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Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus

The effect of medium composition and environmental condition on the production of cellulase by Aspergillus terreus was investigated using shake flask culture with oil palm empty fruit bunch (OPEFB) as substrate. The highest activity of FPase (0.76 U ml-1), CMCase (8.64 U ml-1) and β-glucosidase (6.8...

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Main Authors: Shahriarinour, Mahdi, Abdul Wahab, Mohd Noor, Mohamad, Rosfarizan, Mustafa, Shuhaimi, Ariff, Arbakariya
Format: Article
Language:English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22413/1/Effect%20of%20medium%20composition%20and%20cultural%20condition%20on.pdf
http://psasir.upm.edu.my/id/eprint/22413/
http://www.academicjournals.org/journal/AJB/article-abstract/2C68E2D33210
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spelling my.upm.eprints.224132017-11-14T02:03:17Z http://psasir.upm.edu.my/id/eprint/22413/ Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus Shahriarinour, Mahdi Abdul Wahab, Mohd Noor Mohamad, Rosfarizan Mustafa, Shuhaimi Ariff, Arbakariya The effect of medium composition and environmental condition on the production of cellulase by Aspergillus terreus was investigated using shake flask culture with oil palm empty fruit bunch (OPEFB) as substrate. The highest activity of FPase (0.76 U ml-1), CMCase (8.64 U ml-1) and β-glucosidase (6.81 U ml-1) was obtained in medium containing 6 g L-1 yeast extract and 10 g L-1 delignified OPEFB fiber. In fermentation with the addition of Tween 80 (2 ml L-1) as surfactant, the production of cellulase was increased by two-fold as compared to fermentation without surfactant. Cellulase production by A. terreus was also enhanced with the addition of calcium chloride (3 mM) and magnesium sulfate (5 mM) in the medium. Optimum pH and temperature for cellulase production by A. terreus was 5.5 and 28°C, respectively. Cellulase production in agitated shake flask fermentation at 200 rpm was four times higher as compared to fermentation in static flask. Academic Journals 2011-07-25 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22413/1/Effect%20of%20medium%20composition%20and%20cultural%20condition%20on.pdf Shahriarinour, Mahdi and Abdul Wahab, Mohd Noor and Mohamad, Rosfarizan and Mustafa, Shuhaimi and Ariff, Arbakariya (2011) Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus. African Journal of Biotechnology, 10 (38). art. no. 2C68E2D33210. pp. 7459-7467. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/2C68E2D33210
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The effect of medium composition and environmental condition on the production of cellulase by Aspergillus terreus was investigated using shake flask culture with oil palm empty fruit bunch (OPEFB) as substrate. The highest activity of FPase (0.76 U ml-1), CMCase (8.64 U ml-1) and β-glucosidase (6.81 U ml-1) was obtained in medium containing 6 g L-1 yeast extract and 10 g L-1 delignified OPEFB fiber. In fermentation with the addition of Tween 80 (2 ml L-1) as surfactant, the production of cellulase was increased by two-fold as compared to fermentation without surfactant. Cellulase production by A. terreus was also enhanced with the addition of calcium chloride (3 mM) and magnesium sulfate (5 mM) in the medium. Optimum pH and temperature for cellulase production by A. terreus was 5.5 and 28°C, respectively. Cellulase production in agitated shake flask fermentation at 200 rpm was four times higher as compared to fermentation in static flask.
format Article
author Shahriarinour, Mahdi
Abdul Wahab, Mohd Noor
Mohamad, Rosfarizan
Mustafa, Shuhaimi
Ariff, Arbakariya
spellingShingle Shahriarinour, Mahdi
Abdul Wahab, Mohd Noor
Mohamad, Rosfarizan
Mustafa, Shuhaimi
Ariff, Arbakariya
Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus
author_facet Shahriarinour, Mahdi
Abdul Wahab, Mohd Noor
Mohamad, Rosfarizan
Mustafa, Shuhaimi
Ariff, Arbakariya
author_sort Shahriarinour, Mahdi
title Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus
title_short Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus
title_full Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus
title_fullStr Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus
title_full_unstemmed Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus
title_sort effect of medium composition and cultural condition on cellulase production by aspergillus terreus
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22413/1/Effect%20of%20medium%20composition%20and%20cultural%20condition%20on.pdf
http://psasir.upm.edu.my/id/eprint/22413/
http://www.academicjournals.org/journal/AJB/article-abstract/2C68E2D33210
_version_ 1643827819933859840
score 13.211869

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