Effect of retention time on biohydrogen production by microbial consortia immbolised in polydimethylsiloxane
An investigation on biohydrogen production from palm oil mill effluent (POME) was conducted in a continuous stirred tank reactor seeded with polydimethyl-siloxane (PDMS) immobilised mixed cultures at adjusted retention time. The hydrogen-producing bacteria obtained from an anaerobic digester used fo...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22361/1/Effect%20of%20retention%20time%20on%20biohydrogen%20production%20by%20microbial%20consortia%20immbolised%20in%20polydimethylsiloxane.pdf http://psasir.upm.edu.my/id/eprint/22361/ http://www.academicjournals.org/journal/AJB/article-abstract/C7081B027236 |
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| Summary: | An investigation on biohydrogen production from palm oil mill effluent (POME) was conducted in a continuous stirred tank reactor seeded with polydimethyl-siloxane (PDMS) immobilised mixed cultures at adjusted retention time. The hydrogen-producing bacteria obtained from an anaerobic digester used for treating POME were acclimatised and immobilised in PDMS. The immobilised cultures were assessed for their effectiveness in generating hydrogen in a continuous system. In this study, the PDMS cultures were fed with raw POME at hydraulic retention times of 6, 4 and 2 days and operated at controlled pH and temperature of 5.5 and 55°C, respectively. At hydraulic retention time (HRT) 2 days, the average hydrogen production rate per unit volume of POME was 2.1 NL/L/d. Hydrogen constituted up to 43% of the total gas produced and methane was not detected throughout the 150 days of continuous operation. The soluble carbohydrate degradation efficiency was highest at 81.2% during HRT 4 days and the concentration of soluble metabolites produced, followed the order of acetic > butyric > ethanol > propionic acid. The microbial diversity of the immobilised consortia determined by denaturing gradient gel electrophoresis (DGGE) changed at different HRTs; with increasing dominant species phylogenetically related to Clostridaceae. |
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