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Identification of Peptides from a Phage Display Library for Differentiating Newcastle Disease Virus Pathotypes

Newcastle disease virus (NDV) strains can be classified as virulent or avirulent based upon the severity of the disease.Biopanning experiments were performed using a disulfide constrained phage display heptapeptide library against three pathotypes of NDV strains: velogenic (highly virulent), mesoge...

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Main Author: Lee, Tong Chuan
Format: Thesis
Language:English
English
Published: 2006
Online Access:http://psasir.upm.edu.my/id/eprint/194/1/549019_FBSB_2006_12.pdf
http://psasir.upm.edu.my/id/eprint/194/
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spelling my.upm.eprints.1942013-05-27T06:46:25Z http://psasir.upm.edu.my/id/eprint/194/ Identification of Peptides from a Phage Display Library for Differentiating Newcastle Disease Virus Pathotypes Lee, Tong Chuan Newcastle disease virus (NDV) strains can be classified as virulent or avirulent based upon the severity of the disease.Biopanning experiments were performed using a disulfide constrained phage display heptapeptide library against three pathotypes of NDV strains: velogenic (highly virulent), mesogenic (moderately virulent) and lentogenic (avirulent).A phage clone bearing the peptide sequence SWGEYDM was isolated and shown to be able to differentiate virulent from avirulent NDV strains.This phage clone was employed as a capturing reagent in a dot-blot assay to detect virulent NDV strains in allantoic fluid of embryonated chicken eggs. The performance of the dot blot assay was compared with that of mean death time (MDT) in embryonated chicken eggs and the reverse transcription-polymerase chain reaction (RT-PCR) methods.The dot blot was shown to be specific for virulent NDV strains and able to differentiate between the virulent and avirulent NDV strains 2006-03 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/194/1/549019_FBSB_2006_12.pdf Lee, Tong Chuan (2006) Identification of Peptides from a Phage Display Library for Differentiating Newcastle Disease Virus Pathotypes. Masters thesis, Universiti Putra Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Newcastle disease virus (NDV) strains can be classified as virulent or avirulent based upon the severity of the disease.Biopanning experiments were performed using a disulfide constrained phage display heptapeptide library against three pathotypes of NDV strains: velogenic (highly virulent), mesogenic (moderately virulent) and lentogenic (avirulent).A phage clone bearing the peptide sequence SWGEYDM was isolated and shown to be able to differentiate virulent from avirulent NDV strains.This phage clone was employed as a capturing reagent in a dot-blot assay to detect virulent NDV strains in allantoic fluid of embryonated chicken eggs. The performance of the dot blot assay was compared with that of mean death time (MDT) in embryonated chicken eggs and the reverse transcription-polymerase chain reaction (RT-PCR) methods.The dot blot was shown to be specific for virulent NDV strains and able to differentiate between the virulent and avirulent NDV strains
format Thesis
author Lee, Tong Chuan
spellingShingle Lee, Tong Chuan
Identification of Peptides from a Phage Display Library for Differentiating Newcastle Disease Virus Pathotypes
author_facet Lee, Tong Chuan
author_sort Lee, Tong Chuan
title Identification of Peptides from a Phage Display Library for Differentiating Newcastle Disease Virus Pathotypes
title_short Identification of Peptides from a Phage Display Library for Differentiating Newcastle Disease Virus Pathotypes
title_full Identification of Peptides from a Phage Display Library for Differentiating Newcastle Disease Virus Pathotypes
title_fullStr Identification of Peptides from a Phage Display Library for Differentiating Newcastle Disease Virus Pathotypes
title_full_unstemmed Identification of Peptides from a Phage Display Library for Differentiating Newcastle Disease Virus Pathotypes
title_sort identification of peptides from a phage display library for differentiating newcastle disease virus pathotypes
publishDate 2006
url http://psasir.upm.edu.my/id/eprint/194/1/549019_FBSB_2006_12.pdf
http://psasir.upm.edu.my/id/eprint/194/
_version_ 1643821758769266688
score 13.211869

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