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Kenaf seed oil from supercritical carbon dioxide fluid extraction shows cytotoxic effects towards various cancer cell lines

Hibiscus cannabinus (Kenaf) from the family of Malvaceae is a valuable fiber source and medicinal plant. It has long been prescribed as traditional folk medicine in Africa and India to treat various diseases. Nevertheless, little research has been carried out on the potentials of this plant as treat...

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Main Authors: Saiful Yazan, Latifah, Foo, Jhi Biau, Chan, Kim Wei, Md. Tahir, Paridah, Ismail, Maznah
Format: Article
Language:English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/23892/1/23892.pdf
http://psasir.upm.edu.my/id/eprint/23892/
http://www.academicjournals.org/journal/AJB/article-abstract/5228AF534058
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spelling my.upm.eprints.238922016-05-31T07:46:25Z http://psasir.upm.edu.my/id/eprint/23892/ Kenaf seed oil from supercritical carbon dioxide fluid extraction shows cytotoxic effects towards various cancer cell lines Saiful Yazan, Latifah Foo, Jhi Biau Chan, Kim Wei Md. Tahir, Paridah Ismail, Maznah Hibiscus cannabinus (Kenaf) from the family of Malvaceae is a valuable fiber source and medicinal plant. It has long been prescribed as traditional folk medicine in Africa and India to treat various diseases. Nevertheless, little research has been carried out on the potentials of this plant as treatment for cancer. This study was designed to determine the cytotoxicity of kenaf seed oil from two varieties (Quiping 3 and V36) extracted by supercritical carbon dioxide fluid extraction (SFE) with different combinations of pressure (bars) and temperature (°C) towards breast cancer (MCF-7, MDA-MB-231, 4T1), cervical cancer (HeLa), lung cancer (A549) and leukemic (MOLT-4) cell lines. Even though kenaf seed oil from both varieties were cytotoxic to all the cancer cells, kenaf seed oil variety V36 extracted by SFE at 600 bars 40°C (V600/40) was the strongest towards MOLT-4 and MDA-MB-231, with the IC50 values of 153.26 and 483.35 μg/ml, respectively. MOLT-4 and MDA-MB-231 cells treated with V600/40 exhibited typical characteristics of apoptosis such as blebbing, chromatin condensation and nuclear fragmentation as viewed under an inverted light microscope and a fluorescence microscope. In conclusion, V600/40 was the most cytotoxic towards the MOLT-4 and MDA-MB-231 cells in a dosedependent manner possibly via the induction of apoptosis. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23892/1/23892.pdf Saiful Yazan, Latifah and Foo, Jhi Biau and Chan, Kim Wei and Md. Tahir, Paridah and Ismail, Maznah (2011) Kenaf seed oil from supercritical carbon dioxide fluid extraction shows cytotoxic effects towards various cancer cell lines. African Journal of Biotechnology, 10 (27). art. no. 5228AF534058. pp. 5381-5388. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/5228AF534058 10.5897/AJB10.2390
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Hibiscus cannabinus (Kenaf) from the family of Malvaceae is a valuable fiber source and medicinal plant. It has long been prescribed as traditional folk medicine in Africa and India to treat various diseases. Nevertheless, little research has been carried out on the potentials of this plant as treatment for cancer. This study was designed to determine the cytotoxicity of kenaf seed oil from two varieties (Quiping 3 and V36) extracted by supercritical carbon dioxide fluid extraction (SFE) with different combinations of pressure (bars) and temperature (°C) towards breast cancer (MCF-7, MDA-MB-231, 4T1), cervical cancer (HeLa), lung cancer (A549) and leukemic (MOLT-4) cell lines. Even though kenaf seed oil from both varieties were cytotoxic to all the cancer cells, kenaf seed oil variety V36 extracted by SFE at 600 bars 40°C (V600/40) was the strongest towards MOLT-4 and MDA-MB-231, with the IC50 values of 153.26 and 483.35 μg/ml, respectively. MOLT-4 and MDA-MB-231 cells treated with V600/40 exhibited typical characteristics of apoptosis such as blebbing, chromatin condensation and nuclear fragmentation as viewed under an inverted light microscope and a fluorescence microscope. In conclusion, V600/40 was the most cytotoxic towards the MOLT-4 and MDA-MB-231 cells in a dosedependent manner possibly via the induction of apoptosis.
format Article
author Saiful Yazan, Latifah
Foo, Jhi Biau
Chan, Kim Wei
Md. Tahir, Paridah
Ismail, Maznah
spellingShingle Saiful Yazan, Latifah
Foo, Jhi Biau
Chan, Kim Wei
Md. Tahir, Paridah
Ismail, Maznah
Kenaf seed oil from supercritical carbon dioxide fluid extraction shows cytotoxic effects towards various cancer cell lines
author_facet Saiful Yazan, Latifah
Foo, Jhi Biau
Chan, Kim Wei
Md. Tahir, Paridah
Ismail, Maznah
author_sort Saiful Yazan, Latifah
title Kenaf seed oil from supercritical carbon dioxide fluid extraction shows cytotoxic effects towards various cancer cell lines
title_short Kenaf seed oil from supercritical carbon dioxide fluid extraction shows cytotoxic effects towards various cancer cell lines
title_full Kenaf seed oil from supercritical carbon dioxide fluid extraction shows cytotoxic effects towards various cancer cell lines
title_fullStr Kenaf seed oil from supercritical carbon dioxide fluid extraction shows cytotoxic effects towards various cancer cell lines
title_full_unstemmed Kenaf seed oil from supercritical carbon dioxide fluid extraction shows cytotoxic effects towards various cancer cell lines
title_sort kenaf seed oil from supercritical carbon dioxide fluid extraction shows cytotoxic effects towards various cancer cell lines
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/23892/1/23892.pdf
http://psasir.upm.edu.my/id/eprint/23892/
http://www.academicjournals.org/journal/AJB/article-abstract/5228AF534058
_version_ 1643828197565923328
score 13.211869

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