Cover Image

Cytotoxic and Antiproliferative Properties of Metabolites Produced by Six Strains of Lactobacillus Plantarum on Human Cancer Cells

Whole cells, cytoplasmic fractions and fermented products of LAB have been tested for anticancer effect. However, limited information is available for the metabolites produced by Lactobacillus plantarum. In this study, the anticancer effect of metabolites produced by six strains of L. plantarum (UL4...

Full description

Saved in:
Bibliographic Details
Main Author: Chuah, Li Oon
Format: Thesis
Language:English
English
Published: 2010
Online Access:http://psasir.upm.edu.my/id/eprint/19433/1/FBSB_2010_18_F.pdf
http://psasir.upm.edu.my/id/eprint/19433/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.19433
record_format eprints
spelling my.upm.eprints.194332013-05-27T08:02:21Z http://psasir.upm.edu.my/id/eprint/19433/ Cytotoxic and Antiproliferative Properties of Metabolites Produced by Six Strains of Lactobacillus Plantarum on Human Cancer Cells Chuah, Li Oon Whole cells, cytoplasmic fractions and fermented products of LAB have been tested for anticancer effect. However, limited information is available for the metabolites produced by Lactobacillus plantarum. In this study, the anticancer effect of metabolites produced by six strains of L. plantarum (UL4, TL1, RS5, RG14, RG11 and RI11) isolated from Malaysian fermented foods was evaluated. All metabolites exhibited in vitro cytotoxic effect on the tested cancer cells (breast, colorectal, cervical, liver and leukemia cancer cell lines). An increased cytotoxic effect was observed with increased dose of metabolites used and time of incubation. In particular, metabolites UL4 exerted the most potent cytotoxicity against human breast carcinoma cells MCF-7 in a dose- and time-dependent manner in MTT assay, with inhibition concentration of 50 % growth (IC50) value of 15, 12 and 10% (v/v) for 24, 48 and 72 hours of incubation, respectively. In contrast, no cytotoxicity was detected in primary human peripheral blood mononuclear cells, mouse splenocytes, thymocytes and bone marrow cells for all the six metabolites tested. However, limited cytotoxicity was detected in nonmalignant human glandular epithelium cells MCF-10A when treated with UL4 and RG14 metabolites. Additionally, UL4 metabolites did not cause haemolysis, indicating cytotoxic effect of metabolites of six strains of L. plantarum is selective for malignant cells but spared on normal cells. Antiproliferative effect was focused on MCF-7 and colon cancer cell line (HT-29). In BrdU cell proliferation assay, all tested metabolites inhibited DNA synthesis of MCF-7 and HT-29 cells. An increased antiproliferative effect was observed with increased dose of metabolites used and time of incubation. In particular, UL4 metabolites exhibited 100% proliferation inhibition on MCF-7, whereas RG14 metabolites exhibited 89% proliferation inhibition on HT-29 for 72 hours of incubation. Growth arrest study showed significant cell growth inhibition (P < 0.05) in MCF-7 treated with UL4 metabolites and HT-29 cells treated with RG14 metabolites. Mode of cell death induced by UL4 metabolites on MCF-7 cells was elucidated. Results obtained in trypan blue dye exclusion assay suggested that UL4 metabolites did not cause necrosis. Induction of apoptosis rather than necrosis by UL4 metabolites was evident by the presence of most characteristics of apoptosis such as cell shrinkage, blebbing of cell membrane and fragmentation of DNA and nucleus. Annexin V/PI staining showed that substantial early apoptotic cells were detected in MCF-7 cells treated with UL4 metabolites compared to untreated control group. Cells treated with UL4 metabolites showed growth arrest at G0/G1 cell phase at 24 hours, followed by the increment of cells in sub-G0/G1 in DNA cell cycle analysis. In addition, the TUNEL assay showed that remarkable TUNEL-positive cells were detected in UL4 metabolites-treated MCF-7 cells. The results obtained in this study indicate the potential use of LAB metabolites as a promising antiproliferative and apoptosis induction agent as an alternative in nutraceutical industry and cancer therapy. 