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Identification of local clinical Candida isolates using CHROMagar Candida TM as a primary identification method for various Candida species.

The objective of our study was to study the effectiveness of CHROMagar Candida TM as the primary identification method for various clinical Candida isolates, other than the three suggested species by the manufacturer. We studied 34 clinical isolates which were isolated from patients in a local teach...

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Main Authors: Madhavan, P., Jamal, Farida Fatema @ Farida, Chong, Pei Pei, Ng, Kee Peng
Format: Article
Language:English
English
Published: Malaysian Society of Parasitology and Tropical Medicine 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24666/1/Identification%20of%20local%20clinical%20Candida%20isolates%20using%20CHROMagar%20Candida%20TM%20as%20a%20primary%20identification%20method%20for%20various%20Candida%20species.pdf
http://psasir.upm.edu.my/id/eprint/24666/
http://msptm.org/
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spelling my.upm.eprints.246662015-10-20T00:06:26Z http://psasir.upm.edu.my/id/eprint/24666/ Identification of local clinical Candida isolates using CHROMagar Candida TM as a primary identification method for various Candida species. Madhavan, P. Jamal, Farida Fatema @ Farida Chong, Pei Pei Ng, Kee Peng The objective of our study was to study the effectiveness of CHROMagar Candida TM as the primary identification method for various clinical Candida isolates, other than the three suggested species by the manufacturer. We studied 34 clinical isolates which were isolated from patients in a local teaching hospital and 7 ATCC strains. These strains were first cultured in Sabouraud dextrose broth (SDB) for 36 hours at 35°C, then on CHROMagar plates at 30°C, 35°C and 37°C. The sensitivity of this agar to identify Candida albicans, Candida dubliniensis, Candida tropicalis, Candida glabrata, Candida rugosa, Candida krusei and Candida parapsilosis ranged between 25 and 100% at 30°C, 14% and 100% at 35°C, 56% and 100% at 37°C. The specificity of this agar was 100% at 30°C, between 97% and 100% at 35°C, 92% and 100% at 37°C. The efficiency of this agar ranged between 88 and 100% at 30°C, 83% and 100% at 35°C, 88% and 100% at 37°C. Each species also gave rise to a variety of colony colours ranging from pink to green to blue of different colony characteristics. Therefore, the chromogenic agar was found to be useful in our study for identifying clinical Candida isolates. Malaysian Society of Parasitology and Tropical Medicine 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24666/1/Identification%20of%20local%20clinical%20Candida%20isolates%20using%20CHROMagar%20Candida%20TM%20as%20a%20primary%20identification%20method%20for%20various%20Candida%20species.pdf Madhavan, P. and Jamal, Farida Fatema @ Farida and Chong, Pei Pei and Ng, Kee Peng (2011) Identification of local clinical Candida isolates using CHROMagar Candida TM as a primary identification method for various Candida species. Tropical Biomedicine, 28 (2). pp. 269-274. ISSN 0127-5720 http://msptm.org/ English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The objective of our study was to study the effectiveness of CHROMagar Candida TM as the primary identification method for various clinical Candida isolates, other than the three suggested species by the manufacturer. We studied 34 clinical isolates which were isolated from patients in a local teaching hospital and 7 ATCC strains. These strains were first cultured in Sabouraud dextrose broth (SDB) for 36 hours at 35°C, then on CHROMagar plates at 30°C, 35°C and 37°C. The sensitivity of this agar to identify Candida albicans, Candida dubliniensis, Candida tropicalis, Candida glabrata, Candida rugosa, Candida krusei and Candida parapsilosis ranged between 25 and 100% at 30°C, 14% and 100% at 35°C, 56% and 100% at 37°C. The specificity of this agar was 100% at 30°C, between 97% and 100% at 35°C, 92% and 100% at 37°C. The efficiency of this agar ranged between 88 and 100% at 30°C, 83% and 100% at 35°C, 88% and 100% at 37°C. Each species also gave rise to a variety of colony colours ranging from pink to green to blue of different colony characteristics. Therefore, the chromogenic agar was found to be useful in our study for identifying clinical Candida isolates.
format Article
author Madhavan, P.
Jamal, Farida Fatema @ Farida
Chong, Pei Pei
Ng, Kee Peng
spellingShingle Madhavan, P.
Jamal, Farida Fatema @ Farida
Chong, Pei Pei
Ng, Kee Peng
Identification of local clinical Candida isolates using CHROMagar Candida TM as a primary identification method for various Candida species.
author_facet Madhavan, P.
Jamal, Farida Fatema @ Farida
Chong, Pei Pei
Ng, Kee Peng
author_sort Madhavan, P.
title Identification of local clinical Candida isolates using CHROMagar Candida TM as a primary identification method for various Candida species.
title_short Identification of local clinical Candida isolates using CHROMagar Candida TM as a primary identification method for various Candida species.
title_full Identification of local clinical Candida isolates using CHROMagar Candida TM as a primary identification method for various Candida species.
title_fullStr Identification of local clinical Candida isolates using CHROMagar Candida TM as a primary identification method for various Candida species.
title_full_unstemmed Identification of local clinical Candida isolates using CHROMagar Candida TM as a primary identification method for various Candida species.
title_sort identification of local clinical candida isolates using chromagar candida tm as a primary identification method for various candida species.
publisher Malaysian Society of Parasitology and Tropical Medicine
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24666/1/Identification%20of%20local%20clinical%20Candida%20isolates%20using%20CHROMagar%20Candida%20TM%20as%20a%20primary%20identification%20method%20for%20various%20Candida%20species.pdf
http://psasir.upm.edu.my/id/eprint/24666/
http://msptm.org/
_version_ 1643828429494157312
score 13.211869

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