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Pre-assessment of microbiology quality and antibiotic resistance of vibrio parahaemolyticus from cockle (Anadaragranosa) in Malaysia.

Introduction Vibrio species has shown one of the most important bacterial diseases in marine cultured organisms, affecting a large number of species of fish and shellfish. Among well known species is Vibrio parahaemolyticus which associated with cockle and reported as one of the important foodbor...

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Main Authors: Ariffin, Ahmad Azuhairi, A. M., Sahilah, R., Rahimah, A. S., Norrakiah, A. G., Maaruf, Abdul Rahman, Anita, Ibrahim, Faisal
Format: Article
Language:English
English
Published: Malaysian Public Health Specialist Association 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24486/2/Pre-assessment.pdf
http://psasir.upm.edu.my/id/eprint/24486/
http://www.mjphm.org.my/mjphm/
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spelling my.upm.eprints.244862015-10-20T08:49:34Z http://psasir.upm.edu.my/id/eprint/24486/ Pre-assessment of microbiology quality and antibiotic resistance of vibrio parahaemolyticus from cockle (Anadaragranosa) in Malaysia. Ariffin, Ahmad Azuhairi A. M., Sahilah R., Rahimah A. S., Norrakiah A. G., Maaruf Abdul Rahman, Anita Ibrahim, Faisal Introduction Vibrio species has shown one of the most important bacterial diseases in marine cultured organisms, affecting a large number of species of fish and shellfish. Among well known species is Vibrio parahaemolyticus which associated with cockle and reported as one of the important foodborne diseases in developing countries. Objectives The study was conducted to determine the microbiology quality of Vibrio parahaemolyticus in cockles (Anadaragranosa) in Selangor, Perak, Melaka and Negeri Sembilan. Methods A total of 12 cockle samples were purchased from wet market throughout 3 weeks in September 2009. Out of the 12 cockle samples, 120 isolates were randomly produced and tested for microbiology quality of V. parahaemolyticus by growing onto Thiosulphate Bile-Salt Sucrose (TCBS) agar. Tentative colonies of V. parahaemolyticus were then examined for biochemical test and antibiotic resistance patterns towards bacitracin, vancomycin, penicillin, chloramphenicol, amikacin, enrofloxacin and cifrofloxacin. Results The highest mean colony counting value for Vibrio grown onto TCBS agar was from Melaka with 4.19 x 105cfu per gm, followed by Perak (4.15 x 105cfu per gm), Negeri Sembilan (3.72 x 105cfu per gm) and Selangor with 1.58 x 105 cfu per gm. Biochemical tests showed 75 isolates were positive for V. parahaemolyticus with 29.3% (22/30) isolates were from Perak, 26.3% (20/30) isolates were from Selangor, 22.6% (17/30) isolates were from Melaka and 21.2% (16/30) isolates were from Negeri Sembilan. As for antibiotic resistance pattern, all V. parahaemolyticus isolates were resistant toward one or more antibiotic tested with 100% (75/75) isolates resistant toward bacitracin, 99% (74/75) toward vancomycinand 92% (69/75) toward penicillin. Inspite of this, none of the V. parahaemolyticus isolates were resistant toward chloramphenicol, amikacin, enrofloxacin and cifrofloxacin. Conclusion The presence of V. parahaemolyticus in all cockle samples with their antibiotic resistance properties was alarming. More samples should be studied in obtaining an accurate view of microbiology quality and antibiotic resistance of V. parahaemolyticusin cockle samples in Malaysia. Malaysian Public Health Specialist Association 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24486/2/Pre-assessment.pdf Ariffin, Ahmad Azuhairi and A. M., Sahilah and R., Rahimah and A. S., Norrakiah and A. G., Maaruf and Abdul Rahman, Anita and Ibrahim, Faisal (2011) Pre-assessment of microbiology quality and antibiotic resistance of vibrio parahaemolyticus from cockle (Anadaragranosa) in Malaysia. Malaysian Journal of Public Health Medicine, 11 (Suppl. 