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Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines.

Nigella sativa has been used for centuries in Asia, Middle East and Africa to promote health and fight diseases. In this study, the anti-cancer effects of thymoquinone rich fraction (TQRF) extracted from N. sativa seeds using supercritical fluid extraction (SFE) system and commercially available thy...

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Main Authors: Ismail, Norsharina, Ismail, Maznah, Al-Absi, Aied, Al-Naqeeb, Ghanya
Format: Article
Language:English
English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24493/1/Thymoquinone%20rich%20fraction%20from%20Nigella%20sativa%20and%20thymoquinone%20are%20cytotoxic%20towards%20colon%20and%20leukemic%20carcinoma%20cell%20lines.pdf
http://psasir.upm.edu.my/id/eprint/24493/
http://www.academicjournals.org/journal/JMPR
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spelling my.upm.eprints.244932015-09-22T07:44:33Z http://psasir.upm.edu.my/id/eprint/24493/ Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines. Ismail, Norsharina Ismail, Maznah Al-Absi, Aied Al-Naqeeb, Ghanya Nigella sativa has been used for centuries in Asia, Middle East and Africa to promote health and fight diseases. In this study, the anti-cancer effects of thymoquinone rich fraction (TQRF) extracted from N. sativa seeds using supercritical fluid extraction (SFE) system and commercially available thymoquinone (TQ) on colon cancer (HT29), lymphoblastic leukemia (CEMSS) and promyelocytic leukemia (HL60) cells lines were investigated. The concentration that gave 50% inhibition of cell viability (IC50) of HT29, CEMSS and HL60 cells treated with TQRF were 400, 350 and 250 µg/ml, respectively. Meanwhile, the IC50 of TQ was 8, 5 and 3 µg/ml, respectively. Cell cycle analysis shows the increment of apoptosis in a time-dependent manner. However, both TQRF and TQ were not able to arrest the cell cycle phases of the cells. Apoptosis was the main mode of HT29, CEMSS and HL60 cells death induced by both TQRF and TQ. Our findings support the potential use of TQRF and TQ for the treatment of colon cancer and leukemia. Academic Journals 2011-08-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24493/1/Thymoquinone%20rich%20fraction%20from%20Nigella%20sativa%20and%20thymoquinone%20are%20cytotoxic%20towards%20colon%20and%20leukemic%20carcinoma%20cell%20lines.pdf Ismail, Norsharina and Ismail, Maznah and Al-Absi, Aied and Al-Naqeeb, Ghanya (2011) Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines. Journal of Medicinal Plants Research, 5 (15). pp. 3359-3366. ISSN 1996-0875 http://www.academicjournals.org/journal/JMPR English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Nigella sativa has been used for centuries in Asia, Middle East and Africa to promote health and fight diseases. In this study, the anti-cancer effects of thymoquinone rich fraction (TQRF) extracted from N. sativa seeds using supercritical fluid extraction (SFE) system and commercially available thymoquinone (TQ) on colon cancer (HT29), lymphoblastic leukemia (CEMSS) and promyelocytic leukemia (HL60) cells lines were investigated. The concentration that gave 50% inhibition of cell viability (IC50) of HT29, CEMSS and HL60 cells treated with TQRF were 400, 350 and 250 µg/ml, respectively. Meanwhile, the IC50 of TQ was 8, 5 and 3 µg/ml, respectively. Cell cycle analysis shows the increment of apoptosis in a time-dependent manner. However, both TQRF and TQ were not able to arrest the cell cycle phases of the cells. Apoptosis was the main mode of HT29, CEMSS and HL60 cells death induced by both TQRF and TQ. Our findings support the potential use of TQRF and TQ for the treatment of colon cancer and leukemia.
format Article
author Ismail, Norsharina
Ismail, Maznah
Al-Absi, Aied
Al-Naqeeb, Ghanya
spellingShingle Ismail, Norsharina
Ismail, Maznah
Al-Absi, Aied
Al-Naqeeb, Ghanya
Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines.
author_facet Ismail, Norsharina
Ismail, Maznah
Al-Absi, Aied
Al-Naqeeb, Ghanya
author_sort Ismail, Norsharina
title Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines.
title_short Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines.
title_full Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines.
title_fullStr Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines.
title_full_unstemmed Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines.
title_sort thymoquinone rich fraction from nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines.
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24493/1/Thymoquinone%20rich%20fraction%20from%20Nigella%20sativa%20and%20thymoquinone%20are%20cytotoxic%20towards%20colon%20and%20leukemic%20carcinoma%20cell%20lines.pdf
http://psasir.upm.edu.my/id/eprint/24493/
http://www.academicjournals.org/journal/JMPR
_version_ 1643828376904925184
score 13.211869

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