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Analysis of peripheral blood of ovarian cancer patients indicates higher sub-populations of natural killer and B cells compared to healthy volunteers

Ovarian cancer is a challenging disease to treat, and one of the potential treatments is by immunotherapy. NK cells have been shown to play a role in slowing tumour progression and cancer development. This study aims to investigate the numbers of NK cells and other lymphocyte sub-populations in ovar...

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Main Authors: Tan, Jun Hao, Visvalingam, Vicknesh, Mat Adenan, Noor Azmi, Mohtaruddin, Norhafizah, Abdullah, Maha, Andi Asri, Andi Anggeriana
Format: Conference or Workshop Item
Language:English
Published: UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience 2014
Online Access:http://psasir.upm.edu.my/id/eprint/17225/1/ABSTRACT%20CAC%202014_2%20medic%2013.pdf
http://psasir.upm.edu.my/id/eprint/17225/
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spelling my.upm.eprints.172252016-04-26T03:54:03Z http://psasir.upm.edu.my/id/eprint/17225/ Analysis of peripheral blood of ovarian cancer patients indicates higher sub-populations of natural killer and B cells compared to healthy volunteers Tan, Jun Hao Visvalingam, Vicknesh Mat Adenan, Noor Azmi Mohtaruddin, Norhafizah Abdullah, Maha Andi Asri, Andi Anggeriana Ovarian cancer is a challenging disease to treat, and one of the potential treatments is by immunotherapy. NK cells have been shown to play a role in slowing tumour progression and cancer development. This study aims to investigate the numbers of NK cells and other lymphocyte sub-populations in ovarian cancer and their impact on ovarian cancer clinical outcome. This project aims to study the significance of different lymphocyte populations, particularly NK cells, involved in the peripheral blood of ovarian cancer patients. Venal blood was drawn from ovarian cancer patients before chemotherapy. PBMCs were isolated from 13 ovarian cancer patients and 11 age-matched healthy volunteers. Immunophenotyping was performed using a commercial kit to quantify the lymphocyte populations and RNA isolation performed to examine the expression of KIR genes using reverse transcription polymerase chain reaction. Immunophenotyping of PBMC was successfully performed on 13 ovarian cancer patients and 11 healthy controls. Significant increases in the mean of peripheral NK cells and B cells were found in ovarian cancer patients as compared to the healthy controls (P=0.0559). No other significant results were obtained for C D4 and CD8 lymphocytes. There was significant increase in numbers of NK cells and B cells in ovarian cancer patients as compared to the healthy volunteers. These results should be pursued with a larger sample size with the hopes of finding a significant difference between the two groups and to provide a keener insight into are promising preliminary results the immune defence against ovarian cancer. UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience 2014 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17225/1/ABSTRACT%20CAC%202014_2%20medic%2013.pdf Tan, Jun Hao and Visvalingam, Vicknesh and Mat Adenan, Noor Azmi and Mohtaruddin, Norhafizah and Abdullah, Maha and Andi Asri, Andi Anggeriana (2014) Analysis of peripheral blood of ovarian cancer patients indicates higher sub-populations of natural killer and B cells compared to healthy volunteers. In: Scientific Cancer Research Poster Competition in conjunction with Cancer Awareness Carnival 2014, 10 May 2014, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia. .
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Ovarian cancer is a challenging disease to treat, and one of the potential treatments is by immunotherapy. NK cells have been shown to play a role in slowing tumour progression and cancer development. This study aims to investigate the numbers of NK cells and other lymphocyte sub-populations in ovarian cancer and their impact on ovarian cancer clinical outcome. This project aims to study the significance of different lymphocyte populations, particularly NK cells, involved in the peripheral blood of ovarian cancer patients. Venal blood was drawn from ovarian cancer patients before chemotherapy. PBMCs were isolated from 13 ovarian cancer patients and 11 age-matched healthy volunteers. Immunophenotyping was performed using a commercial kit to quantify the lymphocyte populations and RNA isolation performed to examine the expression of KIR genes using reverse transcription polymerase chain reaction. Immunophenotyping of PBMC was successfully performed on 13 ovarian cancer patients and 11 healthy controls. Significant increases in the mean of peripheral NK cells and B cells were found in ovarian cancer patients as compared to the healthy controls (P=0.0559). No other significant results were obtained for C D4 and CD8 lymphocytes. There was significant increase in numbers of NK cells and B cells in ovarian cancer patients as compared to the healthy volunteers. These results should be pursued with a larger sample size with the hopes of finding a significant difference between the two groups and to provide a keener insight into are promising preliminary results the immune defence against ovarian cancer.
format Conference or Workshop Item
author Tan, Jun Hao
Visvalingam, Vicknesh
Mat Adenan, Noor Azmi
Mohtaruddin, Norhafizah
Abdullah, Maha
Andi Asri, Andi Anggeriana
spellingShingle Tan, Jun Hao
Visvalingam, Vicknesh
Mat Adenan, Noor Azmi
Mohtaruddin, Norhafizah
Abdullah, Maha
Andi Asri, Andi Anggeriana
Analysis of peripheral blood of ovarian cancer patients indicates higher sub-populations of natural killer and B cells compared to healthy volunteers
author_facet Tan, Jun Hao
Visvalingam, Vicknesh
Mat Adenan, Noor Azmi
Mohtaruddin, Norhafizah
Abdullah, Maha
Andi Asri, Andi Anggeriana
author_sort Tan, Jun Hao
title Analysis of peripheral blood of ovarian cancer patients indicates higher sub-populations of natural killer and B cells compared to healthy volunteers
title_short Analysis of peripheral blood of ovarian cancer patients indicates higher sub-populations of natural killer and B cells compared to healthy volunteers
title_full Analysis of peripheral blood of ovarian cancer patients indicates higher sub-populations of natural killer and B cells compared to healthy volunteers
title_fullStr Analysis of peripheral blood of ovarian cancer patients indicates higher sub-populations of natural killer and B cells compared to healthy volunteers
title_full_unstemmed Analysis of peripheral blood of ovarian cancer patients indicates higher sub-populations of natural killer and B cells compared to healthy volunteers
title_sort analysis of peripheral blood of ovarian cancer patients indicates higher sub-populations of natural killer and b cells compared to healthy volunteers
publisher UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/17225/1/ABSTRACT%20CAC%202014_2%20medic%2013.pdf
http://psasir.upm.edu.my/id/eprint/17225/
_version_ 1643826451614531584
score 13.211869

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