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Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli

Despite the frequent isolation of extended-spectrum beta-lactamase producing Escherichia coli from extra-intestinal infections (ESBL-ExPEC), the virulent properties or the antibiotic resistance mechanisms or its phylogenetics is rarely studied in Malaysia. In the present investigation, we characteri...

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Main Authors: Al-Hetar, Khadega Yahya Abdullah, Sekawi, Zamberi, Shamsudin, Mariana Nor, Neela, Vasantha Kumari
Format: Article
Language:English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24703/1/24703.pdf
http://psasir.upm.edu.my/id/eprint/24703/
http://www.academicjournals.org/journal/AJMR/article-abstract/5E0281D27330
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spelling my.upm.eprints.247032017-11-17T02:05:02Z http://psasir.upm.edu.my/id/eprint/24703/ Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli Al-Hetar, Khadega Yahya Abdullah Sekawi, Zamberi Shamsudin, Mariana Nor Neela, Vasantha Kumari Despite the frequent isolation of extended-spectrum beta-lactamase producing Escherichia coli from extra-intestinal infections (ESBL-ExPEC), the virulent properties or the antibiotic resistance mechanisms or its phylogenetics is rarely studied in Malaysia. In the present investigation, we characterized 95 clinical isolates of ESBL-ExPEC collected over a one year period time from a tertiary hospital through Clermont dichotomous phylogenetic grouping, virulent gene profiling and ESBL production mechanisms. The highest frequency of E. coli isolation was observed among urine (40% / n=38), while the lowest was among tissue and peritoneal dialyses fluid (1.05% / n= 1). Phylogenetic grouping revealed that 42 (44.21%) isolates belonged to group D, while 26 (27.36%), 14(14.73%) and 13 (13.68) belonged to A, B2 and B1. All isolates were multi drug resistant, whereby 58.9% harboured CTX-M-1 and 22.10% CTX-M-9. None of the isolates carried CTX-M-2 or CTX-M-8 clusters. Eight (7.6%) isolates were positive for blaTEM-1 and two (1.9%) produced SHV-12 beta-lactamase. The CTX-M-9 cluster was found to be significantly (P=0.005) associated with pathogenic group. Majority of the isolates (48.42%) carried class 1 integrons, while 3 (3.15%) isolates showed positive for class 2-encodedintI2 integrase and no signal for class 3 integron. Virulence factors profiling identified iutA56 (58.94%) and kpsMII33 (34.73%) as frequent pathogenic determinants. Virulence studies on Caenorhabditis elegans showed that antibiotic susceptible E. coli strains have short life span than pathogenic or commensal ones. In conclusion, it is found that commensals are becoming multiple drug resistant and carries several virulent genes which warn the spread of drug resistance or virulent factors to the normal flora. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24703/1/24703.pdf Al-Hetar, Khadega Yahya Abdullah and Sekawi, Zamberi and Shamsudin, Mariana Nor and Neela, Vasantha Kumari (2011) Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli. African Journal of Microbiology Research, 5 (31). art. no. 5E0281D27330. pp. 5662-5668. ISSN 1996-0808 http://www.academicjournals.org/journal/AJMR/article-abstract/5E0281D27330
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Despite the frequent isolation of extended-spectrum beta-lactamase producing Escherichia coli from extra-intestinal infections (ESBL-ExPEC), the virulent properties or the antibiotic resistance mechanisms or its phylogenetics is rarely studied in Malaysia. In the present investigation, we characterized 95 clinical isolates of ESBL-ExPEC collected over a one year period time from a tertiary hospital through Clermont dichotomous phylogenetic grouping, virulent gene profiling and ESBL production mechanisms. The highest frequency of E. coli isolation was observed among urine (40% / n=38), while the lowest was among tissue and peritoneal dialyses fluid (1.05% / n= 1). Phylogenetic grouping revealed that 42 (44.21%) isolates belonged to group D, while 26 (27.36%), 14(14.73%) and 13 (13.68) belonged to A, B2 and B1. All isolates were multi drug resistant, whereby 58.9% harboured CTX-M-1 and 22.10% CTX-M-9. None of the isolates carried CTX-M-2 or CTX-M-8 clusters. Eight (7.6%) isolates were positive for blaTEM-1 and two (1.9%) produced SHV-12 beta-lactamase. The CTX-M-9 cluster was found to be significantly (P=0.005) associated with pathogenic group. Majority of the isolates (48.42%) carried class 1 integrons, while 3 (3.15%) isolates showed positive for class 2-encodedintI2 integrase and no signal for class 3 integron. Virulence factors profiling identified iutA56 (58.94%) and kpsMII33 (34.73%) as frequent pathogenic determinants. Virulence studies on Caenorhabditis elegans showed that antibiotic susceptible E. coli strains have short life span than pathogenic or commensal ones. In conclusion, it is found that commensals are becoming multiple drug resistant and carries several virulent genes which warn the spread of drug resistance or virulent factors to the normal flora.
format Article
author Al-Hetar, Khadega Yahya Abdullah
Sekawi, Zamberi
Shamsudin, Mariana Nor
Neela, Vasantha Kumari
spellingShingle Al-Hetar, Khadega Yahya Abdullah
Sekawi, Zamberi
Shamsudin, Mariana Nor
Neela, Vasantha Kumari
Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli
author_facet Al-Hetar, Khadega Yahya Abdullah
Sekawi, Zamberi
Shamsudin, Mariana Nor
Neela, Vasantha Kumari
author_sort Al-Hetar, Khadega Yahya Abdullah
title Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli
title_short Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli
title_full Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli
title_fullStr Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli
title_full_unstemmed Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli
title_sort molecular characterization of extended-spectrum beta-lactimase (esbl) producing extra-intestinal pathogenic escherichia coli
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24703/1/24703.pdf
http://psasir.upm.edu.my/id/eprint/24703/
http://www.academicjournals.org/journal/AJMR/article-abstract/5E0281D27330
_version_ 1643828440547196928
score 13.211869

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