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Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia.

The usefulness of mec-associated dru typing in the epidemiological analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Malaysia was investigated and compared with pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and spa and SCCmec typing. The isolate...

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Main Authors: Ghaznavi-Rad, Ehsanollah, Goering, R. V., Shamsudin, Mariana Nor, Weng, Poh Leng, Sekawi, Zamberi, Tavakol, M., Van Belkum, Alex, Neela, Vasantha Kumari
Format: Article
Language:English
English
Published: Springer Verlag 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24564/1/Mec.pdf
http://psasir.upm.edu.my/id/eprint/24564/
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spelling my.upm.eprints.245642015-11-26T08:42:14Z http://psasir.upm.edu.my/id/eprint/24564/ Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia. Ghaznavi-Rad, Ehsanollah Goering, R. V. Shamsudin, Mariana Nor Weng, Poh Leng Sekawi, Zamberi Tavakol, M. Van Belkum, Alex Neela, Vasantha Kumari The usefulness of mec-associated dru typing in the epidemiological analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Malaysia was investigated and compared with pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and spa and SCCmec typing. The isolates studied included all MRSA types in Malaysia. Multilocus sequence type ST188 and ST1 isolates were highly clonal by all typing methods. However, the dru typing of ST239 isolates produced the clearest discrimination between SCCmec IIIa and III isolates, yielding more subtypes than any other method. Evaluation of the discriminatory power for each method identified dru typing and PFGE as the most discriminatory, with Simpson’s index of diversity (SID) values over 89%, including an isolate which was non-typeable by spa, but dru-typed as dt13j. The discriminatory ability of dru typing, especially with closely related MRSA ST239 strains (e.g., Brazilian and Hungarian), underscores its utility as a tool for the epidemiological investigation of MRSA. Springer Verlag 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24564/1/Mec.pdf Ghaznavi-Rad, Ehsanollah and Goering, R. V. and Shamsudin, Mariana Nor and Weng, Poh Leng and Sekawi, Zamberi and Tavakol, M. and Van Belkum, Alex and Neela, Vasantha Kumari (2011) Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia. European Journal of Clinical Microbiology & Infectious Diseases, 30 (11). pp. 1365-1369. ISSN 0934-9723; ESSN: 1435-4373 10.1007/s10096-011-1230-1 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The usefulness of mec-associated dru typing in the epidemiological analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Malaysia was investigated and compared with pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and spa and SCCmec typing. The isolates studied included all MRSA types in Malaysia. Multilocus sequence type ST188 and ST1 isolates were highly clonal by all typing methods. However, the dru typing of ST239 isolates produced the clearest discrimination between SCCmec IIIa and III isolates, yielding more subtypes than any other method. Evaluation of the discriminatory power for each method identified dru typing and PFGE as the most discriminatory, with Simpson’s index of diversity (SID) values over 89%, including an isolate which was non-typeable by spa, but dru-typed as dt13j. The discriminatory ability of dru typing, especially with closely related MRSA ST239 strains (e.g., Brazilian and Hungarian), underscores its utility as a tool for the epidemiological investigation of MRSA.
format Article
author Ghaznavi-Rad, Ehsanollah
Goering, R. V.
Shamsudin, Mariana Nor
Weng, Poh Leng
Sekawi, Zamberi
Tavakol, M.
Van Belkum, Alex
Neela, Vasantha Kumari
spellingShingle Ghaznavi-Rad, Ehsanollah
Goering, R. V.
Shamsudin, Mariana Nor
Weng, Poh Leng
Sekawi, Zamberi
Tavakol, M.
Van Belkum, Alex
Neela, Vasantha Kumari
Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia.
author_facet Ghaznavi-Rad, Ehsanollah
Goering, R. V.
Shamsudin, Mariana Nor
Weng, Poh Leng
Sekawi, Zamberi
Tavakol, M.
Van Belkum, Alex
Neela, Vasantha Kumari
author_sort Ghaznavi-Rad, Ehsanollah
title Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia.
title_short Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia.
title_full Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia.
title_fullStr Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia.
title_full_unstemmed Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia.
title_sort mec-associated dru typing in the epidemiological analysis of st239 mrsa in malaysia.
publisher Springer Verlag
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24564/1/Mec.pdf
http://psasir.upm.edu.my/id/eprint/24564/
_version_ 1643828397575503872
score 13.222552

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