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Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch

Enhancement of pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium formulation was investigated in batch fermentation using 500-mL shake flask. The fermentations were carried out, firstly, to search for a suitable cultivation medium for enzyme production and followed b...

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Main Authors: Siew, Ling Hii, Tau, Chuan Ling, Mohamad, Rosfarizan, Ariff, Arbakariya
Format: Article
Language:English
Published: Bentham Science Publishers 2008
Online Access:http://psasir.upm.edu.my/id/eprint/17701/
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spelling my.upm.eprints.177012011-12-01T05:21:41Z http://psasir.upm.edu.my/id/eprint/17701/ Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch Siew, Ling Hii Tau, Chuan Ling Mohamad, Rosfarizan Ariff, Arbakariya Enhancement of pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium formulation was investigated in batch fermentation using 500-mL shake flask. The fermentations were carried out, firstly, to search for a suitable cultivation medium for enzyme production and followed by the evaluations on the influence of carbon and nitrogen sources and also initial culture pHs on the secretion of pullulanase by this bacterium. The modified mineral Czapek medium was found suitable to produce substantially high activity of pullulanase (320 times higher) as compared to ‘Ohba-Ueda’ medium. This bacterium was found superior in pullulanase production using sago starch and peptone as carbon and nitrogen sources, respectively. Using the optimized medium, the bacterium produced 0.95 U/mL of pullulanase at initial culture pH of 7 and incubation temperature of 30oC. Bentham Science Publishers 2008 Article PeerReviewed Siew, Ling Hii and Tau, Chuan Ling and Mohamad, Rosfarizan and Ariff, Arbakariya (2008) Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch. The Open Biotechnology Journal, 3. pp. 1-8. ISSN 1874-0707 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Enhancement of pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium formulation was investigated in batch fermentation using 500-mL shake flask. The fermentations were carried out, firstly, to search for a suitable cultivation medium for enzyme production and followed by the evaluations on the influence of carbon and nitrogen sources and also initial culture pHs on the secretion of pullulanase by this bacterium. The modified mineral Czapek medium was found suitable to produce substantially high activity of pullulanase (320 times higher) as compared to ‘Ohba-Ueda’ medium. This bacterium was found superior in pullulanase production using sago starch and peptone as carbon and nitrogen sources, respectively. Using the optimized medium, the bacterium produced 0.95 U/mL of pullulanase at initial culture pH of 7 and incubation temperature of 30oC.
format Article
author Siew, Ling Hii
Tau, Chuan Ling
Mohamad, Rosfarizan
Ariff, Arbakariya
spellingShingle Siew, Ling Hii
Tau, Chuan Ling
Mohamad, Rosfarizan
Ariff, Arbakariya
Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch
author_facet Siew, Ling Hii
Tau, Chuan Ling
Mohamad, Rosfarizan
Ariff, Arbakariya
author_sort Siew, Ling Hii
title Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch
title_short Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch
title_full Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch
title_fullStr Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch
title_full_unstemmed Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch
title_sort enhancement of extracellular pullulanase production by raoultella planticola dsmz 4617 using optimized medium based on sago starch
publisher Bentham Science Publishers
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/17701/
_version_ 1643826599158611968
score 13.211869

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