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Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice.

OBJECTIVE: Azadirachta indica (Neem) has been used traditionally for many centuries. Some impressive therapeutic qualities have been discovered. However, the therapeutic effect of neem leaf extract in 4T1 breast cancer has not been documented. The purpose of the present study is to investigate the...

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Main Authors: Othman, Fauziah, Motalleb, Gholamreza, Lam, Sally Tsuey, Rahmat, Asmah, Fakurazi, Sharida, Chong, Pei Pei
Format: Article
Language:English
Published: Royan Institute (ACECR), Tehran 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24519/
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spelling my.upm.eprints.245192014-03-24T07:21:34Z http://psasir.upm.edu.my/id/eprint/24519/ Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice. Othman, Fauziah Motalleb, Gholamreza Lam, Sally Tsuey Rahmat, Asmah Fakurazi, Sharida Chong, Pei Pei OBJECTIVE: Azadirachta indica (Neem) has been used traditionally for many centuries. Some impressive therapeutic qualities have been discovered. However, the therapeutic effect of neem leaf extract in 4T1 breast cancer has not been documented. The purpose of the present study is to investigate the therapeutic effect of ethanolic Neem leaf extract in an in vivo 4T1 breast cancer model in mice. MATERIALS AND METHODS: A total of 84 female BALB/c mice were divided randomly into 7 groups (3 non-cancerous groups and 4 cancerous groups) consisting of 12 mice per group. The 3 non-cancerous groups were normal mice treated with 0.5% of Tween 20 in phosphate buffer saline (PBS) (NC), 250 mg/kg Neem (N250) or 500 mg/kg Neem (N500). The 4 cancerous groups were; cancer controls treated with 0.5% of Tween 20 in PBS (CC), and cancerous mice treated with 0.5 µg/mL tamoxifen citrate (CT), 250 mg/kg Neem leaf extract (CN 250) or 500 mg/kg Neem leaf extract (CN 500). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were used to evaluate apoptosis (cell death) in the breast cancer tissues. SPSS software, version 14 was used for statistical analysis. Statistical significance was defined as p≤0.05. Non parametric analysis of variance (ANOVA) was performed with the Kruskal Wallis test for the TUNEL assays. Parametric data among the groups was compared using ANOVA. RESULTS: TUNEL assays showed that the CN 250 and CN 500 groups had a higher incidence of apoptosis compared with the cancer controls. CONCLUSION: The findings showed that neem leaf extract induces apoptosis in 4T1 breast cancer BALB/c mice. Royan Institute (ACECR), Tehran 2011 Article PeerReviewed Othman, Fauziah and Motalleb, Gholamreza and Lam, Sally Tsuey and Rahmat, Asmah and Fakurazi, Sharida and Chong, Pei Pei (2011) Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice. Cell Journal, 13 (2). pp. 107-116. ISSN 2228-5806; ESSN: 2228-5814 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description OBJECTIVE: Azadirachta indica (Neem) has been used traditionally for many centuries. Some impressive therapeutic qualities have been discovered. However, the therapeutic effect of neem leaf extract in 4T1 breast cancer has not been documented. The purpose of the present study is to investigate the therapeutic effect of ethanolic Neem leaf extract in an in vivo 4T1 breast cancer model in mice. MATERIALS AND METHODS: A total of 84 female BALB/c mice were divided randomly into 7 groups (3 non-cancerous groups and 4 cancerous groups) consisting of 12 mice per group. The 3 non-cancerous groups were normal mice treated with 0.5% of Tween 20 in phosphate buffer saline (PBS) (NC), 250 mg/kg Neem (N250) or 500 mg/kg Neem (N500). The 4 cancerous groups were; cancer controls treated with 0.5% of Tween 20 in PBS (CC), and cancerous mice treated with 0.5 µg/mL tamoxifen citrate (CT), 250 mg/kg Neem leaf extract (CN 250) or 500 mg/kg Neem leaf extract (CN 500). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were used to evaluate apoptosis (cell death) in the breast cancer tissues. SPSS software, version 14 was used for statistical analysis. Statistical significance was defined as p≤0.05. Non parametric analysis of variance (ANOVA) was performed with the Kruskal Wallis test for the TUNEL assays. Parametric data among the groups was compared using ANOVA. RESULTS: TUNEL assays showed that the CN 250 and CN 500 groups had a higher incidence of apoptosis compared with the cancer controls. CONCLUSION: The findings showed that neem leaf extract induces apoptosis in 4T1 breast cancer BALB/c mice.
format Article
author Othman, Fauziah
Motalleb, Gholamreza
Lam, Sally Tsuey
Rahmat, Asmah
Fakurazi, Sharida
Chong, Pei Pei
spellingShingle Othman, Fauziah
Motalleb, Gholamreza
Lam, Sally Tsuey
Rahmat, Asmah
Fakurazi, Sharida
Chong, Pei Pei
Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice.
author_facet Othman, Fauziah
Motalleb, Gholamreza
Lam, Sally Tsuey
Rahmat, Asmah
Fakurazi, Sharida
Chong, Pei Pei
author_sort Othman, Fauziah
title Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice.
title_short Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice.
title_full Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice.
title_fullStr Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice.
title_full_unstemmed Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice.
title_sort extract of azadirachta indica (neem) leaf induces apoptosis in 4t1 breast cancer balb/c mice.
publisher Royan Institute (ACECR), Tehran
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24519/
_version_ 1643828384580501504
score 13.211869

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