Cover Image

Optimization of HPLC conditions for quantitative analysis of aflatoxins in contaminated peanut.

The main objective of present study was to investigate the effect of HPLC conditions namely mobile phase composition (X1), flow rate (X2) and temperature (X3) on peak area of four target aflatoxins (i.e. B1 (Y1), B2 (Y2), G1 (Y3) and G2 (Y4)) from the spiked peanut. The significant nonlinear respons...

Full description

Saved in:
Bibliographic Details
Main Authors: Afsah-Hejri, Leili, Selamat, Jinap, Arzandeh, Sahar, Mirhosseini, Hamed
Format: Article
Language:English
English
Published: Elsevier 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24130/1/Optimization%20of%20HPLC%20conditions%20for%20quantitative%20analysis%20of%20aflatoxins%20in%20contaminated%20peanut.pdf
http://psasir.upm.edu.my/id/eprint/24130/
http://www.elsevier.com/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.24130
record_format eprints
spelling my.upm.eprints.241302015-10-02T02:47:13Z http://psasir.upm.edu.my/id/eprint/24130/ Optimization of HPLC conditions for quantitative analysis of aflatoxins in contaminated peanut. Afsah-Hejri, Leili Selamat, Jinap Arzandeh, Sahar Mirhosseini, Hamed The main objective of present study was to investigate the effect of HPLC conditions namely mobile phase composition (X1), flow rate (X2) and temperature (X3) on peak area of four target aflatoxins (i.e. B1 (Y1), B2 (Y2), G1 (Y3) and G2 (Y4)) from the spiked peanut. The significant nonlinear response surface models with high coefficient of determinations (R2) ranging from 0.958 to 0.995 were fitted to evaluate the detection value of target aflatoxins as a function of HPLC variables. Flow rate had the most significant (p < 0.05) effect on quantification value of target aflatoxins. The highest quantification value for target aflatoxins could be obtained under the following HPLC conditions: the mobile phase composition of ACN/H2O/MeOH: 8/54/38, temperature of 24 °C and flow rate of 0.4 mL/min. The recommended optimum HPLC conditions provided higher peak area for all target aflatoxins by 1-2.5 fold compared to two other conditions (A: mobile phase (ACN/H2O/MeOH: 23/54/23), ambient temperature (28-32 °C), flow rate 1 mL/min; B: mobile phase (ACN/H2O/MeOH: 17/54/29), temperature 30 °C, flow rate 1 mL/min). Elsevier 2011-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24130/1/Optimization%20of%20HPLC%20conditions%20for%20quantitative%20analysis%20of%20aflatoxins%20in%20contaminated%20peanut.pdf Afsah-Hejri, Leili and Selamat, Jinap and Arzandeh, Sahar and Mirhosseini, Hamed (2011) Optimization of HPLC conditions for quantitative analysis of aflatoxins in contaminated peanut. Food Control, 22 (3-4 ). pp. 381-388. ISSN 0956-7135 http://www.elsevier.com/ 10.1016/j.foodcont.2010.09.007 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The main objective of present study was to investigate the effect of HPLC conditions namely mobile phase composition (X1), flow rate (X2) and temperature (X3) on peak area of four target aflatoxins (i.e. B1 (Y1), B2 (Y2), G1 (Y3) and G2 (Y4)) from the spiked peanut. The significant nonlinear response surface models with high coefficient of determinations (R2) ranging from 0.958 to 0.995 were fitted to evaluate the detection value of target aflatoxins as a function of HPLC variables. Flow rate had the most significant (p < 0.05) effect on quantification value of target aflatoxins. The highest quantification value for target aflatoxins could be obtained under the following HPLC conditions: the mobile phase composition of ACN/H2O/MeOH: 8/54/38, temperature of 24 °C and flow rate of 0.4 mL/min. The recommended optimum HPLC conditions provided higher peak area for all target aflatoxins by 1-2.5 fold compared to two other conditions (A: mobile phase (ACN/H2O/MeOH: 23/54/23), ambient temperature (28-32 °C), flow rate 1 mL/min; B: mobile phase (ACN/H2O/MeOH: 17/54/29), temperature 30 °C, flow rate 1 mL/min).
format Article
author Afsah-Hejri, Leili
Selamat, Jinap
Arzandeh, Sahar
Mirhosseini, Hamed
spellingShingle Afsah-Hejri, Leili
Selamat, Jinap
Arzandeh, Sahar
Mirhosseini, Hamed
Optimization of HPLC conditions for quantitative analysis of aflatoxins in contaminated peanut.
author_facet Afsah-Hejri, Leili
Selamat, Jinap
Arzandeh, Sahar
Mirhosseini, Hamed
author_sort Afsah-Hejri, Leili
title Optimization of HPLC conditions for quantitative analysis of aflatoxins in contaminated peanut.
title_short Optimization of HPLC conditions for quantitative analysis of aflatoxins in contaminated peanut.
title_full Optimization of HPLC conditions for quantitative analysis of aflatoxins in contaminated peanut.
title_fullStr Optimization of HPLC conditions for quantitative analysis of aflatoxins in contaminated peanut.
title_full_unstemmed Optimization of HPLC conditions for quantitative analysis of aflatoxins in contaminated peanut.
title_sort optimization of hplc conditions for quantitative analysis of aflatoxins in contaminated peanut.
publisher Elsevier
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24130/1/Optimization%20of%20HPLC%20conditions%20for%20quantitative%20analysis%20of%20aflatoxins%20in%20contaminated%20peanut.pdf
http://psasir.upm.edu.my/id/eprint/24130/
http://www.elsevier.com/
_version_ 1643828268619530240
score 13.211869

Similar Items