Cover Image

Effects of condensed tannins from Leucaena on methane reduction, rumen fermentation and populations of methaogens and protozoa in vitro

Different levels of purified condensed tannins (CT) extracted from Leucaena leucocephala hybrid-Rendang (LLR) were investigated for their effects on CH4 production, rumen fermentation parameters such as pH, dry matter (DM) degradability, N disappearance and volatile fatty acid (VFA) concentrations,...

Full description

Saved in:
Bibliographic Details
Main Authors: Tan, Hui Yin, Sieo, Chin Chin, Abdullah, Norhani, Liang, Juan Boo, Huang, Xiao Dan, Ho, Yin Wan
Format: Article
Language:English
Published: Elsevier 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22231/1/Effects%20of%20condensed%20tannins%20from%20Leucaena%20on%20methane%20reduction.pdf
http://psasir.upm.edu.my/id/eprint/22231/
http://www.sciencedirect.com/science/article/pii/S0377840111003178
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.22231
record_format eprints
spelling my.upm.eprints.222312016-01-12T01:45:40Z http://psasir.upm.edu.my/id/eprint/22231/ Effects of condensed tannins from Leucaena on methane reduction, rumen fermentation and populations of methaogens and protozoa in vitro Tan, Hui Yin Sieo, Chin Chin Abdullah, Norhani Liang, Juan Boo Huang, Xiao Dan Ho, Yin Wan Different levels of purified condensed tannins (CT) extracted from Leucaena leucocephala hybrid-Rendang (LLR) were investigated for their effects on CH4 production, rumen fermentation parameters such as pH, dry matter (DM) degradability, N disappearance and volatile fatty acid (VFA) concentrations, as well as on populations of rumen methanogenic archaea and protozoa in vitro. Purified CT concentrations of 0 (control), 10, 15, 20, 25 and 30 mg, and 500 mg of oven dried guinea grass (Panicum maximum) with 40 ml of buffered rumen fluid were incubated for 24 h using an in vitro gas production procedure. Total gas (ml/g DM) decreased at a decreasing rate (linear P < 0.01; quadratic P < 0.05) with increased levels of CT inclusion. CH4 production (ml/g DM) decreased at a decreasing rate (linear P < 0.01; quadratic P < 0.01) with increasing levels of CT. Total VFA concentration (mmol/L) decreased at a decreasing rate (linear P < 0.01; quadratic P < 0.01) with increasing CT inclusions. In vitro DM degradation and N disappearance declined linearly (P < 0.01) with increasing levels of CT. Estimates of rumen methanogenic archaea and protozoa populations using microbiological methods and real-time PCR assay showed linear reductions in total methanogens (P < 0.01) and total protozoa (P < 0.01) with increasing levels of CT. Methanogens in the order Methanobacteriales also declined, but with quadratic and cubic aspects. Results suggest that CT from LLR at a relatively low level of 15 mg of CT/500 mg DM reduce CH4 production by 47%, with only 7% reduction in degradation of feed DM. However, higher CT inclusions, while further reducing CH4 emissions, have substantive negative effects on DM digestibility. Elsevier 2011-11-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22231/1/Effects%20of%20condensed%20tannins%20from%20Leucaena%20on%20methane%20reduction.pdf Tan, Hui Yin and Sieo, Chin Chin and Abdullah, Norhani and Liang, Juan Boo and Huang, Xiao Dan and Ho, Yin Wan (2011) Effects of condensed tannins from Leucaena on methane reduction, rumen fermentation and populations of methaogens and protozoa in vitro. Animal Feed Science and Technology, 169 (3-4). pp. 185-193. ISSN 0337-8401; ESSN: 1873-2216 http://www.sciencedirect.com/science/article/pii/S0377840111003178 10.1016/j.anifeedsci.2011.07.004
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Different levels of purified condensed tannins (CT) extracted from Leucaena leucocephala hybrid-Rendang (LLR) were investigated for their effects on CH4 production, rumen fermentation parameters such as pH, dry matter (DM) degradability, N disappearance and volatile fatty acid (VFA) concentrations, as well as on populations of rumen methanogenic archaea and protozoa in vitro. Purified CT concentrations of 0 (control), 10, 15, 20, 25 and 30 mg, and 500 mg of oven dried guinea grass (Panicum maximum) with 40 ml of buffered rumen fluid were incubated for 24 h using an in vitro gas production procedure. Total gas (ml/g DM) decreased at a decreasing rate (linear P < 0.01; quadratic P < 0.05) with increased levels of CT inclusion. CH4 production (ml/g DM) decreased at a decreasing rate (linear P < 0.01; quadratic P < 0.01) with increasing levels of CT. Total VFA concentration (mmol/L) decreased at a decreasing rate (linear P < 0.01; quadratic P < 0.01) with increasing CT inclusions. In vitro DM degradation and N disappearance declined linearly (P < 0.01) with increasing levels of CT. Estimates of rumen methanogenic archaea and protozoa populations using microbiological methods and real-time PCR assay showed linear reductions in total methanogens (P < 0.01) and total protozoa (P < 0.01) with increasing levels of CT. Methanogens in the order Methanobacteriales also declined, but with quadratic and cubic aspects. Results suggest that CT from LLR at a relatively low level of 15 mg of CT/500 mg DM reduce CH4 production by 47%, with only 7% reduction in degradation of feed DM. However, higher CT inclusions, while further reducing CH4 emissions, have substantive negative effects on DM digestibility.
format Article
author Tan, Hui Yin
Sieo, Chin Chin
Abdullah, Norhani
Liang, Juan Boo
Huang, Xiao Dan
Ho, Yin Wan
spellingShingle Tan, Hui Yin
Sieo, Chin Chin
Abdullah, Norhani
Liang, Juan Boo
Huang, Xiao Dan
Ho, Yin Wan
Effects of condensed tannins from Leucaena on methane reduction, rumen fermentation and populations of methaogens and protozoa in vitro
author_facet Tan, Hui Yin
Sieo, Chin Chin
Abdullah, Norhani
Liang, Juan Boo
Huang, Xiao Dan
Ho, Yin Wan
author_sort Tan, Hui Yin
title Effects of condensed tannins from Leucaena on methane reduction, rumen fermentation and populations of methaogens and protozoa in vitro
title_short Effects of condensed tannins from Leucaena on methane reduction, rumen fermentation and populations of methaogens and protozoa in vitro
title_full Effects of condensed tannins from Leucaena on methane reduction, rumen fermentation and populations of methaogens and protozoa in vitro
title_fullStr Effects of condensed tannins from Leucaena on methane reduction, rumen fermentation and populations of methaogens and protozoa in vitro
title_full_unstemmed Effects of condensed tannins from Leucaena on methane reduction, rumen fermentation and populations of methaogens and protozoa in vitro
title_sort effects of condensed tannins from leucaena on methane reduction, rumen fermentation and populations of methaogens and protozoa in vitro
publisher Elsevier
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22231/1/Effects%20of%20condensed%20tannins%20from%20Leucaena%20on%20methane%20reduction.pdf
http://psasir.upm.edu.my/id/eprint/22231/
http://www.sciencedirect.com/science/article/pii/S0377840111003178
_version_ 1643827767399153664
score 13.211869

Similar Items