Cover Image

Rapid detection and E-test antimicrobial susceptibility testing of Vibrio parahaemolyticus isolated from seafood and environmental sources in Malaysia.

Objectives: To find out the prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus in seafoods and environmental sources. Methods: The study was carried out at the Center of Excellence for Food Safety Research, University Putra Malaysia; Universiti Kebangsaan Malaysia; Medical Mo...

Full description

Saved in:
Bibliographic Details
Main Authors: Al-Othrubi, Saleh M., Hanafiah, Alfizah, Radu, Son, Neoh, Humin, Jamal, Rahaman
Format: Article
Language:English
English
Published: Saudi Arabian Armed Forces Hospital 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24058/1/Rapid%20detection%20and%20E.pdf
http://psasir.upm.edu.my/id/eprint/24058/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.24058
record_format eprints
spelling my.upm.eprints.240582015-10-21T02:42:52Z http://psasir.upm.edu.my/id/eprint/24058/ Rapid detection and E-test antimicrobial susceptibility testing of Vibrio parahaemolyticus isolated from seafood and environmental sources in Malaysia. Al-Othrubi, Saleh M. Hanafiah, Alfizah Radu, Son Neoh, Humin Jamal, Rahaman Objectives: To find out the prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus in seafoods and environmental sources. Methods: The study was carried out at the Center of Excellence for Food Safety Research, University Putra Malaysia; Universiti Kebangsaan Malaysia; Medical Molecular Biology Institute; and University Kebansaan Malaysia Hospital, Malaysia between January 2006 and August 2008. One hundred and forty-four isolates from 400 samples of seafood (122 isolates) and seawater sources (22 isolates) were investigated for the presence of thermostable direct hemolysin (tdh+) and TDH-related hemolysin (trh+) genes using the standard methods. The E-test method was used to test the antimicrobial susceptibility. Results: The study indicates low occurrence of tdh+ (0.69%) and trh+ isolates (8.3%). None of the isolates tested posses both virulence genes. High sensitivity was observed against tetracycline (98%). The mean minimum inhibitory concentration (MIC) of the isolates toward ampicillin increased from 4 ug/ml in 2004 to 24 ug/ml in 2007. Conclusions: The current study demonstrates a low occurrence of pathogenic Vibrio parahaemolyticus in the marine environment and seafood. Nonetheless, the potential risk of vibrio infection due to consumption of Vibrio parahaemolyticus contaminated seafood in Malaysia should not be neglected. Saudi Arabian Armed Forces Hospital 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24058/1/Rapid%20detection%20and%20E.pdf Al-Othrubi, Saleh M. and Hanafiah, Alfizah and Radu, Son and Neoh, Humin and Jamal, Rahaman (2011) Rapid detection and E-test antimicrobial susceptibility testing of Vibrio parahaemolyticus isolated from seafood and environmental sources in Malaysia. Saudi Medical Journal, 32 (4). pp. 400-406. ISSN 0379-5284; ESSN: 1658-3175 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Objectives: To find out the prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus in seafoods and environmental sources. Methods: The study was carried out at the Center of Excellence for Food Safety Research, University Putra Malaysia; Universiti Kebangsaan Malaysia; Medical Molecular Biology Institute; and University Kebansaan Malaysia Hospital, Malaysia between January 2006 and August 2008. One hundred and forty-four isolates from 400 samples of seafood (122 isolates) and seawater sources (22 isolates) were investigated for the presence of thermostable direct hemolysin (tdh+) and TDH-related hemolysin (trh+) genes using the standard methods. The E-test method was used to test the antimicrobial susceptibility. Results: The study indicates low occurrence of tdh+ (0.69%) and trh+ isolates (8.3%). None of the isolates tested posses both virulence genes. High sensitivity was observed against tetracycline (98%). The mean minimum inhibitory concentration (MIC) of the isolates toward ampicillin increased from 4 ug/ml in 2004 to 24 ug/ml in 2007. Conclusions: The current study demonstrates a low occurrence of pathogenic Vibrio parahaemolyticus in the marine environment and seafood. Nonetheless, the potential risk of vibrio infection due to consumption of Vibrio parahaemolyticus contaminated seafood in Malaysia should not be neglected.
format Article
author Al-Othrubi, Saleh M.
Hanafiah, Alfizah
Radu, Son
Neoh, Humin
Jamal, Rahaman
spellingShingle Al-Othrubi, Saleh M.
Hanafiah, Alfizah
Radu, Son
Neoh, Humin
Jamal, Rahaman
Rapid detection and E-test antimicrobial susceptibility testing of Vibrio parahaemolyticus isolated from seafood and environmental sources in Malaysia.
author_facet Al-Othrubi, Saleh M.
Hanafiah, Alfizah
Radu, Son
Neoh, Humin
Jamal, Rahaman
author_sort Al-Othrubi, Saleh M.
title Rapid detection and E-test antimicrobial susceptibility testing of Vibrio parahaemolyticus isolated from seafood and environmental sources in Malaysia.
title_short Rapid detection and E-test antimicrobial susceptibility testing of Vibrio parahaemolyticus isolated from seafood and environmental sources in Malaysia.
title_full Rapid detection and E-test antimicrobial susceptibility testing of Vibrio parahaemolyticus isolated from seafood and environmental sources in Malaysia.
title_fullStr Rapid detection and E-test antimicrobial susceptibility testing of Vibrio parahaemolyticus isolated from seafood and environmental sources in Malaysia.
title_full_unstemmed Rapid detection and E-test antimicrobial susceptibility testing of Vibrio parahaemolyticus isolated from seafood and environmental sources in Malaysia.
title_sort rapid detection and e-test antimicrobial susceptibility testing of vibrio parahaemolyticus isolated from seafood and environmental sources in malaysia.
publisher Saudi Arabian Armed Forces Hospital
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/24058/1/Rapid%20detection%20and%20E.pdf
http://psasir.upm.edu.my/id/eprint/24058/
_version_ 1643828247028301824
score 13.211869

Similar Items