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Production of long helical capsid of Nipah virus by Pichia pastoris.

The nucleocapsid (N) protein of Nipah virus (NiV) produced in a recombinant host can replace the use of inactivated virus as a diagnostic reagent because it is safer and affordable. The aim of this study was to express the N protein in Pichia pastoris. The N gene of NiV was cloned into the yeast exp...

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Main Authors: Joseph, Narcisse M. S., Tey, Beng Ti, Tan, Chon Seng, Shafee, Norazizah, Tan, Wen Siang
Format: Article
Language:English
English
Published: Elsevier 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22301/1/Production%20of%20long%20helical%20capsid%20of%20Nipah%20virus%20by%20Pichia%20pastoris.pdf
http://psasir.upm.edu.my/id/eprint/22301/
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spelling my.upm.eprints.223012015-10-06T02:12:12Z http://psasir.upm.edu.my/id/eprint/22301/ Production of long helical capsid of Nipah virus by Pichia pastoris. Joseph, Narcisse M. S. Tey, Beng Ti Tan, Chon Seng Shafee, Norazizah Tan, Wen Siang The nucleocapsid (N) protein of Nipah virus (NiV) produced in a recombinant host can replace the use of inactivated virus as a diagnostic reagent because it is safer and affordable. The aim of this study was to express the N protein in Pichia pastoris. The N gene of NiV was cloned into the yeast expression vector, pPICZ B and expressed in P. pastoris. The recombinant N protein of NiV was purified using sucrose density gradient ultracentrifugation and was confirmed with Western blotting using rabbit anti-N antibody. The P. pastoris expressed N protein self-assembled into helical structures as large as 1.5 μm as shown in an electron micrograph. ELISA analysis performed with the swine sera obtained during the viral outbreak proved that the recombinant N protein to be highly antigenic. The NiV N protein produced in P. pastoris serves as an alternative to the recombinant N protein produced in Escherichia coli. Elsevier 2011-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22301/1/Production%20of%20long%20helical%20capsid%20of%20Nipah%20virus%20by%20Pichia%20pastoris.pdf Joseph, Narcisse M. S. and Tey, Beng Ti and Tan, Chon Seng and Shafee, Norazizah and Tan, Wen Siang (2011) Production of long helical capsid of Nipah virus by Pichia pastoris. Process Biochemistry, 46 (9). pp. 1871-1874. ISSN 1359-5113; ESSN: 1873-3298 10.1016/j.procbio.2011.06.004 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The nucleocapsid (N) protein of Nipah virus (NiV) produced in a recombinant host can replace the use of inactivated virus as a diagnostic reagent because it is safer and affordable. The aim of this study was to express the N protein in Pichia pastoris. The N gene of NiV was cloned into the yeast expression vector, pPICZ B and expressed in P. pastoris. The recombinant N protein of NiV was purified using sucrose density gradient ultracentrifugation and was confirmed with Western blotting using rabbit anti-N antibody. The P. pastoris expressed N protein self-assembled into helical structures as large as 1.5 μm as shown in an electron micrograph. ELISA analysis performed with the swine sera obtained during the viral outbreak proved that the recombinant N protein to be highly antigenic. The NiV N protein produced in P. pastoris serves as an alternative to the recombinant N protein produced in Escherichia coli.
format Article
author Joseph, Narcisse M. S.
Tey, Beng Ti
Tan, Chon Seng
Shafee, Norazizah
Tan, Wen Siang
spellingShingle Joseph, Narcisse M. S.
Tey, Beng Ti
Tan, Chon Seng
Shafee, Norazizah
Tan, Wen Siang
Production of long helical capsid of Nipah virus by Pichia pastoris.
author_facet Joseph, Narcisse M. S.
Tey, Beng Ti
Tan, Chon Seng
Shafee, Norazizah
Tan, Wen Siang
author_sort Joseph, Narcisse M. S.
title Production of long helical capsid of Nipah virus by Pichia pastoris.
title_short Production of long helical capsid of Nipah virus by Pichia pastoris.
title_full Production of long helical capsid of Nipah virus by Pichia pastoris.
title_fullStr Production of long helical capsid of Nipah virus by Pichia pastoris.
title_full_unstemmed Production of long helical capsid of Nipah virus by Pichia pastoris.
title_sort production of long helical capsid of nipah virus by pichia pastoris.
publisher Elsevier
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22301/1/Production%20of%20long%20helical%20capsid%20of%20Nipah%20virus%20by%20Pichia%20pastoris.pdf
http://psasir.upm.edu.my/id/eprint/22301/
_version_ 1643827788235407360
score 13.211869

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