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Rapid identification and differentiation of Xanthomonas oryzae pv oryzae strain with primer 16S-23S rDNA from rice fields in Peninsular Malaysia.

Primer pairs XOR-F/XOR-R2 was used for rapid identification and differentiation of thirty strains of Xanthomonas oryzae pv. oryzae (Xoo) were collected from rice fields in Penang, Kedah, Selangor and Melaka during the period from 2008 to 2010. Purified DNA was extracted using a modified CTAB method...

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Main Authors: Keshavarz, Kavous, Sijam, Kamaruzaman, Mior Ahmad, Zainal Abidin, Hashim, Habibuddin, Nazerian, Eisa
Format: Article
Language:English
English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/23723/1/Rapid%20identification%20and%20differentiation%20of%20Xanthomonas%20oryzae%20pv%20oryzae%20strain%20with%20primer%2016S.pdf
http://psasir.upm.edu.my/id/eprint/23723/
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spelling my.upm.eprints.237232015-09-22T02:49:43Z http://psasir.upm.edu.my/id/eprint/23723/ Rapid identification and differentiation of Xanthomonas oryzae pv oryzae strain with primer 16S-23S rDNA from rice fields in Peninsular Malaysia. Keshavarz, Kavous Sijam, Kamaruzaman Mior Ahmad, Zainal Abidin Hashim, Habibuddin Nazerian, Eisa Primer pairs XOR-F/XOR-R2 was used for rapid identification and differentiation of thirty strains of Xanthomonas oryzae pv. oryzae (Xoo) were collected from rice fields in Penang, Kedah, Selangor and Melaka during the period from 2008 to 2010. Purified DNA was extracted using a modified CTAB method and was used in the PCR. Four hundred and seventy bp product was amplified from DNA of 30 strain using XOR-F/XOR-R2 primer pairs. Sequence similarities of the intergenic region in the 16S-23S rDNA in the Malaysian strains were as high as 99-100%. Cluster analysis based on the sequencing shows that the strains are grouped one main cluster and four groups. The minimum role of varietal influence on strain variability is partly due to the almost homogenous planting of two popular rice varieties in Peninsular Malaysia during the period of the study. On the other hand, phylogenetic analysis by using intergenic region 16S-23S divided the strains into one main cluster and four groups. The first group is represented by isolates collected from Penang and the second groups from Selangor. The third and fourth groups represented strains collected from Melaka and Kedah, respectively. The present study confirmed that direct DNA extraction from infected rice tissue by using CTAB method, followed by PCR effective methods for the identification of Xoo. Further more the results indicated that strains differentiation may be affected by the geographical areas. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23723/1/Rapid%20identification%20and%20differentiation%20of%20Xanthomonas%20oryzae%20pv%20oryzae%20strain%20with%20primer%2016S.pdf Keshavarz, Kavous and Sijam, Kamaruzaman and Mior Ahmad, Zainal Abidin and Hashim, Habibuddin and Nazerian, Eisa (2011) Rapid identification and differentiation of Xanthomonas oryzae pv oryzae strain with primer 16S-23S rDNA from rice fields in Peninsular Malaysia. Asian Journal of Plant Pathology, 5 (2). pp. 93-99. ISSN 1819-1541 10.3923/ajppaj.2011.93.99 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Primer pairs XOR-F/XOR-R2 was used for rapid identification and differentiation of thirty strains of Xanthomonas oryzae pv. oryzae (Xoo) were collected from rice fields in Penang, Kedah, Selangor and Melaka during the period from 2008 to 2010. Purified DNA was extracted using a modified CTAB method and was used in the PCR. Four hundred and seventy bp product was amplified from DNA of 30 strain using XOR-F/XOR-R2 primer pairs. Sequence similarities of the intergenic region in the 16S-23S rDNA in the Malaysian strains were as high as 99-100%. Cluster analysis based on the sequencing shows that the strains are grouped one main cluster and four groups. The minimum role of varietal influence on strain variability is partly due to the almost homogenous planting of two popular rice varieties in Peninsular Malaysia during the period of the study. On the other hand, phylogenetic analysis by using intergenic region 16S-23S divided the strains into one main cluster and four groups. The first group is represented by isolates collected from Penang and the second groups from Selangor. The third and fourth groups represented strains collected from Melaka and Kedah, respectively. The present study confirmed that direct DNA extraction from infected rice tissue by using CTAB method, followed by PCR effective methods for the identification of Xoo. Further more the results indicated that strains differentiation may be affected by the geographical areas.
format Article
author Keshavarz, Kavous
Sijam, Kamaruzaman
Mior Ahmad, Zainal Abidin
Hashim, Habibuddin
Nazerian, Eisa
spellingShingle Keshavarz, Kavous
Sijam, Kamaruzaman
Mior Ahmad, Zainal Abidin
Hashim, Habibuddin
Nazerian, Eisa
Rapid identification and differentiation of Xanthomonas oryzae pv oryzae strain with primer 16S-23S rDNA from rice fields in Peninsular Malaysia.
author_facet Keshavarz, Kavous
Sijam, Kamaruzaman
Mior Ahmad, Zainal Abidin
Hashim, Habibuddin
Nazerian, Eisa
author_sort Keshavarz, Kavous
title Rapid identification and differentiation of Xanthomonas oryzae pv oryzae strain with primer 16S-23S rDNA from rice fields in Peninsular Malaysia.
title_short Rapid identification and differentiation of Xanthomonas oryzae pv oryzae strain with primer 16S-23S rDNA from rice fields in Peninsular Malaysia.
title_full Rapid identification and differentiation of Xanthomonas oryzae pv oryzae strain with primer 16S-23S rDNA from rice fields in Peninsular Malaysia.
title_fullStr Rapid identification and differentiation of Xanthomonas oryzae pv oryzae strain with primer 16S-23S rDNA from rice fields in Peninsular Malaysia.
title_full_unstemmed Rapid identification and differentiation of Xanthomonas oryzae pv oryzae strain with primer 16S-23S rDNA from rice fields in Peninsular Malaysia.
title_sort rapid identification and differentiation of xanthomonas oryzae pv oryzae strain with primer 16s-23s rdna from rice fields in peninsular malaysia.
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/23723/1/Rapid%20identification%20and%20differentiation%20of%20Xanthomonas%20oryzae%20pv%20oryzae%20strain%20with%20primer%2016S.pdf
http://psasir.upm.edu.my/id/eprint/23723/
_version_ 1643828145486299136
score 13.211869

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