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Cytopathology of larval tissues of Mamestra Brassicae L.(Lepidoptera, Noctuidae), infected with a nuclear polyhedrosis virus.

Virus-infected last instar larvae of Mamestra brassicae L. (Lepidoptera, Noctuidae) were collected from a laboratory culture. External symptoms such as white or pink discolouration of the integument, fracture of the cuticle and subsequent release of white body fluids from moribund larvae were indic...

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Main Author: M., Vanhaecke
Format: Conference or Workshop Item
Language:English
English
Published: 1988
Online Access:http://psasir.upm.edu.my/id/eprint/18649/1/ID%2018649.pdf
http://psasir.upm.edu.my/id/eprint/18649/
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spelling my.upm.eprints.186492013-11-04T02:33:36Z http://psasir.upm.edu.my/id/eprint/18649/ Cytopathology of larval tissues of Mamestra Brassicae L.(Lepidoptera, Noctuidae), infected with a nuclear polyhedrosis virus. M., Vanhaecke Virus-infected last instar larvae of Mamestra brassicae L. (Lepidoptera, Noctuidae) were collected from a laboratory culture. External symptoms such as white or pink discolouration of the integument, fracture of the cuticle and subsequent release of white body fluids from moribund larvae were indicative of nuclear polyhedrosis virus (NPV) infection. At the light microscope level occlusion bodies of the Mamestra brassicaeNPV were observed in the nuclei of the visceral fat body, the matrik cells of the tracheae and the epidermal cells. In the early stages of infection and virus replication, nuclei are enlarged and filled with virogenic stroma. Fat body cells lack the osmophilic fat droplets in their cytoplasm at this stage. Later occlusion bodies appear in the nucleoplasma and finally greatly enlarged nuclei, filled with polyhedra, occupy almost the entire cell volume. Nuclear and cell membranes burst and their contents together with the viral polyhedra mix with the haemolymph. At the electron microscope level, other pathological changes inside the nucleus are the infolding and concurrent reduplication of the inner nuclear membrane. In the cytoplasm of infected cells mitochondria are swollen and eventually are subjected to cristolysis. Tubuloreticular membrane structures are associated with them. 1988 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18649/1/ID%2018649.pdf M., Vanhaecke (1988) Cytopathology of larval tissues of Mamestra Brassicae L.(Lepidoptera, Noctuidae), infected with a nuclear polyhedrosis virus. In: 11th Malaysian Microbiology Symposium, 22-23 Aug 1988, Kuala Lumpur. (pp. 1-11). (Unpublished) English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Virus-infected last instar larvae of Mamestra brassicae L. (Lepidoptera, Noctuidae) were collected from a laboratory culture. External symptoms such as white or pink discolouration of the integument, fracture of the cuticle and subsequent release of white body fluids from moribund larvae were indicative of nuclear polyhedrosis virus (NPV) infection. At the light microscope level occlusion bodies of the Mamestra brassicaeNPV were observed in the nuclei of the visceral fat body, the matrik cells of the tracheae and the epidermal cells. In the early stages of infection and virus replication, nuclei are enlarged and filled with virogenic stroma. Fat body cells lack the osmophilic fat droplets in their cytoplasm at this stage. Later occlusion bodies appear in the nucleoplasma and finally greatly enlarged nuclei, filled with polyhedra, occupy almost the entire cell volume. Nuclear and cell membranes burst and their contents together with the viral polyhedra mix with the haemolymph. At the electron microscope level, other pathological changes inside the nucleus are the infolding and concurrent reduplication of the inner nuclear membrane. In the cytoplasm of infected cells mitochondria are swollen and eventually are subjected to cristolysis. Tubuloreticular membrane structures are associated with them.
format Conference or Workshop Item
author M., Vanhaecke
spellingShingle M., Vanhaecke
Cytopathology of larval tissues of Mamestra Brassicae L.(Lepidoptera, Noctuidae), infected with a nuclear polyhedrosis virus.
author_facet M., Vanhaecke
author_sort M., Vanhaecke
title Cytopathology of larval tissues of Mamestra Brassicae L.(Lepidoptera, Noctuidae), infected with a nuclear polyhedrosis virus.
title_short Cytopathology of larval tissues of Mamestra Brassicae L.(Lepidoptera, Noctuidae), infected with a nuclear polyhedrosis virus.
title_full Cytopathology of larval tissues of Mamestra Brassicae L.(Lepidoptera, Noctuidae), infected with a nuclear polyhedrosis virus.
title_fullStr Cytopathology of larval tissues of Mamestra Brassicae L.(Lepidoptera, Noctuidae), infected with a nuclear polyhedrosis virus.
title_full_unstemmed Cytopathology of larval tissues of Mamestra Brassicae L.(Lepidoptera, Noctuidae), infected with a nuclear polyhedrosis virus.
title_sort cytopathology of larval tissues of mamestra brassicae l.(lepidoptera, noctuidae), infected with a nuclear polyhedrosis virus.
publishDate 1988
url http://psasir.upm.edu.my/id/eprint/18649/1/ID%2018649.pdf
http://psasir.upm.edu.my/id/eprint/18649/
_version_ 1643826857890545664
score 13.211869

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