Cover Image

Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress

Tissue culture technique can be used as a source for genetic variability by means of genetic modifications through the process of in vitro cultures. This technique has been widely used for breeding purposes, especially for stress tolerance selection, which severely limits rice production. Also, the...

Full description

Saved in:
Bibliographic Details
Main Authors: Htwe, New Nwe, Mahmood, Maziah, Ho, Chai Ling, Qamaruz Zaman, Faridah, Mohd Zain, Abdullah
Format: Article
Language:English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22275/1/22275.pdf
http://psasir.upm.edu.my/id/eprint/22275/
http://www.academicjournals.org/journal/AJB/article-abstract/B98ED7824807
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.22275
record_format eprints
spelling my.upm.eprints.222752017-11-10T09:17:39Z http://psasir.upm.edu.my/id/eprint/22275/ Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress Htwe, New Nwe Mahmood, Maziah Ho, Chai Ling Qamaruz Zaman, Faridah Mohd Zain, Abdullah Tissue culture technique can be used as a source for genetic variability by means of genetic modifications through the process of in vitro cultures. This technique has been widely used for breeding purposes, especially for stress tolerance selection, which severely limits rice production. Also, the establishment of a suitable plant regeneration system is a prerequisite for successful genetic transformation. The aim of this study is to identify the most suitable medium and to assess the genotype performance for in vitro salt stress responses in some selected Malaysian rice genotypes. Differences in culture conditions, growth rate, plant hormone responses and accumulation of proline content were monitored. All the selected genotypes showed that the callus-growth capacities were significantly affected by the genotypes and the culture media. Evidently, callus was best induced on the MS medium added with 10 µM dichlorophenoxyacetic acid (2,4-D) and 0.4 gm/l casein hydrolysate. In addition, the shoot regeneration capacity from the callus was the most effective in ½ MS added with 10 µM 6 benzylaminopurine (BAP). The two genotypes, that is, MR219 (line 4) and MR219 (line 9), consistently performed the best in both callus culture (93.51 and 92.22%) and plant regeneration capacity (27.03 and 26.34%), respectively. When the callus were transferred to different concentrations of NaCl (0 to 250 mM) supplemented medium in order to examine their responses to salinity, the two genotypes, that is, MR219 (line 4) and MR219 (line 9), showed a significant decline in the callus growth (18.83 and 23.5%) and regeneration capacity (7.33 and 7.68%), respectively. A similar trend was also observed for the proline content. All the genotypes significantly resulted in proline accumulation. MR211 showed the highest accumulation, whereas MR219 (line 4) revealed the lowest proline accumulation. These proline content analyses further suggest potential salinity tolerance in the rice genotypes. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22275/1/22275.pdf Htwe, New Nwe and Mahmood, Maziah and Ho, Chai Ling and Qamaruz Zaman, Faridah and Mohd Zain, Abdullah (2011) Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress. African Journal of Biotechnology, 10 (3). art. no. B98ED7824807. pp. 350-362. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/B98ED7824807
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Tissue culture technique can be used as a source for genetic variability by means of genetic modifications through the process of in vitro cultures. This technique has been widely used for breeding purposes, especially for stress tolerance selection, which severely limits rice production. Also, the establishment of a suitable plant regeneration system is a prerequisite for successful genetic transformation. The aim of this study is to identify the most suitable medium and to assess the genotype performance for in vitro salt stress responses in some selected Malaysian rice genotypes. Differences in culture conditions, growth rate, plant hormone responses and accumulation of proline content were monitored. All the selected genotypes showed that the callus-growth capacities were significantly affected by the genotypes and the culture media. Evidently, callus was best induced on the MS medium added with 10 µM dichlorophenoxyacetic acid (2,4-D) and 0.4 gm/l casein hydrolysate. In addition, the shoot regeneration capacity from the callus was the most effective in ½ MS added with 10 µM 6 benzylaminopurine (BAP). The two genotypes, that is, MR219 (line 4) and MR219 (line 9), consistently performed the best in both callus culture (93.51 and 92.22%) and plant regeneration capacity (27.03 and 26.34%), respectively. When the callus were transferred to different concentrations of NaCl (0 to 250 mM) supplemented medium in order to examine their responses to salinity, the two genotypes, that is, MR219 (line 4) and MR219 (line 9), showed a significant decline in the callus growth (18.83 and 23.5%) and regeneration capacity (7.33 and 7.68%), respectively. A similar trend was also observed for the proline content. All the genotypes significantly resulted in proline accumulation. MR211 showed the highest accumulation, whereas MR219 (line 4) revealed the lowest proline accumulation. These proline content analyses further suggest potential salinity tolerance in the rice genotypes.
format Article
author Htwe, New Nwe
Mahmood, Maziah
Ho, Chai Ling
Qamaruz Zaman, Faridah
Mohd Zain, Abdullah
spellingShingle Htwe, New Nwe
Mahmood, Maziah
Ho, Chai Ling
Qamaruz Zaman, Faridah
Mohd Zain, Abdullah
Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress
author_facet Htwe, New Nwe
Mahmood, Maziah
Ho, Chai Ling
Qamaruz Zaman, Faridah
Mohd Zain, Abdullah
author_sort Htwe, New Nwe
title Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress
title_short Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress
title_full Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress
title_fullStr Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress
title_full_unstemmed Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress
title_sort responses of some selected malaysian rice genotypes to callus induction under in vitro salt stress
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22275/1/22275.pdf
http://psasir.upm.edu.my/id/eprint/22275/
http://www.academicjournals.org/journal/AJB/article-abstract/B98ED7824807
_version_ 1643827780472799232
score 13.211869

Similar Items