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A quick DNA extraction protocol: without liquid nitrogen in ambient temperature

Marker assisted selection is an effective technique for quality traits selection in breeding program which are impossible by visual observation. Marker assisted selection in early generation requires rapid DNA extraction protocol for large number of samples in a low cost approach. A rapid and inexpe...

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Main Authors: Ferdous, Jannatul, Musa, Mohamed Hanafi, Yusop, Mohd Rafii, Syed Muhammad, Sharifah Kharidah
Format: Article
Language:English
Published: Academic Journals 2012
Online Access:http://psasir.upm.edu.my/id/eprint/24185/1/24185.pdf
http://psasir.upm.edu.my/id/eprint/24185/
http://academicjournals.org/journal/AJB/article-abstract/197554430636
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spelling my.upm.eprints.241852017-11-09T09:25:26Z http://psasir.upm.edu.my/id/eprint/24185/ A quick DNA extraction protocol: without liquid nitrogen in ambient temperature Ferdous, Jannatul Musa, Mohamed Hanafi Yusop, Mohd Rafii Syed Muhammad, Sharifah Kharidah Marker assisted selection is an effective technique for quality traits selection in breeding program which are impossible by visual observation. Marker assisted selection in early generation requires rapid DNA extraction protocol for large number of samples in a low cost approach. A rapid and inexpensive DNA extraction protocol has been described for different tissues of color rice and other plant species which contain pigment and polyphenolic compound. This method has been modified from well known cetyltrimethylammonium bromide (CTAB) method where CTAB is used for DNA extraction. This protocol is simple and fast compared to other methods and no liquid nitrogen is required. Only inexpensive chemicals and ordinary laboratory equipments are enough for DNA extraction. The quantity of total genomic DNA from different tissues was almost similar which was extracted from 10 mg samples. The extracted DNA is stable and applicable to marker assisted selection, DNA fingerprinting, quantitative traits loci analysis, screening of transformants and enzymatic digestion. Academic Journals 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24185/1/24185.pdf Ferdous, Jannatul and Musa, Mohamed Hanafi and Yusop, Mohd Rafii and Syed Muhammad, Sharifah Kharidah (2012) A quick DNA extraction protocol: without liquid nitrogen in ambient temperature. African Journal of Biotechnology, 11 (27). art. no. 197554430636. pp. 6956-6964. ISSN 1684–5315 http://academicjournals.org/journal/AJB/article-abstract/197554430636 10.5897/AJB11.3283
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Marker assisted selection is an effective technique for quality traits selection in breeding program which are impossible by visual observation. Marker assisted selection in early generation requires rapid DNA extraction protocol for large number of samples in a low cost approach. A rapid and inexpensive DNA extraction protocol has been described for different tissues of color rice and other plant species which contain pigment and polyphenolic compound. This method has been modified from well known cetyltrimethylammonium bromide (CTAB) method where CTAB is used for DNA extraction. This protocol is simple and fast compared to other methods and no liquid nitrogen is required. Only inexpensive chemicals and ordinary laboratory equipments are enough for DNA extraction. The quantity of total genomic DNA from different tissues was almost similar which was extracted from 10 mg samples. The extracted DNA is stable and applicable to marker assisted selection, DNA fingerprinting, quantitative traits loci analysis, screening of transformants and enzymatic digestion.
format Article
author Ferdous, Jannatul
Musa, Mohamed Hanafi
Yusop, Mohd Rafii
Syed Muhammad, Sharifah Kharidah
spellingShingle Ferdous, Jannatul
Musa, Mohamed Hanafi
Yusop, Mohd Rafii
Syed Muhammad, Sharifah Kharidah
A quick DNA extraction protocol: without liquid nitrogen in ambient temperature
author_facet Ferdous, Jannatul
Musa, Mohamed Hanafi
Yusop, Mohd Rafii
Syed Muhammad, Sharifah Kharidah
author_sort Ferdous, Jannatul
title A quick DNA extraction protocol: without liquid nitrogen in ambient temperature
title_short A quick DNA extraction protocol: without liquid nitrogen in ambient temperature
title_full A quick DNA extraction protocol: without liquid nitrogen in ambient temperature
title_fullStr A quick DNA extraction protocol: without liquid nitrogen in ambient temperature
title_full_unstemmed A quick DNA extraction protocol: without liquid nitrogen in ambient temperature
title_sort quick dna extraction protocol: without liquid nitrogen in ambient temperature
publisher Academic Journals
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/24185/1/24185.pdf
http://psasir.upm.edu.my/id/eprint/24185/
http://academicjournals.org/journal/AJB/article-abstract/197554430636
_version_ 1643828285226876928
score 13.211869

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