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Molecular identification and phylogenetic analysis of Pseudoperonospora cubensis isolates in Peninsula Malaysia

Thirteen isolates of Pseudoperonospora cubensis, the causal agent of downy mildew, were collected from cucurbit fields in five states of the western part of Peninsular Malaysia during its growing season between November 2008 and March 2009. The host range of these isolates was determined previously...

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Main Authors: Salati, Mansour, Meon, Sariah, Tan, Yee How, Wong, M.
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2012
Online Access:http://psasir.upm.edu.my/id/eprint/20948/1/84.%20Molecular%20Identification%20and%20Phylogenetic%20Analysis%20of.pdf
http://psasir.upm.edu.my/id/eprint/20948/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(S)%20Dec.%202012/08_page%2079-84.pdf
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spelling my.upm.eprints.209482015-11-24T07:01:56Z http://psasir.upm.edu.my/id/eprint/20948/ Molecular identification and phylogenetic analysis of Pseudoperonospora cubensis isolates in Peninsula Malaysia Salati, Mansour Meon, Sariah Tan, Yee How Wong, M. Thirteen isolates of Pseudoperonospora cubensis, the causal agent of downy mildew, were collected from cucurbit fields in five states of the western part of Peninsular Malaysia during its growing season between November 2008 and March 2009. The host range of these isolates was determined previously using leaf disc assay and the results indicated that there were 12 pathotypes among these isolates. The objective of this study was to analyze the 13 isolates for phylogenetic relationship using internal transcribed spacers (ITS) of ribosomal DNA (rDNA) and mitochondrial COX-II regions. A high sequence similarity among the 13 isolates and similar sequences from GenBank were detected in ITS (>99%) and COX-II (>98%) regions. Phylogenetic analysis of the 13 isolates based on Minimum Evolution method performed on ITS and COX-II regions revealed five and three groupings, respectively. However, no relationship was found between the phylogenetic groupings using both genes and pathotypes in this study. Universiti Putra Malaysia Press 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/20948/1/84.%20Molecular%20Identification%20and%20Phylogenetic%20Analysis%20of.pdf Salati, Mansour and Meon, Sariah and Tan, Yee How and Wong, M. (2012) Molecular identification and phylogenetic analysis of Pseudoperonospora cubensis isolates in Peninsula Malaysia. Pertanika Journal of Tropical Agricultural Science, 35 (S). pp. 79-84. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(S)%20Dec.%202012/08_page%2079-84.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Thirteen isolates of Pseudoperonospora cubensis, the causal agent of downy mildew, were collected from cucurbit fields in five states of the western part of Peninsular Malaysia during its growing season between November 2008 and March 2009. The host range of these isolates was determined previously using leaf disc assay and the results indicated that there were 12 pathotypes among these isolates. The objective of this study was to analyze the 13 isolates for phylogenetic relationship using internal transcribed spacers (ITS) of ribosomal DNA (rDNA) and mitochondrial COX-II regions. A high sequence similarity among the 13 isolates and similar sequences from GenBank were detected in ITS (>99%) and COX-II (>98%) regions. Phylogenetic analysis of the 13 isolates based on Minimum Evolution method performed on ITS and COX-II regions revealed five and three groupings, respectively. However, no relationship was found between the phylogenetic groupings using both genes and pathotypes in this study.
format Article
author Salati, Mansour
Meon, Sariah
Tan, Yee How
Wong, M.
spellingShingle Salati, Mansour
Meon, Sariah
Tan, Yee How
Wong, M.
Molecular identification and phylogenetic analysis of Pseudoperonospora cubensis isolates in Peninsula Malaysia
author_facet Salati, Mansour
Meon, Sariah
Tan, Yee How
Wong, M.
author_sort Salati, Mansour
title Molecular identification and phylogenetic analysis of Pseudoperonospora cubensis isolates in Peninsula Malaysia
title_short Molecular identification and phylogenetic analysis of Pseudoperonospora cubensis isolates in Peninsula Malaysia
title_full Molecular identification and phylogenetic analysis of Pseudoperonospora cubensis isolates in Peninsula Malaysia
title_fullStr Molecular identification and phylogenetic analysis of Pseudoperonospora cubensis isolates in Peninsula Malaysia
title_full_unstemmed Molecular identification and phylogenetic analysis of Pseudoperonospora cubensis isolates in Peninsula Malaysia
title_sort molecular identification and phylogenetic analysis of pseudoperonospora cubensis isolates in peninsula malaysia
publisher Universiti Putra Malaysia Press
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/20948/1/84.%20Molecular%20Identification%20and%20Phylogenetic%20Analysis%20of.pdf
http://psasir.upm.edu.my/id/eprint/20948/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(S)%20Dec.%202012/08_page%2079-84.pdf
_version_ 1643827431764656128
score 13.211869

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