2010-12 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/19433/1/FBSB_2010_18_F.pdf Chuah, Li Oon (2010) Cytotoxic and Antiproliferative Properties of Metabolites Produced by Six Strains of Lactobacillus Plantarum on Human Cancer Cells. Masters thesis, Universiti Putra Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Whole cells, cytoplasmic fractions and fermented products of LAB have been tested for anticancer effect. However, limited information is available for the metabolites produced by Lactobacillus plantarum. In this study, the anticancer effect of metabolites produced by six strains of L. plantarum (UL4, TL1, RS5, RG14, RG11 and RI11) isolated from Malaysian fermented foods was evaluated. All metabolites exhibited in vitro cytotoxic effect on the tested cancer cells (breast, colorectal, cervical, liver and leukemia cancer cell lines). An increased cytotoxic effect was observed with increased dose of metabolites used and time of incubation. In particular, metabolites UL4 exerted the most potent cytotoxicity against human breast carcinoma cells MCF-7 in a dose- and time-dependent manner in MTT assay, with inhibition concentration of 50 % growth (IC50) value of 15, 12 and 10% (v/v) for 24, 48 and 72 hours of incubation, respectively. In contrast, no cytotoxicity was detected in primary human peripheral blood mononuclear cells, mouse splenocytes, thymocytes and bone marrow cells for all the six metabolites tested. However, limited cytotoxicity was detected in nonmalignant human glandular epithelium cells MCF-10A when treated with UL4 and RG14 metabolites. Additionally, UL4 metabolites did not cause haemolysis, indicating cytotoxic effect of metabolites of six strains of L. plantarum is selective for malignant cells but spared on normal cells. Antiproliferative effect was focused on MCF-7 and colon cancer cell line (HT-29). In BrdU cell proliferation assay, all tested metabolites inhibited DNA synthesis of MCF-7 and HT-29 cells. An increased antiproliferative effect was observed with increased dose of metabolites used and time of incubation. In particular, UL4 metabolites exhibited 100% proliferation inhibition on MCF-7, whereas RG14 metabolites exhibited 89% proliferation inhibition on HT-29 for 72 hours of incubation. Growth arrest study showed significant cell growth inhibition (P < 0.05) in MCF-7 treated with UL4 metabolites and HT-29 cells treated with RG14 metabolites. Mode of cell death induced by UL4 metabolites on MCF-7 cells was elucidated. Results obtained in trypan blue dye exclusion assay suggested that UL4 metabolites did not cause necrosis. Induction of apoptosis rather than necrosis by UL4 metabolites was evident by the presence of most characteristics of apoptosis such as cell shrinkage, blebbing of cell membrane and fragmentation of DNA and nucleus. Annexin V/PI staining showed that substantial early apoptotic cells were detected in MCF-7 cells treated with UL4 metabolites compared to untreated control group. Cells treated with UL4 metabolites showed growth arrest at G0/G1 cell phase at 24 hours, followed by the increment of cells in sub-G0/G1 in DNA cell cycle analysis. In addition, the TUNEL assay showed that remarkable TUNEL-positive cells were detected in UL4 metabolites-treated MCF-7 cells. The results obtained in this study indicate the potential use of LAB metabolites as a promising antiproliferative and apoptosis induction agent as an alternative in nutraceutical industry and cancer therapy.
format Thesis
author Chuah, Li Oon
spellingShingle Chuah, Li Oon
Cytotoxic and Antiproliferative Properties of Metabolites Produced by Six Strains of Lactobacillus Plantarum on Human Cancer Cells
author_facet Chuah, Li Oon
author_sort Chuah, Li Oon
title Cytotoxic and Antiproliferative Properties of Metabolites Produced by Six Strains of Lactobacillus Plantarum on Human Cancer Cells
title_short Cytotoxic and Antiproliferative Properties of Metabolites Produced by Six Strains of Lactobacillus Plantarum on Human Cancer Cells
title_full Cytotoxic and Antiproliferative Properties of Metabolites Produced by Six Strains of Lactobacillus Plantarum on Human Cancer Cells
title_fullStr Cytotoxic and Antiproliferative Properties of Metabolites Produced by Six Strains of Lactobacillus Plantarum on Human Cancer Cells
title_full_unstemmed Cytotoxic and Antiproliferative Properties of Metabolites Produced by Six Strains of Lactobacillus Plantarum on Human Cancer Cells
title_sort cytotoxic and antiproliferative properties of metabolites produced by six strains of lactobacillus plantarum on human cancer cells
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/19433/1/FBSB_2010_18_F.pdf
http://psasir.upm.edu.my/id/eprint/19433/
_version_ 1643827032298094592
score 13.211869

Similar Items