3). ISSN 1675-0306 http://www.mjphm.org.my/mjphm/ English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Introduction Vibrio species has shown one of the most important bacterial diseases in marine cultured organisms, affecting a large number of species of fish and shellfish. Among well known species is Vibrio parahaemolyticus which associated with cockle and reported as one of the important foodborne diseases in developing countries. Objectives The study was conducted to determine the microbiology quality of Vibrio parahaemolyticus in cockles (Anadaragranosa) in Selangor, Perak, Melaka and Negeri Sembilan. Methods A total of 12 cockle samples were purchased from wet market throughout 3 weeks in September 2009. Out of the 12 cockle samples, 120 isolates were randomly produced and tested for microbiology quality of V. parahaemolyticus by growing onto Thiosulphate Bile-Salt Sucrose (TCBS) agar. Tentative colonies of V. parahaemolyticus were then examined for biochemical test and antibiotic resistance patterns towards bacitracin, vancomycin, penicillin, chloramphenicol, amikacin, enrofloxacin and cifrofloxacin. Results The highest mean colony counting value for Vibrio grown onto TCBS agar was from Melaka with 4.19 x 105cfu per gm, followed by Perak (4.15 x 105cfu per gm), Negeri Sembilan (3.72 x 105cfu per gm) and Selangor with 1.58 x 105 cfu per gm. Biochemical tests showed 75 isolates were positive for V. parahaemolyticus with 29.3% (22/30) isolates were from Perak, 26.3% (20/30) isolates were from Selangor, 22.6% (17/30) isolates were from Melaka and 21.2% (16/30) isolates were from Negeri Sembilan. As for antibiotic resistance pattern, all V. parahaemolyticus isolates were resistant toward one or more antibiotic tested with 100% (75/75) isolates resistant toward bacitracin, 99% (74/75) toward vancomycinand 92% (69/75) toward penicillin. Inspite of this, none of the V. parahaemolyticus isolates were resistant toward chloramphenicol, amikacin, enrofloxacin and cifrofloxacin. Conclusion The presence of V. parahaemolyticus in all cockle samples with their antibiotic resistance properties was alarming. More samples should be studied in obtaining an accurate view of microbiology quality and antibiotic resistance of V. parahaemolyticusin cockle samples in Malaysia.
format Article
author Ariffin, Ahmad Azuhairi
A. M., Sahilah
R., Rahimah
A. S., Norrakiah
A. G., Maaruf
Abdul Rahman, Anita
Ibrahim, Faisal
spellingShingle Ariffin, Ahmad Azuhairi
A. M., Sahilah
R., Rahimah
A. S., Norrakiah
A. G., Maaruf
Abdul Rahman, Anita
Ibrahim, Faisal
Pre-assessment of microbiology quality and antibiotic resistance of vibrio parahaemolyticus from cockle (Anadaragranosa) in Malaysia.
author_facet Ariffin, Ahmad Azuhairi
A. M., Sahilah
R., Rahimah
A. S., Norrakiah
A. G., Maaruf
Abdul Rahman, Anita
Ibrahim, Faisal
author_sort Ariffin, Ahmad Azuhairi
title Pre-assessment of microbiology quality and antibiotic resistance of vibrio parahaemolyticus from cockle (Anadaragranosa) in Malaysia.
title_short Pre-assessment of microbiology quality and antibiotic resistance of vibrio parahaemolyticus from cockle (Anadaragranosa) in Malaysia.
title_full Pre-assessment of microbiology quality and antibiotic resistance of vibrio parahaemolyticus from cockle (Anadaragranosa) in Malaysia.
title_fullStr Pre-assessment of microbiology quality and antibiotic resistance of vibrio parahaemolyticus from cockle (Anadaragranosa) in Malaysia.
title_full_unstemmed Pre-assessment of microbiology quality and antibiotic resistance of vibrio parahaemolyticus from cockle (Anadaragranosa) in Malaysia.
title_sort pre-assessment of microbiology quality and antibiotic resistance of vibrio parahaemolyticus from cockle (anadaragranosa) in malaysia.
publisher Malaysian Public Health Specialist Association
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24486/2/Pre-assessment.pdf
http://psasir.upm.edu.my/id/eprint/24486/
http://www.mjphm.org.my/mjphm/
_version_ 1643828374709207040
score 13.211869

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