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The identification and characterization of a novel transcription factor LeCBP, a potential regulator of the tomato stylar Chi2;1 promoter

A gene, LeCBP encoding a protein containing a PHD zinc finger motif and a bipartite nuclear localization signal, was identified from tomato pistils by its binding ability to a specific DNA motif using a yeast one-hybrid system. The LeCBP has the ability to bind specifically to a 20 bp region of the...

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Main Authors: Chan, Pick Kuen, Mohd Yusuf, Hirzun, Wong, Clemente Michael Vui Ling, Kulaveerasingam, Harikrishna
Format: Article
Language:English
English
Published: Elsevier 2004
Online Access:http://psasir.upm.edu.my/id/eprint/18404/1/The%20identification%20and%20characterization%20of%20a%20novel%20transcription%20factor%20LeCBP.pdf
http://psasir.upm.edu.my/id/eprint/18404/
http://dx.doi.org/10.1016/j.plantsci.2004.06.032
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spelling my.upm.eprints.184042015-11-27T02:01:36Z http://psasir.upm.edu.my/id/eprint/18404/ The identification and characterization of a novel transcription factor LeCBP, a potential regulator of the tomato stylar Chi2;1 promoter Chan, Pick Kuen Mohd Yusuf, Hirzun Wong, Clemente Michael Vui Ling Kulaveerasingam, Harikrishna A gene, LeCBP encoding a protein containing a PHD zinc finger motif and a bipartite nuclear localization signal, was identified from tomato pistils by its binding ability to a specific DNA motif using a yeast one-hybrid system. The LeCBP has the ability to bind specifically to a 20 bp region of the promoter of Chi2;1, encoding a pistil chitinase. LeCBP also has the ability to activate the yeast URA3 reporter gene in yeast. An analysis of the temporal and spatial patterns of gene expression through northern blotting and in situ hybridization has demonstrated the highest levels of expression in mature tomato pistils where the mRNA was localized along the transmitting tracks of the style as well as on the ovules and ovary walls. The specific binding property of LeCBP with Chi2;1 promoter may be used to engineer high levels of expression in pistils of transgenic plants. © 2004 Elsevier Ireland Ltd. All rights reserved. Elsevier 2004-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18404/1/The%20identification%20and%20characterization%20of%20a%20novel%20transcription%20factor%20LeCBP.pdf Chan, Pick Kuen and Mohd Yusuf, Hirzun and Wong, Clemente Michael Vui Ling and Kulaveerasingam, Harikrishna (2004) The identification and characterization of a novel transcription factor LeCBP, a potential regulator of the tomato stylar Chi2;1 promoter. Plant Science, 167 (6). pp. 1291-1300. ISSN 0168-9452 http://dx.doi.org/10.1016/j.plantsci.2004.06.032 doi:10.1016/j.plantsci.2004.06.032 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description A gene, LeCBP encoding a protein containing a PHD zinc finger motif and a bipartite nuclear localization signal, was identified from tomato pistils by its binding ability to a specific DNA motif using a yeast one-hybrid system. The LeCBP has the ability to bind specifically to a 20 bp region of the promoter of Chi2;1, encoding a pistil chitinase. LeCBP also has the ability to activate the yeast URA3 reporter gene in yeast. An analysis of the temporal and spatial patterns of gene expression through northern blotting and in situ hybridization has demonstrated the highest levels of expression in mature tomato pistils where the mRNA was localized along the transmitting tracks of the style as well as on the ovules and ovary walls. The specific binding property of LeCBP with Chi2;1 promoter may be used to engineer high levels of expression in pistils of transgenic plants. © 2004 Elsevier Ireland Ltd. All rights reserved.
format Article
author Chan, Pick Kuen
Mohd Yusuf, Hirzun
Wong, Clemente Michael Vui Ling
Kulaveerasingam, Harikrishna
spellingShingle Chan, Pick Kuen
Mohd Yusuf, Hirzun
Wong, Clemente Michael Vui Ling
Kulaveerasingam, Harikrishna
The identification and characterization of a novel transcription factor LeCBP, a potential regulator of the tomato stylar Chi2;1 promoter
author_facet Chan, Pick Kuen
Mohd Yusuf, Hirzun
Wong, Clemente Michael Vui Ling
Kulaveerasingam, Harikrishna
author_sort Chan, Pick Kuen
title The identification and characterization of a novel transcription factor LeCBP, a potential regulator of the tomato stylar Chi2;1 promoter
title_short The identification and characterization of a novel transcription factor LeCBP, a potential regulator of the tomato stylar Chi2;1 promoter
title_full The identification and characterization of a novel transcription factor LeCBP, a potential regulator of the tomato stylar Chi2;1 promoter
title_fullStr The identification and characterization of a novel transcription factor LeCBP, a potential regulator of the tomato stylar Chi2;1 promoter
title_full_unstemmed The identification and characterization of a novel transcription factor LeCBP, a potential regulator of the tomato stylar Chi2;1 promoter
title_sort identification and characterization of a novel transcription factor lecbp, a potential regulator of the tomato stylar chi2;1 promoter
publisher Elsevier
publishDate 2004
url http://psasir.upm.edu.my/id/eprint/18404/1/The%20identification%20and%20characterization%20of%20a%20novel%20transcription%20factor%20LeCBP.pdf
http://psasir.upm.edu.my/id/eprint/18404/
http://dx.doi.org/10.1016/j.plantsci.2004.06.032
_version_ 1643826793321332736
score 13.211869

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