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In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)

Phalaenopsis, with long arching sprays of flowers, are among the most beautiful flowers in the world. Phalaenopsis is an important genus and one of the most popular epiphytic monopodial orchids, grown commercially for the production of cut flowers and potted plants. Most of them have different and i...

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Main Authors: Niknejad, Azadeh, Abdul Kadir, Mihdzar, Kadzimin, Saleh
Format: Article
Language:English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/23770/1/23770.pdf
http://psasir.upm.edu.my/id/eprint/23770/
http://www.academicjournals.org/journal/AJB/article-abstract/BBDEBF635675
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spelling my.upm.eprints.237702017-11-14T08:34:15Z http://psasir.upm.edu.my/id/eprint/23770/ In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae) Niknejad, Azadeh Abdul Kadir, Mihdzar Kadzimin, Saleh Phalaenopsis, with long arching sprays of flowers, are among the most beautiful flowers in the world. Phalaenopsis is an important genus and one of the most popular epiphytic monopodial orchids, grown commercially for the production of cut flowers and potted plants. Most of them have different and interesting morphological characteristics which have different value to the breeders. Phalaenopsis gigantea is one of the most difficult to grow and has the potential of producing beautiful hybrids. An efficient and reproducible method for large-scale propagation of Ph. giganteausing leaf sections has been developed. Leaf sections from in vitro young plants were cultured on New Dogashima Medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mg/L) alone and in combinations with (auxins a-naphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mg/L). The explants developed calli and protocorm-like-bodies (PLBs) within 6 weeks of culture. Treatment TDZ in combination with auxins was found to be the best for the induction of callus and PLBs. In vitroregeneration of Ph. gigantea PLB was achieved by exposure to light and transferring to hormone free NDM solid medium. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23770/1/23770.pdf Niknejad, Azadeh and Abdul Kadir, Mihdzar and Kadzimin, Saleh (2011) In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae). African Journal of Biotechnology, 10 (56). art. no. BBDEBF635675. pp. 11808-11816. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/BBDEBF635675
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Phalaenopsis, with long arching sprays of flowers, are among the most beautiful flowers in the world. Phalaenopsis is an important genus and one of the most popular epiphytic monopodial orchids, grown commercially for the production of cut flowers and potted plants. Most of them have different and interesting morphological characteristics which have different value to the breeders. Phalaenopsis gigantea is one of the most difficult to grow and has the potential of producing beautiful hybrids. An efficient and reproducible method for large-scale propagation of Ph. giganteausing leaf sections has been developed. Leaf sections from in vitro young plants were cultured on New Dogashima Medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mg/L) alone and in combinations with (auxins a-naphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mg/L). The explants developed calli and protocorm-like-bodies (PLBs) within 6 weeks of culture. Treatment TDZ in combination with auxins was found to be the best for the induction of callus and PLBs. In vitroregeneration of Ph. gigantea PLB was achieved by exposure to light and transferring to hormone free NDM solid medium.
format Article
author Niknejad, Azadeh
Abdul Kadir, Mihdzar
Kadzimin, Saleh
spellingShingle Niknejad, Azadeh
Abdul Kadir, Mihdzar
Kadzimin, Saleh
In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)
author_facet Niknejad, Azadeh
Abdul Kadir, Mihdzar
Kadzimin, Saleh
author_sort Niknejad, Azadeh
title In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)
title_short In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)
title_full In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)
title_fullStr In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)
title_full_unstemmed In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)
title_sort in vitro plant regeneration from protocorms-like bodies (plbs) and callus of phalaenopsis gigantea (epidendroideae: orchidaceae)
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/23770/1/23770.pdf
http://psasir.upm.edu.my/id/eprint/23770/
http://www.academicjournals.org/journal/AJB/article-abstract/BBDEBF635675
_version_ 1643828159480594432
score 13.211869

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