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Enhancement of thermostable lipase production by a genotypically identified extremophilic Bacillus subtilis NS 8 in a continuous bioreactor.

Bacillus strain NS 8, a lipase-producing bacterium isolated from a Malaysian hot spring, is able to tolerate a broad range of temperature and pH, which makes it beneficial for this study. It generated PCR products with molecular weight of 1,532 bp, and the 16S rRNA sequence analysis identified it as...

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Main Authors: Olusesan, Akanbi Taiwo, L. Kamaruzaman, Azura, Abu Bakar, Fatimah, Mohammed, Abdulkarim Sabo, Radu, Son, Abd. Manap, Mohd. Yazid, Saari, Nazamid
Format: Article
Language:English
Published: Karger 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24072/
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spelling my.upm.eprints.240722014-02-18T09:26:01Z http://psasir.upm.edu.my/id/eprint/24072/ Enhancement of thermostable lipase production by a genotypically identified extremophilic Bacillus subtilis NS 8 in a continuous bioreactor. Olusesan, Akanbi Taiwo L. Kamaruzaman, Azura Abu Bakar, Fatimah Mohammed, Abdulkarim Sabo Radu, Son Abd. Manap, Mohd. Yazid Saari, Nazamid Bacillus strain NS 8, a lipase-producing bacterium isolated from a Malaysian hot spring, is able to tolerate a broad range of temperature and pH, which makes it beneficial for this study. It generated PCR products with molecular weight of 1,532 bp, and the 16S rRNA sequence analysis identified it as Bacillus subtilis with accession number AB110598. It showed a 71% similarity index with B. subtilis using Biolog Microstation System. Its lipase production was optimized using a shake flask system by changing the physical (agitation speed, pH and temperature) and nutritional (nitrogen, carbon and minerals) factors. The most suitable combination of the basal medium for lipase production was 2.5% olive oil (carbon), 1.5% peptone (nitrogen), 0.1% MgSO 4 (mineral) at an optimum temperature of 50°C, pH 7.5 and 150 rpm agitation, giving an enzyme yield of 4.23 U/ml. Statistical optimization using response surface methodology was carried out. An optimum lipase production of 5.67 U/ml was achieved when olive oil concentration of 3%, peptone 2%, MgSO 4·7H 2O 0.2% and an agitation rate of 200 rpm were combined. Lipase production was further carried out inside a 2-liter bioreactor, which yielded an enzyme activity of 14.5 U/ml after 15 h of incubation. Karger 2011 Article PeerReviewed Olusesan, Akanbi Taiwo and L. Kamaruzaman, Azura and Abu Bakar, Fatimah and Mohammed, Abdulkarim Sabo and Radu, Son and Abd. Manap, Mohd. Yazid and Saari, Nazamid (2011) Enhancement of thermostable lipase production by a genotypically identified extremophilic Bacillus subtilis NS 8 in a continuous bioreactor. Journal of Molecular Microbiology and Biotechnology, 20 (2 ). pp. 105-115. ISSN 1464-1801 10.1159/000324535 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Bacillus strain NS 8, a lipase-producing bacterium isolated from a Malaysian hot spring, is able to tolerate a broad range of temperature and pH, which makes it beneficial for this study. It generated PCR products with molecular weight of 1,532 bp, and the 16S rRNA sequence analysis identified it as Bacillus subtilis with accession number AB110598. It showed a 71% similarity index with B. subtilis using Biolog Microstation System. Its lipase production was optimized using a shake flask system by changing the physical (agitation speed, pH and temperature) and nutritional (nitrogen, carbon and minerals) factors. The most suitable combination of the basal medium for lipase production was 2.5% olive oil (carbon), 1.5% peptone (nitrogen), 0.1% MgSO 4 (mineral) at an optimum temperature of 50°C, pH 7.5 and 150 rpm agitation, giving an enzyme yield of 4.23 U/ml. Statistical optimization using response surface methodology was carried out. An optimum lipase production of 5.67 U/ml was achieved when olive oil concentration of 3%, peptone 2%, MgSO 4·7H 2O 0.2% and an agitation rate of 200 rpm were combined. Lipase production was further carried out inside a 2-liter bioreactor, which yielded an enzyme activity of 14.5 U/ml after 15 h of incubation.
format Article
author Olusesan, Akanbi Taiwo
L. Kamaruzaman, Azura
Abu Bakar, Fatimah
Mohammed, Abdulkarim Sabo
Radu, Son
Abd. Manap, Mohd. Yazid
Saari, Nazamid
spellingShingle Olusesan, Akanbi Taiwo
L. Kamaruzaman, Azura
Abu Bakar, Fatimah
Mohammed, Abdulkarim Sabo
Radu, Son
Abd. Manap, Mohd. Yazid
Saari, Nazamid
Enhancement of thermostable lipase production by a genotypically identified extremophilic Bacillus subtilis NS 8 in a continuous bioreactor.
author_facet Olusesan, Akanbi Taiwo
L. Kamaruzaman, Azura
Abu Bakar, Fatimah
Mohammed, Abdulkarim Sabo
Radu, Son
Abd. Manap, Mohd. Yazid
Saari, Nazamid
author_sort Olusesan, Akanbi Taiwo
title Enhancement of thermostable lipase production by a genotypically identified extremophilic Bacillus subtilis NS 8 in a continuous bioreactor.
title_short Enhancement of thermostable lipase production by a genotypically identified extremophilic Bacillus subtilis NS 8 in a continuous bioreactor.
title_full Enhancement of thermostable lipase production by a genotypically identified extremophilic Bacillus subtilis NS 8 in a continuous bioreactor.
title_fullStr Enhancement of thermostable lipase production by a genotypically identified extremophilic Bacillus subtilis NS 8 in a continuous bioreactor.
title_full_unstemmed Enhancement of thermostable lipase production by a genotypically identified extremophilic Bacillus subtilis NS 8 in a continuous bioreactor.
title_sort enhancement of thermostable lipase production by a genotypically identified extremophilic bacillus subtilis ns 8 in a continuous bioreactor.
publisher Karger
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24072/
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score 13.211869

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