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Enzyme and microbial technology: biotechnology of fats and oils.

To develop an enzyme technology industry, a sizeable enzyme bank is a priority requirement. A group of enzymes of interest to us and currently attracting attention of researchers throughout the world are the lipases. Apart from their usual hydrolytic properties, these enzymes can perform syntheti...

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Main Authors: Salleh, Abu Bakar, Abd. Razak, Che Nyonya, Wan Yunus, Wan Md. Zin, Ampon, Kamaruzaman, Basri, Mahiran
Format: Conference or Workshop Item
Language:English
English
Published: 1991
Online Access:http://psasir.upm.edu.my/id/eprint/20856/1/ID%2020856.pdf
http://psasir.upm.edu.my/id/eprint/20856/
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spelling my.upm.eprints.208562014-08-26T08:50:40Z http://psasir.upm.edu.my/id/eprint/20856/ Enzyme and microbial technology: biotechnology of fats and oils. Salleh, Abu Bakar Abd. Razak, Che Nyonya Wan Yunus, Wan Md. Zin Ampon, Kamaruzaman Basri, Mahiran To develop an enzyme technology industry, a sizeable enzyme bank is a priority requirement. A group of enzymes of interest to us and currently attracting attention of researchers throughout the world are the lipases. Apart from their usual hydrolytic properties, these enzymes can perform synthetic reactions under controlled conditions. Our group have meticulously carried out a screening programme for microbes that can produce these enzymes. So far we have isolated over 50 microbial isolates exhibiting lipolytic activities on plate and managed to study in detail 3 fungi and 2 bacteria. To improve the versatility of these enzymes we are redesigning the enzymes with the hope of obtaining an analogue with better solubility in organic solvents. We have covalently attached aldehydes of various sizes and hydrophobicity onto the enzyme lipase, and the enzyme thus derivatised showed. enhanced catalytic activity and thermal stability compared with the native unmodified enzyme. Another approach is to immobilise the enzyme. The enzyme molecule is still, in principle, to another molecule. In most cases, the molecule attached yo the enzyme is usually insoluble, hence confering the derivatised enzyme a new property of insolubility. We have shown that enzymes can be immobilised by simple adsorption onto readily available resins. 1991 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/20856/1/ID%2020856.pdf Salleh, Abu Bakar and Abd. Razak, Che Nyonya and Wan Yunus, Wan Md. Zin and Ampon, Kamaruzaman and Basri, Mahiran (1991) Enzyme and microbial technology: biotechnology of fats and oils. In: Seminar Kebangsaan Menilai Pencapaian Penyelidikan IRPA Dalam Rancangan Malaysia ke-5 Sektor Perindustrian (20-24 Disember 1991) hosted in Universiti Utara Malaysia, Kedah, 20-24 Disember 1991, Universiti Utara Malaysia, Kedah. . English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description To develop an enzyme technology industry, a sizeable enzyme bank is a priority requirement. A group of enzymes of interest to us and currently attracting attention of researchers throughout the world are the lipases. Apart from their usual hydrolytic properties, these enzymes can perform synthetic reactions under controlled conditions. Our group have meticulously carried out a screening programme for microbes that can produce these enzymes. So far we have isolated over 50 microbial isolates exhibiting lipolytic activities on plate and managed to study in detail 3 fungi and 2 bacteria. To improve the versatility of these enzymes we are redesigning the enzymes with the hope of obtaining an analogue with better solubility in organic solvents. We have covalently attached aldehydes of various sizes and hydrophobicity onto the enzyme lipase, and the enzyme thus derivatised showed. enhanced catalytic activity and thermal stability compared with the native unmodified enzyme. Another approach is to immobilise the enzyme. The enzyme molecule is still, in principle, to another molecule. In most cases, the molecule attached yo the enzyme is usually insoluble, hence confering the derivatised enzyme a new property of insolubility. We have shown that enzymes can be immobilised by simple adsorption onto readily available resins.
format Conference or Workshop Item
author Salleh, Abu Bakar
Abd. Razak, Che Nyonya
Wan Yunus, Wan Md. Zin
Ampon, Kamaruzaman
Basri, Mahiran
spellingShingle Salleh, Abu Bakar
Abd. Razak, Che Nyonya
Wan Yunus, Wan Md. Zin
Ampon, Kamaruzaman
Basri, Mahiran
Enzyme and microbial technology: biotechnology of fats and oils.
author_facet Salleh, Abu Bakar
Abd. Razak, Che Nyonya
Wan Yunus, Wan Md. Zin
Ampon, Kamaruzaman
Basri, Mahiran
author_sort Salleh, Abu Bakar
title Enzyme and microbial technology: biotechnology of fats and oils.
title_short Enzyme and microbial technology: biotechnology of fats and oils.
title_full Enzyme and microbial technology: biotechnology of fats and oils.
title_fullStr Enzyme and microbial technology: biotechnology of fats and oils.
title_full_unstemmed Enzyme and microbial technology: biotechnology of fats and oils.
title_sort enzyme and microbial technology: biotechnology of fats and oils.
publishDate 1991
url http://psasir.upm.edu.my/id/eprint/20856/1/ID%2020856.pdf
http://psasir.upm.edu.my/id/eprint/20856/
_version_ 1643827410535186432
score 13.211869

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