Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin
Microsomal fractions were isolated from midguts of western spruce budworm larvae and rat liver. The microsomal preparations were then assayed for cytochrome P-450 content and aldrin epoxidase activity. Both microsomal preparations showed distinct spectrum of cytochrome P-450. Relative to protein...
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| Format: | Article |
| Language: | English |
| Published: |
Universiti Pertanian Malaysia
1985
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| Online Access: | http://psasir.upm.edu.my/id/eprint/2366/1/Extraction_of_Microsomal_Fraction_from.pdf http://psasir.upm.edu.my/id/eprint/2366/ |
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| Summary: | Microsomal fractions were isolated from midguts of western spruce budworm larvae and rat
liver. The microsomal preparations were then assayed for cytochrome P-450 content and aldrin
epoxidase activity. Both microsomal preparations showed distinct spectrum of cytochrome P-450.
Relative to protein content, the cytochrome P-450 content of midguts of western spruce budworm was
lower by 7 - 9 times compared to that of the rat liver. Epoxidase activity is present in the two microsomal
preparations as indicated by their ability to convert aldrin to dieldrin. The activity of both
microsomal preparations was inhibited by piperonyl butoxide. |
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