Statistical optimization of the induction of the induction of phytase production by Arabinose in a recombinant E. coli using response surface methodology
The production of phytase in a recombinant E.coli using the pBAD expression system was optimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentration in the cultivation media and the incubation temperatu...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
ThaiScience
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16840/1/Statistical%20optimization%20of%20the%20induction%20of%20the%20induction%20of%20phytase%20production%20by%20Arabinose%20in%20a%20recombinant%20E.pdf http://psasir.upm.edu.my/id/eprint/16840/ http://www.thaiscience.info/journals/view3.asp?sCode=ASTD&sType=JOURNAL#Year 2009, Volume 26 |
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| Summary: | The production of phytase in a recombinant E.coli using the pBAD expression
system was optimized using response surface methodology with full-factorial faced
centered central composite design. The ampicilin and arabinose concentration in the
cultivation media and the incubation temperature were optimized in order to maximize
phytase production using 23
central composite experimental design. With this design the
number of actual experiment performed could be reduced while allowing eludidation of
possible interactions among these factors. The most significant parameter was shown to
be the linear and quadratic effect of the incubation temperature. Optimal conditions for
phytase production were determined to be 100 µg/ml ampicilin, 0.2 % arabinose and an
incubation temperature of 37ºC. The production of phytase in the recombinant E. coli was
scaled up to 100 ml and 1000 ml. |
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