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Analysis of three polymorphisms in Bidayuh ethnic of Sarawak population: a report from Malaysia

Insertion/deletion polymorphism of YAP (DYS287), M96 and M120 polymorphisms in Bidayuh ethnic populations of Sarawak, Malaysia were analyzed in this study. Genomic DNA was extracted from 180 buccal samples and amplified by Hot-Start PCR method. The amplified PCR products were separated by using 2% a...

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Main Authors: Vasudevan, Ramachandran, C. P. N., Fathihah, Ismail, Patimah
Format: Article
Language:English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24400/1/24400.pdf
http://psasir.upm.edu.my/id/eprint/24400/
http://www.academicjournals.org/journal/AJB/article-abstract/77B351F34743
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spelling my.upm.eprints.244002016-04-27T04:22:28Z http://psasir.upm.edu.my/id/eprint/24400/ Analysis of three polymorphisms in Bidayuh ethnic of Sarawak population: a report from Malaysia Vasudevan, Ramachandran C. P. N., Fathihah Ismail, Patimah Insertion/deletion polymorphism of YAP (DYS287), M96 and M120 polymorphisms in Bidayuh ethnic populations of Sarawak, Malaysia were analyzed in this study. Genomic DNA was extracted from 180 buccal samples and amplified by Hot-Start PCR method. The amplified PCR products were separated by using 2% agarose gel electrophoresis. 26 out of 180 samples (14.44%) have shown insertion (YAP+) polymorphism, while the rest of the samples (85.56%) have shown deletion (YAP-) polymorphism. M96 derived and ancestral allele reveal 102 (56.67%) and 78 (43.33%), respectively and 96 (53.33%), while for M120 derived allele and 84 (46.67%) for M120 ancestral allele. This study has provided the information about the distribution of YAP, M96 and M120 polymorphisms in Bidayuh ethnic population of Sarawak. This study has to be continued with more samples to determine the YAP polymorphism and also the biallelic markers in Bidayuh population. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24400/1/24400.pdf Vasudevan, Ramachandran and C. P. N., Fathihah and Ismail, Patimah (2011) Analysis of three polymorphisms in Bidayuh ethnic of Sarawak population: a report from Malaysia. African Journal of Biotechnology, 10 (22). art. no. 77B351F34743. pp. 4544-4549. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/77B351F34743
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Insertion/deletion polymorphism of YAP (DYS287), M96 and M120 polymorphisms in Bidayuh ethnic populations of Sarawak, Malaysia were analyzed in this study. Genomic DNA was extracted from 180 buccal samples and amplified by Hot-Start PCR method. The amplified PCR products were separated by using 2% agarose gel electrophoresis. 26 out of 180 samples (14.44%) have shown insertion (YAP+) polymorphism, while the rest of the samples (85.56%) have shown deletion (YAP-) polymorphism. M96 derived and ancestral allele reveal 102 (56.67%) and 78 (43.33%), respectively and 96 (53.33%), while for M120 derived allele and 84 (46.67%) for M120 ancestral allele. This study has provided the information about the distribution of YAP, M96 and M120 polymorphisms in Bidayuh ethnic population of Sarawak. This study has to be continued with more samples to determine the YAP polymorphism and also the biallelic markers in Bidayuh population.
format Article
author Vasudevan, Ramachandran
C. P. N., Fathihah
Ismail, Patimah
spellingShingle Vasudevan, Ramachandran
C. P. N., Fathihah
Ismail, Patimah
Analysis of three polymorphisms in Bidayuh ethnic of Sarawak population: a report from Malaysia
author_facet Vasudevan, Ramachandran
C. P. N., Fathihah
Ismail, Patimah
author_sort Vasudevan, Ramachandran
title Analysis of three polymorphisms in Bidayuh ethnic of Sarawak population: a report from Malaysia
title_short Analysis of three polymorphisms in Bidayuh ethnic of Sarawak population: a report from Malaysia
title_full Analysis of three polymorphisms in Bidayuh ethnic of Sarawak population: a report from Malaysia
title_fullStr Analysis of three polymorphisms in Bidayuh ethnic of Sarawak population: a report from Malaysia
title_full_unstemmed Analysis of three polymorphisms in Bidayuh ethnic of Sarawak population: a report from Malaysia
title_sort analysis of three polymorphisms in bidayuh ethnic of sarawak population: a report from malaysia
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24400/1/24400.pdf
http://psasir.upm.edu.my/id/eprint/24400/
http://www.academicjournals.org/journal/AJB/article-abstract/77B351F34743
_version_ 1643828349516120064
score 13.211869

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