extracts of the leaves ofBauhinia purpurea were studied using in vitro standard assays. The aqueous and chloroform extracts successfully inhibited the proliferation of all cancer cells while the methanol extract inhibited the proliferation...

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Main Authors: Zakaria, Zainul Amiruddin, Rofiee, Mohd Salleh, Teh, Lay Kek, Salleh, Mohd Zaki, Sulaiman, Mohd Roslan, Abdullah, Muhammad Nazrul Hakim
Format: Article
Language:English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24726/1/article1380711158_Zakaria%20et%20al.pdf
http://psasir.upm.edu.my/id/eprint/24726/
http://www.academicjournals.org/journal/AJB/article-abstract/78ECC9122462
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spelling my.upm.eprints.247262016-04-27T01:56:35Z http://psasir.upm.edu.my/id/eprint/24726/ Bauhinia purpurea leaves' extracts exhibited in vitro antiproliferative and antioxidant activities Zakaria, Zainul Amiruddin Rofiee, Mohd Salleh Teh, Lay Kek Salleh, Mohd Zaki Sulaiman, Mohd Roslan Abdullah, Muhammad Nazrul Hakim The antiproliferative and antioxidant activities of various extracts of the leaves ofBauhinia purpurea were studied using in vitro standard assays. The aqueous and chloroform extracts successfully inhibited the proliferation of all cancer cells while the methanol extract inhibited the proliferation of all cells except the CEMss cells when assessed using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. The aqueous extract was effective against MCF-7 (IC50 ≈ 9µg/ml), MDA-MB 231 (IC50 ≈ 17 µg/ml) and Caov-3 (IC50 ≈ 16 µg/ml); the chloroform extract was highly effective against the CEMss (IC50 ≈ 18 µg/ml) and HeLa (IC50 ≈ 21 µg/ml); and the methanol extract was highly effective only against the HL-60 (≈ 12 µg/ml) cell lines. Interestingly, all extracts did not inhibit the proliferation of 3T3 cells suggesting their non-cytotoxic properties. The aqueous and methanol, but not chloroform, extracts of B. purpurea (20, 100 and 500 mg/ml) exhibited concentration-dependent antioxidant activity only in the superoxide scavenging assay, but low to moderate activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, which could be associated with their total phenolic contents. In conclusion, the B. purpurea leaf possesses potential antiproliferative and concentration-dependent antioxidant activities. Purification and determination of active compounds are required for further study. Academic Journals 2011-01-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24726/1/article1380711158_Zakaria%20et%20al.pdf Zakaria, Zainul Amiruddin and Rofiee, Mohd Salleh and Teh, Lay Kek and Salleh, Mohd Zaki and Sulaiman, Mohd Roslan and Abdullah, Muhammad Nazrul Hakim (2011) Bauhinia purpurea leaves' extracts exhibited in vitro antiproliferative and antioxidant activities. African Journal of Biotechnology, 10 (1). art. no. 78ECC9122462. pp. 65-74. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/78ECC9122462
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The antiproliferative and antioxidant activities of various extracts of the leaves ofBauhinia purpurea were studied using in vitro standard assays. The aqueous and chloroform extracts successfully inhibited the proliferation of all cancer cells while the methanol extract inhibited the proliferation of all cells except the CEMss cells when assessed using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. The aqueous extract was effective against MCF-7 (IC50 ≈ 9µg/ml), MDA-MB 231 (IC50 ≈ 17 µg/ml) and Caov-3 (IC50 ≈ 16 µg/ml); the chloroform extract was highly effective against the CEMss (IC50 ≈ 18 µg/ml) and HeLa (IC50 ≈ 21 µg/ml); and the methanol extract was highly effective only against the HL-60 (≈ 12 µg/ml) cell lines. Interestingly, all extracts did not inhibit the proliferation of 3T3 cells suggesting their non-cytotoxic properties. The aqueous and methanol, but not chloroform, extracts of B. purpurea (20, 100 and 500 mg/ml) exhibited concentration-dependent antioxidant activity only in the superoxide scavenging assay, but low to moderate activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, which could be associated with their total phenolic contents. In conclusion, the B. purpurea leaf possesses potential antiproliferative and concentration-dependent antioxidant activities. Purification and determination of active compounds are required for further study.
format Article
author Zakaria, Zainul Amiruddin
Rofiee, Mohd Salleh
Teh, Lay Kek
Salleh, Mohd Zaki
Sulaiman, Mohd Roslan
Abdullah, Muhammad Nazrul Hakim
spellingShingle Zakaria, Zainul Amiruddin
Rofiee, Mohd Salleh
Teh, Lay Kek
Salleh, Mohd Zaki
Sulaiman, Mohd Roslan
Abdullah, Muhammad Nazrul Hakim
Bauhinia purpurea leaves' extracts exhibited in vitro antiproliferative and antioxidant activities
author_facet Zakaria, Zainul Amiruddin
Rofiee, Mohd Salleh
Teh, Lay Kek
Salleh, Mohd Zaki
Sulaiman, Mohd Roslan
Abdullah, Muhammad Nazrul Hakim
author_sort Zakaria, Zainul Amiruddin
title Bauhinia purpurea leaves' extracts exhibited in vitro antiproliferative and antioxidant activities
title_short Bauhinia purpurea leaves' extracts exhibited in vitro antiproliferative and antioxidant activities
title_full Bauhinia purpurea leaves' extracts exhibited in vitro antiproliferative and antioxidant activities
title_fullStr Bauhinia purpurea leaves' extracts exhibited in vitro antiproliferative and antioxidant activities
title_full_unstemmed Bauhinia purpurea leaves' extracts exhibited in vitro antiproliferative and antioxidant activities
title_sort bauhinia purpurea leaves' extracts exhibited in vitro antiproliferative and antioxidant activities
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24726/1/article1380711158_Zakaria%20et%20al.pdf
http://psasir.upm.edu.my/id/eprint/24726/
http://www.academicjournals.org/journal/AJB/article-abstract/78ECC9122462
_version_ 1643828447434244096
score 13.222552

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