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Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues

Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensis treated with 2 mg/l of 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). To optimize the precursors to increase the prod...

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Main Authors: Masoumian, M., Ariff, Arbakariya, Ahmad, Syahida, Mahmood, Maziah
Format: Article
Language:English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22375/1/Effect%20of%20precursors%20on%20flavonoid%20production%20by.pdf
http://psasir.upm.edu.my/id/eprint/22375/
http://www.academicjournals.org/journal/AJB/article-abstract/CB9F21230655
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spelling my.upm.eprints.223752017-11-15T03:11:28Z http://psasir.upm.edu.my/id/eprint/22375/ Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues Masoumian, M. Ariff, Arbakariya Ahmad, Syahida Mahmood, Maziah Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensis treated with 2 mg/l of 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). To optimize the precursors to increase the production of flavonoid, different precursors were used. The data showed that 4 mg/l proline produced the highest flavonoid yield (10.77 ± 0.25 mg/g DW). The increase in proline concentration did not significantly increase the production of flavonoid. The highest flavonoid yield (10.59 ± 0.18 mg/g DW) was produced in 1 mg/l of glutamine. No significant increase was attained in the flavonoid yield in callus treated with 2, 3 mg/l compared with the control. Phenylalanine at the concentration of 3 mg/l, successfully triggered the production of flavonoid (11.43 ± 0.12 mg/g DW), which was 23% higher than the control. The highest flavonoid production was attained in calluses treated with 4 mg/l of naringenin; and it was 19.72% higher compared with the control. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22375/1/Effect%20of%20precursors%20on%20flavonoid%20production%20by.pdf Masoumian, M. and Ariff, Arbakariya and Ahmad, Syahida and Mahmood, Maziah (2011) Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues. African Journal of Biotechnology, 10 (32). art. no. CB9F21230655. pp. 6021-6029. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/CB9F21230655
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensis treated with 2 mg/l of 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). To optimize the precursors to increase the production of flavonoid, different precursors were used. The data showed that 4 mg/l proline produced the highest flavonoid yield (10.77 ± 0.25 mg/g DW). The increase in proline concentration did not significantly increase the production of flavonoid. The highest flavonoid yield (10.59 ± 0.18 mg/g DW) was produced in 1 mg/l of glutamine. No significant increase was attained in the flavonoid yield in callus treated with 2, 3 mg/l compared with the control. Phenylalanine at the concentration of 3 mg/l, successfully triggered the production of flavonoid (11.43 ± 0.12 mg/g DW), which was 23% higher than the control. The highest flavonoid production was attained in calluses treated with 4 mg/l of naringenin; and it was 19.72% higher compared with the control.
format Article
author Masoumian, M.
Ariff, Arbakariya
Ahmad, Syahida
Mahmood, Maziah
spellingShingle Masoumian, M.
Ariff, Arbakariya
Ahmad, Syahida
Mahmood, Maziah
Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues
author_facet Masoumian, M.
Ariff, Arbakariya
Ahmad, Syahida
Mahmood, Maziah
author_sort Masoumian, M.
title Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues
title_short Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues
title_full Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues
title_fullStr Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues
title_full_unstemmed Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues
title_sort effect of precursors on flavonoid production by hydrocotyle bonariensis callus tissues
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22375/1/Effect%20of%20precursors%20on%20flavonoid%20production%20by.pdf
http://psasir.upm.edu.my/id/eprint/22375/
http://www.academicjournals.org/journal/AJB/article-abstract/CB9F21230655
_version_ 1643827809823490048
score 13.211869

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