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Pneumococcal replicative state in relation to its adherence capacity to A549-cell line: a preliminary in vitro analysis

This study was to compare the replication capacity of pneumococcal isolates (serotypes 1, 7F, 19F and 23F) with their adherence pattern to monolayer cells (A549). For standardization purposes, all isolates showed a normal growth curve in both bacteriological (THB + 0.5% yeast extract with and withou...

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Main Authors: Mohd Desa, Mohd Nasir, Parasakthi, Navaratnam, Sekaran, Shamala Devi, Vadivelu, Jamunarani
Format: Article
Language:English
Published: Malaysian Society for Microbiology 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24491/1/Pneumococcal%20replicative%20state%20in%20relation%20to%20its%20adherence%20capacity%20to%20A549%20cell%20line.pdf
http://psasir.upm.edu.my/id/eprint/24491/
http://mjm.usm.my/index.php?r=cms/entry/view&id=51
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spelling my.upm.eprints.244912018-05-16T01:48:31Z http://psasir.upm.edu.my/id/eprint/24491/ Pneumococcal replicative state in relation to its adherence capacity to A549-cell line: a preliminary in vitro analysis Mohd Desa, Mohd Nasir Parasakthi, Navaratnam Sekaran, Shamala Devi Vadivelu, Jamunarani This study was to compare the replication capacity of pneumococcal isolates (serotypes 1, 7F, 19F and 23F) with their adherence pattern to monolayer cells (A549). For standardization purposes, all isolates showed a normal growth curve in both bacteriological (THB + 0.5% yeast extract with and without 2% FBS) and cell culture media (RPMI + 2% FBS). In the former media, a shorter lag phase was observed for isolate serotypes 1 and 7F in presence of serum while in the later; growth yield was lower for all isolates with stationary phase approaching OD600 of 0.01 as compared to 1.0 in bacteriological media. In the replicative analysis at different growth phases of the isolates in cell culture media, growth capacity at 3 h post-incubation was frequently twice as that at 1 h, and that at early-log phase was frequently higher than that at mid-log phase at both post-incubation times. Adherence was frequently the least at early-log phase although the isolates were in the most active state of replication to increase the number of pneumococcal cells to adhere. At mid- and late-log phases, pneumococcal adherence was frequently higher although the replication was reduced. This study marks the potential correlation between pneumococcal growth fitness and adherence capacity whereby the later may not be superior during the early growth phase. Malaysian Society for Microbiology 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24491/1/Pneumococcal%20replicative%20state%20in%20relation%20to%20its%20adherence%20capacity%20to%20A549%20cell%20line.pdf Mohd Desa, Mohd Nasir and Parasakthi, Navaratnam and Sekaran, Shamala Devi and Vadivelu, Jamunarani (2011) Pneumococcal replicative state in relation to its adherence capacity to A549-cell line: a preliminary in vitro analysis. Malaysian Journal of Microbiology, 7 (2). pp. 107-110. ISSN 1823-8262; ESSN: 2231-7538 http://mjm.usm.my/index.php?r=cms/entry/view&id=51 10.21161/mjm.26010
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study was to compare the replication capacity of pneumococcal isolates (serotypes 1, 7F, 19F and 23F) with their adherence pattern to monolayer cells (A549). For standardization purposes, all isolates showed a normal growth curve in both bacteriological (THB + 0.5% yeast extract with and without 2% FBS) and cell culture media (RPMI + 2% FBS). In the former media, a shorter lag phase was observed for isolate serotypes 1 and 7F in presence of serum while in the later; growth yield was lower for all isolates with stationary phase approaching OD600 of 0.01 as compared to 1.0 in bacteriological media. In the replicative analysis at different growth phases of the isolates in cell culture media, growth capacity at 3 h post-incubation was frequently twice as that at 1 h, and that at early-log phase was frequently higher than that at mid-log phase at both post-incubation times. Adherence was frequently the least at early-log phase although the isolates were in the most active state of replication to increase the number of pneumococcal cells to adhere. At mid- and late-log phases, pneumococcal adherence was frequently higher although the replication was reduced. This study marks the potential correlation between pneumococcal growth fitness and adherence capacity whereby the later may not be superior during the early growth phase.
format Article
author Mohd Desa, Mohd Nasir
Parasakthi, Navaratnam
Sekaran, Shamala Devi
Vadivelu, Jamunarani
spellingShingle Mohd Desa, Mohd Nasir
Parasakthi, Navaratnam
Sekaran, Shamala Devi
Vadivelu, Jamunarani
Pneumococcal replicative state in relation to its adherence capacity to A549-cell line: a preliminary in vitro analysis
author_facet Mohd Desa, Mohd Nasir
Parasakthi, Navaratnam
Sekaran, Shamala Devi
Vadivelu, Jamunarani
author_sort Mohd Desa, Mohd Nasir
title Pneumococcal replicative state in relation to its adherence capacity to A549-cell line: a preliminary in vitro analysis
title_short Pneumococcal replicative state in relation to its adherence capacity to A549-cell line: a preliminary in vitro analysis
title_full Pneumococcal replicative state in relation to its adherence capacity to A549-cell line: a preliminary in vitro analysis
title_fullStr Pneumococcal replicative state in relation to its adherence capacity to A549-cell line: a preliminary in vitro analysis
title_full_unstemmed Pneumococcal replicative state in relation to its adherence capacity to A549-cell line: a preliminary in vitro analysis
title_sort pneumococcal replicative state in relation to its adherence capacity to a549-cell line: a preliminary in vitro analysis
publisher Malaysian Society for Microbiology
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24491/1/Pneumococcal%20replicative%20state%20in%20relation%20to%20its%20adherence%20capacity%20to%20A549%20cell%20line.pdf
http://psasir.upm.edu.my/id/eprint/24491/
http://mjm.usm.my/index.php?r=cms/entry/view&id=51
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score 13.211869

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