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Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system.

The partitioning and recovery of lipase derived from Burkholderia sp. ST8 strain was explored using temperature-induced aqueous micellar two-phase system (AMTPS) composed of single nonionic surfactant. Nonionic surfactant Triton X-114 and Pluronic series (triblock copolymer) were evaluated in terms...

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Main Authors: Chien, Wei Ooi, Chin, Ping Tan, Siew, Ling Hii, Ariff, Arbakariya, Ibrahim, Shaliza, Tau, Chuan Ling
Format: Article
Language:English
English
Published: Elsevier 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22281/1/Primary%20recovery%20of%20lipase%20derived%20from%20Burkholderia%20sp.pdf
http://psasir.upm.edu.my/id/eprint/22281/
http://www.elsevier.com/
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spelling my.upm.eprints.222812015-10-16T07:25:12Z http://psasir.upm.edu.my/id/eprint/22281/ Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system. Chien, Wei Ooi Chin, Ping Tan Siew, Ling Hii Ariff, Arbakariya Ibrahim, Shaliza Tau, Chuan Ling The partitioning and recovery of lipase derived from Burkholderia sp. ST8 strain was explored using temperature-induced aqueous micellar two-phase system (AMTPS) composed of single nonionic surfactant. Nonionic surfactant Triton X-114 and Pluronic series (triblock copolymer) were evaluated in terms of their clouding phenomenon (cloud-point temperature) and the performance of the lipase partitioning in these AMTPSs. Pluronic L81 showed the most optimum partition efficiency for the recovery of lipase to the micellar phase of the AMTPS. Based on the AMTPS which consisted of 24% (w/w) Pluronic L81 and 0.5% (w/w) potassium chloride (KCl), the selectivity of lipase partitioned to bottom phase has been enhanced to 0.035 and the lipase was purified 7.2 fold. Furthermore, the lipase from the micellar phase was consecutively extracted to a new aqueous solution, with an aim of removing the surfactant from the purified lipase. It was attained by replacing the aqueous top phase from the primary recovery of AMTPS with a new potassium thiocyanate (KSCN) solution. The lipase was then recovered in the newly formed bottom aqueous phase which culminated in the yield of 89% and partition coefficients of 0.34 and 4.50 for lipase and surfactant, respectively. AMTPS offers a convenient and efficient method for the primary recovery of lipase with low cost, large loading capacity and the potential of linear scale up. Elsevier 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22281/1/Primary%20recovery%20of%20lipase%20derived%20from%20Burkholderia%20sp.pdf Chien, Wei Ooi and Chin, Ping Tan and Siew, Ling Hii and Ariff, Arbakariya and Ibrahim, Shaliza and Tau, Chuan Ling (2011) Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system. Process Biochemistry, 46 (9). pp. 1847-1852. ISSN 1359-5113 http://www.elsevier.com/ 10.1016/j.procbio.2011.06.014 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The partitioning and recovery of lipase derived from Burkholderia sp. ST8 strain was explored using temperature-induced aqueous micellar two-phase system (AMTPS) composed of single nonionic surfactant. Nonionic surfactant Triton X-114 and Pluronic series (triblock copolymer) were evaluated in terms of their clouding phenomenon (cloud-point temperature) and the performance of the lipase partitioning in these AMTPSs. Pluronic L81 showed the most optimum partition efficiency for the recovery of lipase to the micellar phase of the AMTPS. Based on the AMTPS which consisted of 24% (w/w) Pluronic L81 and 0.5% (w/w) potassium chloride (KCl), the selectivity of lipase partitioned to bottom phase has been enhanced to 0.035 and the lipase was purified 7.2 fold. Furthermore, the lipase from the micellar phase was consecutively extracted to a new aqueous solution, with an aim of removing the surfactant from the purified lipase. It was attained by replacing the aqueous top phase from the primary recovery of AMTPS with a new potassium thiocyanate (KSCN) solution. The lipase was then recovered in the newly formed bottom aqueous phase which culminated in the yield of 89% and partition coefficients of 0.34 and 4.50 for lipase and surfactant, respectively. AMTPS offers a convenient and efficient method for the primary recovery of lipase with low cost, large loading capacity and the potential of linear scale up.
format Article
author Chien, Wei Ooi
Chin, Ping Tan
Siew, Ling Hii
Ariff, Arbakariya
Ibrahim, Shaliza
Tau, Chuan Ling
spellingShingle Chien, Wei Ooi
Chin, Ping Tan
Siew, Ling Hii
Ariff, Arbakariya
Ibrahim, Shaliza
Tau, Chuan Ling
Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system.
author_facet Chien, Wei Ooi
Chin, Ping Tan
Siew, Ling Hii
Ariff, Arbakariya
Ibrahim, Shaliza
Tau, Chuan Ling
author_sort Chien, Wei Ooi
title Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system.
title_short Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system.
title_full Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system.
title_fullStr Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system.
title_full_unstemmed Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system.
title_sort primary recovery of lipase derived from burkholderia sp. st8 with aqueous micellar two-phase system.
publisher Elsevier
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22281/1/Primary%20recovery%20of%20lipase%20derived%20from%20Burkholderia%20sp.pdf
http://psasir.upm.edu.my/id/eprint/22281/
http://www.elsevier.com/
_version_ 1643827782315147264
score 13.211869

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