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Immunomodulatory properties of bacteriocin UL4 from lactobacillus plantarum contribute to reduced incidence of colon cancer in rats

Probiotics are known to modulate immunity in animals and human subjects, and to inhibit colon carcinogenesis in experimental models. Nevertheless, the effects of metabolites from the probiotics are not well understood. In this study, the effects of bacteriocin UL4 (UL4), a metabolite from probiotic...

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Main Authors: Saiful Yazan, Latifah, M. Z., Noraina, Foo, H. L., Mohammed Alitheen, Noorjahan Banu, M., Hair-Bejo, M., Saidi
Format: Conference or Workshop Item
Published: 2009
Online Access:http://psasir.upm.edu.my/id/eprint/18483/
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spelling my.upm.eprints.184832016-03-01T08:59:06Z http://psasir.upm.edu.my/id/eprint/18483/ Immunomodulatory properties of bacteriocin UL4 from lactobacillus plantarum contribute to reduced incidence of colon cancer in rats Saiful Yazan, Latifah M. Z., Noraina Foo, H. L. Mohammed Alitheen, Noorjahan Banu M., Hair-Bejo M., Saidi Probiotics are known to modulate immunity in animals and human subjects, and to inhibit colon carcinogenesis in experimental models. Nevertheless, the effects of metabolites from the probiotics are not well understood. In this study, the effects of bacteriocin UL4 (UL4), a metabolite from probiotic Lactobacillus plantarum on the immune system were investigated in the azoxymethane (AOM)-induced colon cancer rats. Briefly, 60 male Sprague Dawley rats 6 weeks of age with average body weight of 90-100 g were induced with azoxymethane (AOM) (15 mg/kg body) subcutaneously, once a week for two weeks. Following that, the rats were randomly divided into 5 groups (15 rats per group) ,which were (1) positive control (induced with colon cancer, unfed with UL4); (2) fed with 0.25% of UL4; (3) fed with 0.5% of UL4; (4) fed with 0.75% of UL4 and (5) negative control (not induced with colon cancer, unfed with UL4). After 6 months induction of colon cancer, rats were treated with different percentages of UL4 for 12 weeks. After 36 weeks, all rats were killed and blood was withdrawn. The immune cell suspensions were prepared from spleen and thymus of the rats. All groups treated with UL4 showed significant increased level of tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ) in the serum, spleen and thymus cell suspensions for 24, 48 and 72 hours of incubation time compared to the positive control group (p<0.05). In conclusion, UL4 stimulates the secretion of TNFα and IFNγ, leading to the activation of specific immune responses contributing to its role in inhibition of tumorigenesis (reduced crypt multiplicity and number of ACF in the rats induced with colon cancer). 2009-12 Conference or Workshop Item NonPeerReviewed Saiful Yazan, Latifah and M. Z., Noraina and Foo, H. L. and Mohammed Alitheen, Noorjahan Banu and M., Hair-Bejo and M., Saidi (2009) Immunomodulatory properties of bacteriocin UL4 from lactobacillus plantarum contribute to reduced incidence of colon cancer in rats. In: BIT's 3rd World Congress of Gene 2009, 1-7 Dis 2009, Foshan, China. .
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Probiotics are known to modulate immunity in animals and human subjects, and to inhibit colon carcinogenesis in experimental models. Nevertheless, the effects of metabolites from the probiotics are not well understood. In this study, the effects of bacteriocin UL4 (UL4), a metabolite from probiotic Lactobacillus plantarum on the immune system were investigated in the azoxymethane (AOM)-induced colon cancer rats. Briefly, 60 male Sprague Dawley rats 6 weeks of age with average body weight of 90-100 g were induced with azoxymethane (AOM) (15 mg/kg body) subcutaneously, once a week for two weeks. Following that, the rats were randomly divided into 5 groups (15 rats per group) ,which were (1) positive control (induced with colon cancer, unfed with UL4); (2) fed with 0.25% of UL4; (3) fed with 0.5% of UL4; (4) fed with 0.75% of UL4 and (5) negative control (not induced with colon cancer, unfed with UL4). After 6 months induction of colon cancer, rats were treated with different percentages of UL4 for 12 weeks. After 36 weeks, all rats were killed and blood was withdrawn. The immune cell suspensions were prepared from spleen and thymus of the rats. All groups treated with UL4 showed significant increased level of tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ) in the serum, spleen and thymus cell suspensions for 24, 48 and 72 hours of incubation time compared to the positive control group (p<0.05). In conclusion, UL4 stimulates the secretion of TNFα and IFNγ, leading to the activation of specific immune responses contributing to its role in inhibition of tumorigenesis (reduced crypt multiplicity and number of ACF in the rats induced with colon cancer).
format Conference or Workshop Item
author Saiful Yazan, Latifah
M. Z., Noraina
Foo, H. L.
Mohammed Alitheen, Noorjahan Banu
M., Hair-Bejo
M., Saidi
spellingShingle Saiful Yazan, Latifah
M. Z., Noraina
Foo, H. L.
Mohammed Alitheen, Noorjahan Banu
M., Hair-Bejo
M., Saidi
Immunomodulatory properties of bacteriocin UL4 from lactobacillus plantarum contribute to reduced incidence of colon cancer in rats
author_facet Saiful Yazan, Latifah
M. Z., Noraina
Foo, H. L.
Mohammed Alitheen, Noorjahan Banu
M., Hair-Bejo
M., Saidi
author_sort Saiful Yazan, Latifah
title Immunomodulatory properties of bacteriocin UL4 from lactobacillus plantarum contribute to reduced incidence of colon cancer in rats
title_short Immunomodulatory properties of bacteriocin UL4 from lactobacillus plantarum contribute to reduced incidence of colon cancer in rats
title_full Immunomodulatory properties of bacteriocin UL4 from lactobacillus plantarum contribute to reduced incidence of colon cancer in rats
title_fullStr Immunomodulatory properties of bacteriocin UL4 from lactobacillus plantarum contribute to reduced incidence of colon cancer in rats
title_full_unstemmed Immunomodulatory properties of bacteriocin UL4 from lactobacillus plantarum contribute to reduced incidence of colon cancer in rats
title_sort immunomodulatory properties of bacteriocin ul4 from lactobacillus plantarum contribute to reduced incidence of colon cancer in rats
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/18483/
_version_ 1643826816555679744
score 13.211869

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