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First report of Diaporthe/Phomopsis complex isolates in soybean from Malaysia and their longevity in stored seeds.

Diaporthe/Phomopsis Complex (DPC) can infect soybean seed and reduce its germination in the field and also survive on the seed during storage. Ten soybean seed lots which were stored at 0°C for up to 13 years were used to evaluate the percentage of DPC infection on them and also to identify the DPC...

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Main Authors: Raeisi, Samiyeh, Puteh, Adam, Sijam, Kamaruzaman
Format: Article
Language:English
English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/23794/2/First%20report%20of%20Diaporthe.pdf
http://psasir.upm.edu.my/id/eprint/23794/
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spelling my.upm.eprints.237942015-11-02T01:34:28Z http://psasir.upm.edu.my/id/eprint/23794/ First report of Diaporthe/Phomopsis complex isolates in soybean from Malaysia and their longevity in stored seeds. Raeisi, Samiyeh Puteh, Adam Sijam, Kamaruzaman Diaporthe/Phomopsis Complex (DPC) can infect soybean seed and reduce its germination in the field and also survive on the seed during storage. Ten soybean seed lots which were stored at 0°C for up to 13 years were used to evaluate the percentage of DPC infection on them and also to identify the DPC isolates. Morphological and molecular methods were used to determine the longevity and frequency of DPC isolates in stored soybeans seeds. Conventional and nested Polymerase Chain Reaction (PCR) was used for amplification of pure cultures and seed lots. The longevity of isolates in storage was found to be <9 years which suggested that Diaporthe/Phomosis sp., can survive up to 9 years in cold storage (0°C). Six isolates of DPC were detected, identified and characterized based on morphological and molecular methods in soybean seeds for the first time in Malaysia. Most of the isolates identified belonged to Phomopsis longicolla and only one isolate identified from Diaporthe Phaseolorum var. sojae. All isolates that were identified using morphological technique were confirmed using molecular method and registered in national center of biotechnology information (NCBI). The result of this study showed that DPC isolates can survive for long time in storage. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23794/2/First%20report%20of%20Diaporthe.pdf Raeisi, Samiyeh and Puteh, Adam and Sijam, Kamaruzaman (2011) First report of Diaporthe/Phomopsis complex isolates in soybean from Malaysia and their longevity in stored seeds. Asian Journal of Plant Pathology, 5 (4). pp. 146-154. ISSN 1819-1541 10.3923/ajppaj.2011.146.154 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Diaporthe/Phomopsis Complex (DPC) can infect soybean seed and reduce its germination in the field and also survive on the seed during storage. Ten soybean seed lots which were stored at 0°C for up to 13 years were used to evaluate the percentage of DPC infection on them and also to identify the DPC isolates. Morphological and molecular methods were used to determine the longevity and frequency of DPC isolates in stored soybeans seeds. Conventional and nested Polymerase Chain Reaction (PCR) was used for amplification of pure cultures and seed lots. The longevity of isolates in storage was found to be <9 years which suggested that Diaporthe/Phomosis sp., can survive up to 9 years in cold storage (0°C). Six isolates of DPC were detected, identified and characterized based on morphological and molecular methods in soybean seeds for the first time in Malaysia. Most of the isolates identified belonged to Phomopsis longicolla and only one isolate identified from Diaporthe Phaseolorum var. sojae. All isolates that were identified using morphological technique were confirmed using molecular method and registered in national center of biotechnology information (NCBI). The result of this study showed that DPC isolates can survive for long time in storage.
format Article
author Raeisi, Samiyeh
Puteh, Adam
Sijam, Kamaruzaman
spellingShingle Raeisi, Samiyeh
Puteh, Adam
Sijam, Kamaruzaman
First report of Diaporthe/Phomopsis complex isolates in soybean from Malaysia and their longevity in stored seeds.
author_facet Raeisi, Samiyeh
Puteh, Adam
Sijam, Kamaruzaman
author_sort Raeisi, Samiyeh
title First report of Diaporthe/Phomopsis complex isolates in soybean from Malaysia and their longevity in stored seeds.
title_short First report of Diaporthe/Phomopsis complex isolates in soybean from Malaysia and their longevity in stored seeds.
title_full First report of Diaporthe/Phomopsis complex isolates in soybean from Malaysia and their longevity in stored seeds.
title_fullStr First report of Diaporthe/Phomopsis complex isolates in soybean from Malaysia and their longevity in stored seeds.
title_full_unstemmed First report of Diaporthe/Phomopsis complex isolates in soybean from Malaysia and their longevity in stored seeds.
title_sort first report of diaporthe/phomopsis complex isolates in soybean from malaysia and their longevity in stored seeds.
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/23794/2/First%20report%20of%20Diaporthe.pdf
http://psasir.upm.edu.my/id/eprint/23794/
_version_ 1643828167515832320
score 13.211869

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