Cover Image

Toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro.

Cationic polymers are a leading class of nonviral self-assembled nucleic acid delivery systems. Cationic polymers have been shown to condence the DNA so that the entrapped DNA is protected from contact with DNase. The objective of the present study is to evaluate the effect of cationic dextran on th...

Full description

Saved in:
Bibliographic Details
Main Authors: Abedini, Fatemah, Ismail, Maznah, Hosseinkhani, Hossein, Tengku Ibrahim, Tengku Azmi, Omar, Abdolrahman, Chong, Pei Pei, Ismail, Norsharina, Farber, Ira-Yudovin, Domb, Abraham J.
Format: Article
Language:English
English
Published: Academic Journals 2010
Online Access:http://psasir.upm.edu.my/id/eprint/17451/1/Toxicity%20evaluation%20of%20dextran.pdf
http://psasir.upm.edu.my/id/eprint/17451/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.17451
record_format eprints
spelling my.upm.eprints.174512015-11-05T08:07:27Z http://psasir.upm.edu.my/id/eprint/17451/ Toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro. Abedini, Fatemah Ismail, Maznah Hosseinkhani, Hossein Tengku Ibrahim, Tengku Azmi Omar, Abdolrahman Chong, Pei Pei Ismail, Norsharina Farber, Ira-Yudovin Domb, Abraham J. Cationic polymers are a leading class of nonviral self-assembled nucleic acid delivery systems. Cationic polymers have been shown to condence the DNA so that the entrapped DNA is protected from contact with DNase. The objective of the present study is to evaluate the effect of cationic dextran on the proliferation rate, morphological changes and biosynthetic activities in vitro. Cationic dextran was prepared by means of reductive-amination between oxidized dextran and the natural oligoamine, spermine. Four kinds of biological evaluations including cell proliferation assay, ultrastructural changes of cells using transmission electron microscopy (TEM), acridine orange/Propidium Iodide and cell cycle were studied. Our results clearly indicated that the toxicity of cationic dextran is dose depended and it is not toxic at low concentration and tolerable by the cells, and it can be used as a tool for gene delivery. Academic Journals 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17451/1/Toxicity%20evaluation%20of%20dextran.pdf Abedini, Fatemah and Ismail, Maznah and Hosseinkhani, Hossein and Tengku Ibrahim, Tengku Azmi and Omar, Abdolrahman and Chong, Pei Pei and Ismail, Norsharina and Farber, Ira-Yudovin and Domb, Abraham J. (2010) Toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro. Journal of Cell and Animal Biology, 4 (12). pp. 170-176. ISSN 1996-0867 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Cationic polymers are a leading class of nonviral self-assembled nucleic acid delivery systems. Cationic polymers have been shown to condence the DNA so that the entrapped DNA is protected from contact with DNase. The objective of the present study is to evaluate the effect of cationic dextran on the proliferation rate, morphological changes and biosynthetic activities in vitro. Cationic dextran was prepared by means of reductive-amination between oxidized dextran and the natural oligoamine, spermine. Four kinds of biological evaluations including cell proliferation assay, ultrastructural changes of cells using transmission electron microscopy (TEM), acridine orange/Propidium Iodide and cell cycle were studied. Our results clearly indicated that the toxicity of cationic dextran is dose depended and it is not toxic at low concentration and tolerable by the cells, and it can be used as a tool for gene delivery.
format Article
author Abedini, Fatemah
Ismail, Maznah
Hosseinkhani, Hossein
Tengku Ibrahim, Tengku Azmi
Omar, Abdolrahman
Chong, Pei Pei
Ismail, Norsharina
Farber, Ira-Yudovin
Domb, Abraham J.
spellingShingle Abedini, Fatemah
Ismail, Maznah
Hosseinkhani, Hossein
Tengku Ibrahim, Tengku Azmi
Omar, Abdolrahman
Chong, Pei Pei
Ismail, Norsharina
Farber, Ira-Yudovin
Domb, Abraham J.
Toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro.
author_facet Abedini, Fatemah
Ismail, Maznah
Hosseinkhani, Hossein
Tengku Ibrahim, Tengku Azmi
Omar, Abdolrahman
Chong, Pei Pei
Ismail, Norsharina
Farber, Ira-Yudovin
Domb, Abraham J.
author_sort Abedini, Fatemah
title Toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro.
title_short Toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro.
title_full Toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro.
title_fullStr Toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro.
title_full_unstemmed Toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro.
title_sort toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro.
publisher Academic Journals
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/17451/1/Toxicity%20evaluation%20of%20dextran.pdf
http://psasir.upm.edu.my/id/eprint/17451/
_version_ 1643826520913870848
score 13.211869

Similar Items